Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions
Exosomes represent an important mode of intercellular communication and play key roles in many physiological and pathological processes. Exosomes have hitherto exhibited their capacity to modulate biological activities through their carrying of functional molecules such as proteins, lipids, and gen...
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| Format: | Article |
| Language: | English |
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Vietnam Ministry of Science and Technology
2022-03-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
| Subjects: | |
| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/169 |
| _version_ | 1828047874280128512 |
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| author | Hoang Huong Diem Bui Thi Van Khanh Hoang Thi My Nhung Nguyen Thanh Liem Than Thi Trang Uyen |
| author_facet | Hoang Huong Diem Bui Thi Van Khanh Hoang Thi My Nhung Nguyen Thanh Liem Than Thi Trang Uyen |
| author_sort | Hoang Huong Diem |
| collection | DOAJ |
| description |
Exosomes represent an important mode of intercellular communication and play key roles in many physiological and pathological processes. Exosomes have hitherto exhibited their capacity to modulate biological activities through their carrying of functional molecules such as proteins, lipids, and genetic materials. In the current study, we investigated exosomes released by mature dendritic cells (mDCs) and umbilical cord-derived mesenchymal stem cells (UCMSCs) under Good Manufacturing Practice (GMP) conditions. Ultracentrifugation was used to isolate and purify the exosomes. Additionally, a transmission electron microscope (TEM) and immunoblotting were used to characterise exosomal morphology and markers. The preliminary results showed that both mDCs and UCMSCs secreted exosomes into GMP culture media. Exosomes exhibited a cup-shaped morphology and showed positive for CD63. Additionally, no difference was observed between mDC-derived exosomes and UCMSC-derived exosomes regarding marker expression or morphology. This data indicates the potential for further development of GMP exosomes for clinical application.
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| first_indexed | 2024-04-10T18:50:00Z |
| format | Article |
| id | doaj.art-10330f05ab604f5bae0d75c0b0512444 |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-10T18:50:00Z |
| publishDate | 2022-03-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-10330f05ab604f5bae0d75c0b05124442023-02-01T08:13:02ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372022-03-0161310.31276/VJSTE.61(3).45-51Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditionsHoang Huong Diem0Bui Thi Van Khanh1Hoang Thi My Nhung2Nguyen Thanh Liem3Than Thi Trang Uyen4University of Science, Vietnam National University, Ha Noi; Vinmec Research Institute of Stem Cell and Gene TechnologyUniversity of Science, Vietnam National University, Ha NoiUniversity of Science, Vietnam National University, Ha Noi; Vinmec Research Institute of Stem Cell and Gene TechnologyVinmec Research Institute of Stem Cell and Gene TechnologyVinmec Research Institute of Stem Cell and Gene Technology Exosomes represent an important mode of intercellular communication and play key roles in many physiological and pathological processes. Exosomes have hitherto exhibited their capacity to modulate biological activities through their carrying of functional molecules such as proteins, lipids, and genetic materials. In the current study, we investigated exosomes released by mature dendritic cells (mDCs) and umbilical cord-derived mesenchymal stem cells (UCMSCs) under Good Manufacturing Practice (GMP) conditions. Ultracentrifugation was used to isolate and purify the exosomes. Additionally, a transmission electron microscope (TEM) and immunoblotting were used to characterise exosomal morphology and markers. The preliminary results showed that both mDCs and UCMSCs secreted exosomes into GMP culture media. Exosomes exhibited a cup-shaped morphology and showed positive for CD63. Additionally, no difference was observed between mDC-derived exosomes and UCMSC-derived exosomes regarding marker expression or morphology. This data indicates the potential for further development of GMP exosomes for clinical application. https://vietnamscience.vjst.vn/index.php/vjste/article/view/169dendritic cellsexosomesmesenchymal stem cells |
| spellingShingle | Hoang Huong Diem Bui Thi Van Khanh Hoang Thi My Nhung Nguyen Thanh Liem Than Thi Trang Uyen Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions Vietnam Journal of Science, Technology and Engineering dendritic cells exosomes mesenchymal stem cells |
| title | Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions |
| title_full | Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions |
| title_fullStr | Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions |
| title_full_unstemmed | Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions |
| title_short | Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions |
| title_sort | initial characterisation of exosomes released by umbilical cord derived mesenchymal stem cells and mature dendritic cells under good manufacturing practice conditions |
| topic | dendritic cells exosomes mesenchymal stem cells |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/169 |
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