Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19
Antiviral and inflammatory responses following the detection of the virus genome by nucleic acid sensors play a vital role in the pathogenesis and outcome of diseases. In this study, we investigated the ZBP1, AIM2, and MDA5 expression levels in COVID-19 patients with different intensities of the dis...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
2022-09-01
|
| Series: | EXCLI Journal : Experimental and Clinical Sciences |
| Subjects: | |
| Online Access: | https://www.excli.de/index.php/excli/article/view/5141 |
| _version_ | 1827963876169220096 |
|---|---|
| author | Nasir Arefinia Amin Ramezani Mehrdad Farokhnia Ali Mohammad Arab Zadeh Ramin Yaghobi Jamal Sarvari |
| author_facet | Nasir Arefinia Amin Ramezani Mehrdad Farokhnia Ali Mohammad Arab Zadeh Ramin Yaghobi Jamal Sarvari |
| author_sort | Nasir Arefinia |
| collection | DOAJ |
| description | Antiviral and inflammatory responses following the detection of the virus genome by nucleic acid sensors play a vital role in the pathogenesis and outcome of diseases. In this study, we investigated the ZBP1, AIM2, and MDA5 expression levels in COVID-19 patients with different intensities of the disease. 75 quantitative Real-Time PCR (qRT-PCR)-confirmed COVID-19 patients were included consecutively and divided into 3 groups of mild, severe, and critical based on the severity of the disease. Also, 25 healthy volunteer subjects were included. PBMCs were collected from the whole blood, and RNA was extracted using commercial kit. The expression of ZBP1, AIM2, and MDA5 genes was investigated using qRT-PCR technique. The mean age of the patients and healthy volunteers was 52.73±13.78 and 49.120±12.490, respectively. In each group, 13 out of 25 participants were male. The expression levels of ZBP1 (P=0.001), AIM2 (P=0.001), and MDA5 (P= 0.003) transcript were significantly higher in COVID-19 patients than the control group. The results also revealed that the expression levels of ZBP1, AIM2, and MDA5 were significantly higher in the critical and severe COVID-19 patients compared to those with mild disease (P<0.05). Moreover, regarding the gender, the expression levels of AIM2 and MDA5 were significantly elevated in male severe (P=0.04 and P=0.003, respectively) and critical (P=0.005 and P=0.0004, respectively) patients than the female ones. The results indicated that ZBP1, AIM2, and MDA5 genes might have an important role in the severity of COVID-19 disease. Moreover, the severity of COVID-19 disease in male and female patients might be related to AIM2, and MDA5 expression levels. More studies are recommended to be conducted to clarify this issue. |
| first_indexed | 2024-04-09T17:06:02Z |
| format | Article |
| id | doaj.art-103fa6e15d8846f3a2cc2c05dc7d9ea0 |
| institution | Directory Open Access Journal |
| issn | 1611-2156 |
| language | English |
| last_indexed | 2024-04-09T17:06:02Z |
| publishDate | 2022-09-01 |
| publisher | IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund |
| record_format | Article |
| series | EXCLI Journal : Experimental and Clinical Sciences |
| spelling | doaj.art-103fa6e15d8846f3a2cc2c05dc7d9ea02023-04-20T13:12:41ZengIfADo - Leibniz Research Centre for Working Environment and Human Factors, DortmundEXCLI Journal : Experimental and Clinical Sciences1611-21562022-09-01211171118310.17179/excli2022-51414580Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19Nasir Arefinia0https://orcid.org/0000-0002-7282-3538Amin Ramezani1https://orcid.org/0000-0001-5655-8722Mehrdad Farokhnia2https://orcid.org/0000-0002-0148-3628Ali Mohammad Arab Zadeh3Ramin Yaghobi4https://orcid.org/0000-0002-9812-8621Jamal Sarvari5https://orcid.org/0000-0002-2259-0836Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranShiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Internal Medicine, School of Medicine, Research Center for Hydatid Disease in Iran, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, IranDepartment of Internal Medicine, School of Medicine, Research Center for Hydatid Disease in Iran, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, IranTransplant Research Center, Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. Phone: +9871-42451791, Fax: +98 711 2304069, E-mail: rayaviro@yahoo.comDepartment of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Phone: +9871-42652781, FAX: +9871-42710780, Email: sarvarij@sums.ac.irAntiviral and inflammatory responses following the detection of the virus genome by nucleic acid sensors play a vital role in the pathogenesis and outcome of diseases. In this study, we investigated the ZBP1, AIM2, and MDA5 expression levels in COVID-19 patients with different intensities of the disease. 75 quantitative Real-Time PCR (qRT-PCR)-confirmed COVID-19 patients were included consecutively and divided into 3 groups of mild, severe, and critical based on the severity of the disease. Also, 25 healthy volunteer subjects were included. PBMCs were collected from the whole blood, and RNA was extracted using commercial kit. The expression of ZBP1, AIM2, and MDA5 genes was investigated using qRT-PCR technique. The mean age of the patients and healthy volunteers was 52.73±13.78 and 49.120±12.490, respectively. In each group, 13 out of 25 participants were male. The expression levels of ZBP1 (P=0.001), AIM2 (P=0.001), and MDA5 (P= 0.003) transcript were significantly higher in COVID-19 patients than the control group. The results also revealed that the expression levels of ZBP1, AIM2, and MDA5 were significantly higher in the critical and severe COVID-19 patients compared to those with mild disease (P<0.05). Moreover, regarding the gender, the expression levels of AIM2 and MDA5 were significantly elevated in male severe (P=0.04 and P=0.003, respectively) and critical (P=0.005 and P=0.0004, respectively) patients than the female ones. The results indicated that ZBP1, AIM2, and MDA5 genes might have an important role in the severity of COVID-19 disease. Moreover, the severity of COVID-19 disease in male and female patients might be related to AIM2, and MDA5 expression levels. More studies are recommended to be conducted to clarify this issue.https://www.excli.de/index.php/excli/article/view/5141zbp1aim2mda5sars-cov-2covid19severity of covid-19 |
| spellingShingle | Nasir Arefinia Amin Ramezani Mehrdad Farokhnia Ali Mohammad Arab Zadeh Ramin Yaghobi Jamal Sarvari Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19 EXCLI Journal : Experimental and Clinical Sciences zbp1 aim2 mda5 sars-cov-2 covid19 severity of covid-19 |
| title | Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19 |
| title_full | Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19 |
| title_fullStr | Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19 |
| title_full_unstemmed | Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19 |
| title_short | Association between expression of ZBP1, AIM2, and MDA5 genes and severity of COVID-19 |
| title_sort | association between expression of zbp1 aim2 and mda5 genes and severity of covid 19 |
| topic | zbp1 aim2 mda5 sars-cov-2 covid19 severity of covid-19 |
| url | https://www.excli.de/index.php/excli/article/view/5141 |
| work_keys_str_mv | AT nasirarefinia associationbetweenexpressionofzbp1aim2andmda5genesandseverityofcovid19 AT aminramezani associationbetweenexpressionofzbp1aim2andmda5genesandseverityofcovid19 AT mehrdadfarokhnia associationbetweenexpressionofzbp1aim2andmda5genesandseverityofcovid19 AT alimohammadarabzadeh associationbetweenexpressionofzbp1aim2andmda5genesandseverityofcovid19 AT raminyaghobi associationbetweenexpressionofzbp1aim2andmda5genesandseverityofcovid19 AT jamalsarvari associationbetweenexpressionofzbp1aim2andmda5genesandseverityofcovid19 |