PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS
The aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2016-08-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/1061 |
| _version_ | 1797197926968066048 |
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| author | A. A. Savchenko E. V. Kasparov S. S. Arutyunyan A. G. Borisov I. V. Kudryavtsev A. V. Moshev |
| author_facet | A. A. Savchenko E. V. Kasparov S. S. Arutyunyan A. G. Borisov I. V. Kudryavtsev A. V. Moshev |
| author_sort | A. A. Savchenko |
| collection | DOAJ |
| description | The aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight healthy agematched women participated as a control group. Phenotypic B-cell subpopulations were analyzed by flow cytometry performed with direct immunofluorescent staining of peripheral cells from whole blood using the following antibody panel: CD5-FITC/CD23-PE/CD19-ECD/CD45-PC5/CD27-PC7. A significantly reduced B-lymphocyte content was revealed in peripheral blood of women with chronic endometritis and adnexitis. The reduced cell numbers occurred due to reduced B2 (main fraction of B-lymphocytes) and as B1 cells (minor fraction) which determines insufficient reactivity of specific humoral immune response, including immune reactions at the mucous membranes. However, percentage of B2-lymphocytes was decreased only in endometriosis, whereas patients with adnexitis showed decrease in both relative and absolute counts of this B cell subpopulation. A decreased content of naive B-cells in the peripheral blood is another feature of the B cell phenotypic profile in chronic endometritis and adnexitis. Moreover, the drop of the naive B-cell levels in patients with adnexitis proved to be more pronounced than in persons with endometritis. Expression of CD23- antigen (a low-affinity receptor for IgE) has been investigated as a functional marker of B cells. All the studied peripheral B cell subpopulations expressing CD23 were decreased in the patients with chronic endometritis. The numbers of different B cell fractions expressing CD23 antigen were also reduced in the women with chronic adnexitis as compared to the levels detected in patients with chronic endometritis. Alterations of the B-cell immunity were more pronounced in chronic adnexitis, due to more extensive infectious/inflammatory process which involved both endometrium, and appendages, and clinical manifestation of the disease. The results suggest a need for evaluation of the B cell phenotypic profiles as a marker of inadequate immune response in infectious and inflammatory diseases of pelvic organs, as well as planning rational immunotherapy, in order to enhance therapeutic efficiency in these disorders. |
| first_indexed | 2024-03-08T05:49:21Z |
| format | Article |
| id | doaj.art-103fb3a169e44140a32afa066059f725 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:51:44Z |
| publishDate | 2016-08-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-103fb3a169e44140a32afa066059f7252024-04-22T13:07:41ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2016-08-0118437938410.15789/1563-0625-2016-4-379-384853PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITISA. A. Savchenko0E. V. Kasparov1S. S. Arutyunyan2A. G. Borisov3I. V. Kudryavtsev4A. V. Moshev5Research Institute of Medical Problems of the North; Krasnoyarsk V.F. Voino-Yasenetsky State Medical UniversityResearch Institute of Medical Problems of the NorthResearch Institute of Medical Problems of the NorthResearch Institute of Medical Problems of the North; Krasnoyarsk V.F. Voino-Yasenetsky State Medical UniversityResearch Institute of Experimental Medicine; Far Eastern Federal University; Pavlov First St. Petersburg State Medical UniversityResearch Institute of Medical Problems of the NorthThe aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight healthy agematched women participated as a control group. Phenotypic B-cell subpopulations were analyzed by flow cytometry performed with direct immunofluorescent staining of peripheral cells from whole blood using the following antibody panel: CD5-FITC/CD23-PE/CD19-ECD/CD45-PC5/CD27-PC7. A significantly reduced B-lymphocyte content was revealed in peripheral blood of women with chronic endometritis and adnexitis. The reduced cell numbers occurred due to reduced B2 (main fraction of B-lymphocytes) and as B1 cells (minor fraction) which determines insufficient reactivity of specific humoral immune response, including immune reactions at the mucous membranes. However, percentage of B2-lymphocytes was decreased only in endometriosis, whereas patients with adnexitis showed decrease in both relative and absolute counts of this B cell subpopulation. A decreased content of naive B-cells in the peripheral blood is another feature of the B cell phenotypic profile in chronic endometritis and adnexitis. Moreover, the drop of the naive B-cell levels in patients with adnexitis proved to be more pronounced than in persons with endometritis. Expression of CD23- antigen (a low-affinity receptor for IgE) has been investigated as a functional marker of B cells. All the studied peripheral B cell subpopulations expressing CD23 were decreased in the patients with chronic endometritis. The numbers of different B cell fractions expressing CD23 antigen were also reduced in the women with chronic adnexitis as compared to the levels detected in patients with chronic endometritis. Alterations of the B-cell immunity were more pronounced in chronic adnexitis, due to more extensive infectious/inflammatory process which involved both endometrium, and appendages, and clinical manifestation of the disease. The results suggest a need for evaluation of the B cell phenotypic profiles as a marker of inadequate immune response in infectious and inflammatory diseases of pelvic organs, as well as planning rational immunotherapy, in order to enhance therapeutic efficiency in these disorders.https://www.mimmun.ru/mimmun/article/view/1061b-lymphocyteschronic endometritischronic adnexitissubpopulation structureantibacterial immunitylow-affinity ige receptor |
| spellingShingle | A. A. Savchenko E. V. Kasparov S. S. Arutyunyan A. G. Borisov I. V. Kudryavtsev A. V. Moshev PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS Медицинская иммунология b-lymphocytes chronic endometritis chronic adnexitis subpopulation structure antibacterial immunity low-affinity ige receptor |
| title | PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS |
| title_full | PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS |
| title_fullStr | PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS |
| title_full_unstemmed | PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS |
| title_short | PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS |
| title_sort | phenotypic profile of b lymphocytes in women with chronic endometritis and adnexitis |
| topic | b-lymphocytes chronic endometritis chronic adnexitis subpopulation structure antibacterial immunity low-affinity ige receptor |
| url | https://www.mimmun.ru/mimmun/article/view/1061 |
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