Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers
There is a need for sesame (Sesamum indicum L.) breeding in Morocco to release performant and adapted varieties, which requires a large genetic variability in the germplasm to be used. In this context, genetic diversity of sesame populations from different locations in Tadla area was investigated us...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2021-01-01
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| Series: | Oilseeds and fats, crops and lipids |
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| Online Access: | https://www.ocl-journal.org/articles/ocl/full_html/2021/01/ocl200071/ocl200071.html |
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| author | El Harfi Meriem Charafi Jamal Houmanat Karim Hanine Hafida Nabloussi Abdelghani |
| author_facet | El Harfi Meriem Charafi Jamal Houmanat Karim Hanine Hafida Nabloussi Abdelghani |
| author_sort | El Harfi Meriem |
| collection | DOAJ |
| description | There is a need for sesame (Sesamum indicum L.) breeding in Morocco to release performant and adapted varieties, which requires a large genetic variability in the germplasm to be used. In this context, genetic diversity of sesame populations from different locations in Tadla area was investigated using ISSR (Inter Sequence Simple Repeats) markers as a simple method to reveal polymorphism among them. A total of 130 individuals representing 31 populations were sampled. Twenty-four ISSR primers were used for analysis of individuals representing the 31 different sesame populations grown in different agroclimatic zones of Tadla, accounting for 90% of sesame cultivation area in Morocco. Indeed, seven primers showed legible and reproducible genomic profiles with an interesting number of bands. A total of 57 bands were obtained with ISSR primers, of which 47 were polymorphic. PIC (Polymorphic Information Content) ranged from 0.002 to 0.350, showing that ISSR markers are informative and relevant for discriminating the populations evaluated. The similarity coefficient of ISSR data ranged from 0.509 to 1, with an average of 0.870. The results obtained showed that Moroccan sesame populations are characterized by a low genetic diversity, suggesting a genetic proximity among them. Therefore, new germplasm should be either introduced from diverse geographical origins or created through mutagenesis breeding in order to broaden the existing genetic variability. |
| first_indexed | 2024-12-14T12:06:24Z |
| format | Article |
| id | doaj.art-10386e0f2a464f63bece1460d9f9ae27 |
| institution | Directory Open Access Journal |
| issn | 2272-6977 2257-6614 |
| language | English |
| last_indexed | 2024-12-14T12:06:24Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Oilseeds and fats, crops and lipids |
| spelling | doaj.art-10386e0f2a464f63bece1460d9f9ae272022-12-21T23:01:51ZengEDP SciencesOilseeds and fats, crops and lipids2272-69772257-66142021-01-0128310.1051/ocl/2020072ocl200071Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markersEl Harfi MeriemCharafi Jamal0https://orcid.org/0000-0003-3301-177XHoumanat Karim1Hanine Hafida2https://orcid.org/0000-0002-9309-5672Nabloussi Abdelghani3https://orcid.org/0000-0002-6125-1490National Institute of Agronomic Research, Regional Agronomic Research CenterNational Institute of Agronomic Research, Regional Agronomic Research CenterDepartment of Biology, Faculty of Science and Technics, Sultan Moulay Slimane UniversityNational Institute of Agronomic Research, Regional Agronomic Research CenterThere is a need for sesame (Sesamum indicum L.) breeding in Morocco to release performant and adapted varieties, which requires a large genetic variability in the germplasm to be used. In this context, genetic diversity of sesame populations from different locations in Tadla area was investigated using ISSR (Inter Sequence Simple Repeats) markers as a simple method to reveal polymorphism among them. A total of 130 individuals representing 31 populations were sampled. Twenty-four ISSR primers were used for analysis of individuals representing the 31 different sesame populations grown in different agroclimatic zones of Tadla, accounting for 90% of sesame cultivation area in Morocco. Indeed, seven primers showed legible and reproducible genomic profiles with an interesting number of bands. A total of 57 bands were obtained with ISSR primers, of which 47 were polymorphic. PIC (Polymorphic Information Content) ranged from 0.002 to 0.350, showing that ISSR markers are informative and relevant for discriminating the populations evaluated. The similarity coefficient of ISSR data ranged from 0.509 to 1, with an average of 0.870. The results obtained showed that Moroccan sesame populations are characterized by a low genetic diversity, suggesting a genetic proximity among them. Therefore, new germplasm should be either introduced from diverse geographical origins or created through mutagenesis breeding in order to broaden the existing genetic variability.https://www.ocl-journal.org/articles/ocl/full_html/2021/01/ocl200071/ocl200071.htmlgenetic diversityissrs markersmoroccopolymorphismsesamum indicum l. |
| spellingShingle | El Harfi Meriem Charafi Jamal Houmanat Karim Hanine Hafida Nabloussi Abdelghani Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers Oilseeds and fats, crops and lipids genetic diversity issrs markers morocco polymorphism sesamum indicum l. |
| title | Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers |
| title_full | Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers |
| title_fullStr | Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers |
| title_full_unstemmed | Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers |
| title_short | Assessment of genetic diversity in Moroccan sesame (Sesamum indicum) using ISSR molecular markers |
| title_sort | assessment of genetic diversity in moroccan sesame sesamum indicum using issr molecular markers |
| topic | genetic diversity issrs markers morocco polymorphism sesamum indicum l. |
| url | https://www.ocl-journal.org/articles/ocl/full_html/2021/01/ocl200071/ocl200071.html |
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