SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European Genebanks
Around the world, there are more than 1500 genebanks storing plant genetic resources to be used in breeding and research. Such resources are essential for future food security, but many genebanks experience backlogs in their conservation work, often combined with limited budgets. Therefore, avoiding...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/8/925 |
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| author | Anna E. Palmé Jenny Hagenblad Svein Øivind Solberg Karolina Aloisi Anna Artemyeva |
| author_facet | Anna E. Palmé Jenny Hagenblad Svein Øivind Solberg Karolina Aloisi Anna Artemyeva |
| author_sort | Anna E. Palmé |
| collection | DOAJ |
| description | Around the world, there are more than 1500 genebanks storing plant genetic resources to be used in breeding and research. Such resources are essential for future food security, but many genebanks experience backlogs in their conservation work, often combined with limited budgets. Therefore, avoiding duplicate holdings is on the agenda. A process of coordination has started, aiming at sharing the responsibility of maintaining the unique accessions while allowing access according to the international treaty for plant genetic resources. Identifying duplicate holdings based on passport data has been one component of this. In the past, and especially in vegetables, different selections within the same varieties were common and the naming practices of cultivars/selections were flexible. Here, we examined 10 accession pairs/groups of cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) with similar names maintained in the Russian and Nordic genebanks. The accessions were analyzed for 11 morphological traits and with a SNP (Single Nucleotide Polymorphism) array developed for <i>B. napus</i>. Both proved to be useful tools for understanding the genetic structure among the accessions and for identifying duplicates, and a subset of 500 SNP markers are suggested for future <i>Brassica oleracea</i> genetic characterization. Within five out of 10 pairs/groups, we detected clear genetic differences among the accessions, and three of these were confirmed by significant differences in one or several morphological traits. In one case, a white cabbage and a red cabbage had similar accession names. The study highlights the necessity to be careful when identifying duplicate accessions based solely on the name, especially in older cross-pollinated species such as cabbage. |
| first_indexed | 2024-03-10T18:18:31Z |
| format | Article |
| id | doaj.art-1035212dfb2f468898851405d8e06b59 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:18:31Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1035212dfb2f468898851405d8e06b592023-11-20T07:31:14ZengMDPI AGPlants2223-77472020-07-019892510.3390/plants9080925SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European GenebanksAnna E. Palmé0Jenny Hagenblad1Svein Øivind Solberg2Karolina Aloisi3Anna Artemyeva4Nordic Genetic Resource Centre, Smedjevägen 3, SE-230 53 Alnarp, SwedenDepartment of Physics, Chemistry and Biology, Linköping University, SE-581 83 Linköping, SwedenFaculty of Applied Ecology, Agricultural Sciences and Biotechnology, Inland Norway University of Applied Sciences, NO-2418 Elverum, NorwayNordic Genetic Resource Centre, Smedjevägen 3, SE-230 53 Alnarp, SwedenN. I. Vavilov Institute of Plant Genetic Resources (VIR), 42-44, B. Morskaya Street, 190000 St. Petersburg, RussiaAround the world, there are more than 1500 genebanks storing plant genetic resources to be used in breeding and research. Such resources are essential for future food security, but many genebanks experience backlogs in their conservation work, often combined with limited budgets. Therefore, avoiding duplicate holdings is on the agenda. A process of coordination has started, aiming at sharing the responsibility of maintaining the unique accessions while allowing access according to the international treaty for plant genetic resources. Identifying duplicate holdings based on passport data has been one component of this. In the past, and especially in vegetables, different selections within the same varieties were common and the naming practices of cultivars/selections were flexible. Here, we examined 10 accession pairs/groups of cabbage (<i>Brassica oleracea</i> var. <i>capitata</i>) with similar names maintained in the Russian and Nordic genebanks. The accessions were analyzed for 11 morphological traits and with a SNP (Single Nucleotide Polymorphism) array developed for <i>B. napus</i>. Both proved to be useful tools for understanding the genetic structure among the accessions and for identifying duplicates, and a subset of 500 SNP markers are suggested for future <i>Brassica oleracea</i> genetic characterization. Within five out of 10 pairs/groups, we detected clear genetic differences among the accessions, and three of these were confirmed by significant differences in one or several morphological traits. In one case, a white cabbage and a red cabbage had similar accession names. The study highlights the necessity to be careful when identifying duplicate accessions based solely on the name, especially in older cross-pollinated species such as cabbage.https://www.mdpi.com/2223-7747/9/8/925<i>Brassica oleracea</i>conservationdiversitygenebankplant genetic resourcesSNP |
| spellingShingle | Anna E. Palmé Jenny Hagenblad Svein Øivind Solberg Karolina Aloisi Anna Artemyeva SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European Genebanks Plants <i>Brassica oleracea</i> conservation diversity genebank plant genetic resources SNP |
| title | SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European Genebanks |
| title_full | SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European Genebanks |
| title_fullStr | SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European Genebanks |
| title_full_unstemmed | SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European Genebanks |
| title_short | SNP Markers and Evaluation of Duplicate Holdings of <i>Brassica oleracea</i> in Two European Genebanks |
| title_sort | snp markers and evaluation of duplicate holdings of i brassica oleracea i in two european genebanks |
| topic | <i>Brassica oleracea</i> conservation diversity genebank plant genetic resources SNP |
| url | https://www.mdpi.com/2223-7747/9/8/925 |
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