Lentil Landrace Seed Origin and Genotype Affects Rhizosphere Microbiome
Lentil (<i>Lens culinaris</i> Medik.) is an essential legume crop providing healthy and nutritious food for people in low- to middle-income countries, worldwide. Lentil roots support symbiotic interactions with soil rhizobia species fostering nitrogen fixation; however, assemblage and di...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/12/2910 |
| _version_ | 1797382353589370880 |
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| author | Anthoula Gleridou Georgios Giannopoulos Alexios N. Polidoros Photini V. Mylona |
| author_facet | Anthoula Gleridou Georgios Giannopoulos Alexios N. Polidoros Photini V. Mylona |
| author_sort | Anthoula Gleridou |
| collection | DOAJ |
| description | Lentil (<i>Lens culinaris</i> Medik.) is an essential legume crop providing healthy and nutritious food for people in low- to middle-income countries, worldwide. Lentil roots support symbiotic interactions with soil rhizobia species fostering nitrogen fixation; however, assemblage and diversity of the complete microbial rhizosphere community and the effect of seed genotype and origin remain largely unexplored. In this study we examined, via metagenomic analysis, the effects of seed origin on the rhizosphere’s communities in samples of the famous Greek lentil landrace, Eglouvis, derived from different local farmers and farming systems (including a Gene Bank sample), in comparison to a commercial variety. The landrace exhibited higher rhizosphere microbiome diversity compared to the commercial variety for all indexes. A core microbiome comprised of 158 taxa was present in all samples, while a greater number of unique bacterial taxa was recorded in the landrace samples compared to the commercial cultivar. Notably, landrace samples originated from organic farming had more than double the number of unique taxa compared to conventional counterparts. The study revealed a higher diversity of N<sub>2</sub> fixers and archaea, <i>Crenarchaeota</i> and <i>Thaumarchaeota</i>, in landrace samples and particularly in those derived from organic farming, underpinning the distinct recruiting efficiency of beneficial soil microbes by the landrace. |
| first_indexed | 2024-03-08T21:04:10Z |
| format | Article |
| id | doaj.art-103365b52b4d43bc932c6354710f1431 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-08T21:04:10Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-103365b52b4d43bc932c6354710f14312023-12-22T13:46:18ZengMDPI AGAgronomy2073-43952023-11-011312291010.3390/agronomy13122910Lentil Landrace Seed Origin and Genotype Affects Rhizosphere MicrobiomeAnthoula Gleridou0Georgios Giannopoulos1Alexios N. Polidoros2Photini V. Mylona3Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Genetics and Plant Breeding, Faculty of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Genetics and Plant Breeding, Faculty of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceInstitute of Plant Breeding & Genetic Resources, Hellenic Agricultural Organization-DEMETER, 57001 Thermi, GreeceLentil (<i>Lens culinaris</i> Medik.) is an essential legume crop providing healthy and nutritious food for people in low- to middle-income countries, worldwide. Lentil roots support symbiotic interactions with soil rhizobia species fostering nitrogen fixation; however, assemblage and diversity of the complete microbial rhizosphere community and the effect of seed genotype and origin remain largely unexplored. In this study we examined, via metagenomic analysis, the effects of seed origin on the rhizosphere’s communities in samples of the famous Greek lentil landrace, Eglouvis, derived from different local farmers and farming systems (including a Gene Bank sample), in comparison to a commercial variety. The landrace exhibited higher rhizosphere microbiome diversity compared to the commercial variety for all indexes. A core microbiome comprised of 158 taxa was present in all samples, while a greater number of unique bacterial taxa was recorded in the landrace samples compared to the commercial cultivar. Notably, landrace samples originated from organic farming had more than double the number of unique taxa compared to conventional counterparts. The study revealed a higher diversity of N<sub>2</sub> fixers and archaea, <i>Crenarchaeota</i> and <i>Thaumarchaeota</i>, in landrace samples and particularly in those derived from organic farming, underpinning the distinct recruiting efficiency of beneficial soil microbes by the landrace.https://www.mdpi.com/2073-4395/13/12/2910organic farmingconventional farmingnext-generation sequencingmetagenomicsmicrobial diversityN<sub>2</sub>-fixing bacteria |
| spellingShingle | Anthoula Gleridou Georgios Giannopoulos Alexios N. Polidoros Photini V. Mylona Lentil Landrace Seed Origin and Genotype Affects Rhizosphere Microbiome Agronomy organic farming conventional farming next-generation sequencing metagenomics microbial diversity N<sub>2</sub>-fixing bacteria |
| title | Lentil Landrace Seed Origin and Genotype Affects Rhizosphere Microbiome |
| title_full | Lentil Landrace Seed Origin and Genotype Affects Rhizosphere Microbiome |
| title_fullStr | Lentil Landrace Seed Origin and Genotype Affects Rhizosphere Microbiome |
| title_full_unstemmed | Lentil Landrace Seed Origin and Genotype Affects Rhizosphere Microbiome |
| title_short | Lentil Landrace Seed Origin and Genotype Affects Rhizosphere Microbiome |
| title_sort | lentil landrace seed origin and genotype affects rhizosphere microbiome |
| topic | organic farming conventional farming next-generation sequencing metagenomics microbial diversity N<sub>2</sub>-fixing bacteria |
| url | https://www.mdpi.com/2073-4395/13/12/2910 |
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