<i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public Health
<i>Aeromonas veronii</i> is an important pathogen causing freshwater fish sepsis and ulcer syndrome. An increasing number of cases have demonstrated its significance as an aquatic zoonotic agent. The purpose of this study was to ensure the safety of freshwater products by evaluating the...
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MDPI AG
2020-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/608 |
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| author | Tong Li Sayed Haidar Abbas Raza Bintong Yang Yufeng Sun Guiqin Wang Wuwen Sun Aidong Qian Chunfeng Wang Yuanhuan Kang Xiaofeng Shan |
| author_facet | Tong Li Sayed Haidar Abbas Raza Bintong Yang Yufeng Sun Guiqin Wang Wuwen Sun Aidong Qian Chunfeng Wang Yuanhuan Kang Xiaofeng Shan |
| author_sort | Tong Li |
| collection | DOAJ |
| description | <i>Aeromonas veronii</i> is an important pathogen causing freshwater fish sepsis and ulcer syndrome. An increasing number of cases have demonstrated its significance as an aquatic zoonotic agent. The purpose of this study was to ensure the safety of freshwater products by evaluating the infection status of edible freshwater fish. In this experiment, we isolated <i>A. veronii</i> from several species of apparently healthy freshwater fish, including <i>Carassius auratus</i>, <i>Cyprinus carpio</i>, <i>Ctenopharyngodon idella</i>, and <i>Silurus asotus</i>. <i>A. veronii</i> was identified through bacterial staining, culture characteristics, and 16S rDNA gene sequence. In addition, polymerase chain reaction (PCR) was used to investigate the distribution of seven major virulence genes, including aerolysin (<i>aer</i>: 88.51%), cytotoxic enterotoxin (<i>act</i>: 71.26%), serine proteinase (<i>ser</i>: 54.02%), adhesin (<i>Aha</i>: 40.23%), phospholipase (<i>lip</i>: 45.98%), nuclease (<i>exu</i>: 51.72%), and quorum sensing-controlled virulence factor (<i>LuxS</i>: 59.77%). In total, 496 strains of <i>Aeromonas</i> were isolated, including 87 strains of <i>A. veronii</i>. The isolates of <i>A. veronii</i> were Gram-negative, rod-shaped bacteria, and the colonies are yellow on Rimler-Shotts (RS) medium and showed greater than 99% homology with <i>A. veronii</i> ATCC35624 according to analyses of the 16S rDNA sequence. Nearly 50% of the <i>A. veronii</i> isolates carried at least four or more virulence genes, 25% of the isolates carried at least five types of virulence genes, and 59.77% isolates carried the <i>LuxS</i> gene, and the isolates carrying more virulence genes were found to be more virulent. These results are of great significance for further improving the food safety assessment of freshwater aquatic products. |
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| issn | 2076-2615 |
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| last_indexed | 2024-03-10T20:43:33Z |
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| spelling | doaj.art-1034e44959974df0a5f08511a314bcfd2023-11-19T20:29:50ZengMDPI AGAnimals2076-26152020-04-0110460810.3390/ani10040608<i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public HealthTong Li0Sayed Haidar Abbas Raza1Bintong Yang2Yufeng Sun3Guiqin Wang4Wuwen Sun5Aidong Qian6Chunfeng Wang7Yuanhuan Kang8Xiaofeng Shan9College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaCollege of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, China<i>Aeromonas veronii</i> is an important pathogen causing freshwater fish sepsis and ulcer syndrome. An increasing number of cases have demonstrated its significance as an aquatic zoonotic agent. The purpose of this study was to ensure the safety of freshwater products by evaluating the infection status of edible freshwater fish. In this experiment, we isolated <i>A. veronii</i> from several species of apparently healthy freshwater fish, including <i>Carassius auratus</i>, <i>Cyprinus carpio</i>, <i>Ctenopharyngodon idella</i>, and <i>Silurus asotus</i>. <i>A. veronii</i> was identified through bacterial staining, culture characteristics, and 16S rDNA gene sequence. In addition, polymerase chain reaction (PCR) was used to investigate the distribution of seven major virulence genes, including aerolysin (<i>aer</i>: 88.51%), cytotoxic enterotoxin (<i>act</i>: 71.26%), serine proteinase (<i>ser</i>: 54.02%), adhesin (<i>Aha</i>: 40.23%), phospholipase (<i>lip</i>: 45.98%), nuclease (<i>exu</i>: 51.72%), and quorum sensing-controlled virulence factor (<i>LuxS</i>: 59.77%). In total, 496 strains of <i>Aeromonas</i> were isolated, including 87 strains of <i>A. veronii</i>. The isolates of <i>A. veronii</i> were Gram-negative, rod-shaped bacteria, and the colonies are yellow on Rimler-Shotts (RS) medium and showed greater than 99% homology with <i>A. veronii</i> ATCC35624 according to analyses of the 16S rDNA sequence. Nearly 50% of the <i>A. veronii</i> isolates carried at least four or more virulence genes, 25% of the isolates carried at least five types of virulence genes, and 59.77% isolates carried the <i>LuxS</i> gene, and the isolates carrying more virulence genes were found to be more virulent. These results are of great significance for further improving the food safety assessment of freshwater aquatic products.https://www.mdpi.com/2076-2615/10/4/608<i>Aeromonas veronii</i>freshwater fishvirulence genespathogenicity test |
| spellingShingle | Tong Li Sayed Haidar Abbas Raza Bintong Yang Yufeng Sun Guiqin Wang Wuwen Sun Aidong Qian Chunfeng Wang Yuanhuan Kang Xiaofeng Shan <i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public Health Animals <i>Aeromonas veronii</i> freshwater fish virulence genes pathogenicity test |
| title | <i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public Health |
| title_full | <i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public Health |
| title_fullStr | <i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public Health |
| title_full_unstemmed | <i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public Health |
| title_short | <i>Aeromonas veronii</i> Infection in Commercial Freshwater Fish: A Potential Threat to Public Health |
| title_sort | i aeromonas veronii i infection in commercial freshwater fish a potential threat to public health |
| topic | <i>Aeromonas veronii</i> freshwater fish virulence genes pathogenicity test |
| url | https://www.mdpi.com/2076-2615/10/4/608 |
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