Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease
Background and Aim: A peptic ulcer is a breach in the gastric or duodenal mucosa down to the submucosa. There is evidence concerning the role of Reactive Oxygen Species (ROS) in the genesis of such ulcers production of intracellular ROS along mitochondria oxidative phosphorylation (OXPHOS) predisp...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Birjand University of Medical Sciences and Health Services
2014-05-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
| Subjects: | |
| Online Access: | http://journal.bums.ac.ir/browse.php?a_code=A-10-1206-1&slc_lang=en&sid=1 |
| _version_ | 1811244612973494272 |
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| author | Arezoo Haghighi Zivar Salehi Keyvan Aminian saba Fakhrieh Asl |
| author_facet | Arezoo Haghighi Zivar Salehi Keyvan Aminian saba Fakhrieh Asl |
| author_sort | Arezoo Haghighi |
| collection | DOAJ |
| description | Background and Aim: A peptic ulcer is a breach in the gastric or duodenal mucosa down to the submucosa. There is evidence concerning the role of Reactive Oxygen Species (ROS) in the genesis of such ulcers production of intracellular ROS along mitochondria oxidative phosphorylation (OXPHOS) predisposes the deletion of 4977 bp mtDNA.
The aim of the present study was to evaluate the association of 4977 bp mtDNA deletion with peptic ulcer disease.
Materials and Methods: In this case-control study included 110 patients with peptic ulcer disease and 110 healthy individuals were compared. Genomic DNAs of the cases and controls were extracted from bioptic tissues. Then, their genotypes were determined by means of Polymerase Chain Reaction (PCR). Finally, statistical analysis was performed using the MedCalc program.
Results: Deletion of 4977 bp mtDNA was found to be more frequent among patients with peptic ulcer disease (52.7%) compared to the controls (15.3%). A significant association was found between the deletion with peptic ulcer disease.
Conclusion: Deletion of 4977 bp in mitochondrial DNA is associated with peptic ulcer disease. |
| first_indexed | 2024-04-12T14:28:15Z |
| format | Article |
| id | doaj.art-1034fef6b98442f49970bc821cb5bc10 |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-04-12T14:28:15Z |
| publishDate | 2014-05-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-1034fef6b98442f49970bc821cb5bc102022-12-22T03:29:22ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522014-05-012114855Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer diseaseArezoo Haghighi0Zivar Salehi1Keyvan Aminian2saba Fakhrieh Asl3 Cellular and Molecular Sciences, Department of Biology, Faculty of Guilan Pardis, Department of Biology, University of Guilan, Rasht, Iran. Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran. Gastroenterologist, Internal Medicine Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran. Gastroenterologist, Internal Medicine Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran. Background and Aim: A peptic ulcer is a breach in the gastric or duodenal mucosa down to the submucosa. There is evidence concerning the role of Reactive Oxygen Species (ROS) in the genesis of such ulcers production of intracellular ROS along mitochondria oxidative phosphorylation (OXPHOS) predisposes the deletion of 4977 bp mtDNA. The aim of the present study was to evaluate the association of 4977 bp mtDNA deletion with peptic ulcer disease. Materials and Methods: In this case-control study included 110 patients with peptic ulcer disease and 110 healthy individuals were compared. Genomic DNAs of the cases and controls were extracted from bioptic tissues. Then, their genotypes were determined by means of Polymerase Chain Reaction (PCR). Finally, statistical analysis was performed using the MedCalc program. Results: Deletion of 4977 bp mtDNA was found to be more frequent among patients with peptic ulcer disease (52.7%) compared to the controls (15.3%). A significant association was found between the deletion with peptic ulcer disease. Conclusion: Deletion of 4977 bp in mitochondrial DNA is associated with peptic ulcer disease.http://journal.bums.ac.ir/browse.php?a_code=A-10-1206-1&slc_lang=en&sid=1Peptic ulcer disease Mitochondria genome |
| spellingShingle | Arezoo Haghighi Zivar Salehi Keyvan Aminian saba Fakhrieh Asl Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease مجله دانشگاه علوم پزشکی بیرجند Peptic ulcer disease Mitochondria genome |
| title | Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease |
| title_full | Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease |
| title_fullStr | Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease |
| title_full_unstemmed | Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease |
| title_short | Functional assessment of mitochondrial DNA 4977 bp deletion in peptic ulcer disease |
| title_sort | functional assessment of mitochondrial dna 4977 bp deletion in peptic ulcer disease |
| topic | Peptic ulcer disease Mitochondria genome |
| url | http://journal.bums.ac.ir/browse.php?a_code=A-10-1206-1&slc_lang=en&sid=1 |
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