A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia species

Artemisinin is a secondary metabolite with antimalarial properties. In addition to this, it has activities against a wide variety of cancers such as human leukemia, breast and colon cancer, and carcinomas. It has also shown anti hepatitis B, anti-HIV, anti-leishmanial and anti-schistosomiatic activi...

Full description

Bibliographic Details
Main Authors: Mona Moghadami, Ramin Hosseini, Kazem Yarizadeh
Format: Article
Language:fas
Published: University of Isfahan 2015-09-01
Series:Zīst/shināsī-i Giyāhī-i Īrān
Subjects:
Online Access:http://ijpb.ui.ac.ir/article_18972_f925af0368eb109ed99eeb721225c6da.pdf
_version_ 1831649238272966656
author Mona Moghadami
Ramin Hosseini
Kazem Yarizadeh
author_facet Mona Moghadami
Ramin Hosseini
Kazem Yarizadeh
author_sort Mona Moghadami
collection DOAJ
description Artemisinin is a secondary metabolite with antimalarial properties. In addition to this, it has activities against a wide variety of cancers such as human leukemia, breast and colon cancer, and carcinomas. It has also shown anti hepatitis B, anti-HIV, anti-leishmanial and anti-schistosomiatic activities. This compound is produced by many species of the genus Artemisia, and for the first time it was detected in A. annua. In this study, the expression patterns of ADS, DBR2 and SQS genes were investigated in five species include A. annua, A. dracunculus, A. vulgaris, A. absinthium, and A. sieberi. Extraction of total RNA and production of cDNA were from the two-month-old plant leaves. Semi-quantitative RT-PCR was performed to analyse the gene expression of these genes and actin gene was used as the internal control. Also, PCR products were loaded on the agarose gel (1 % w/v). Quantification of band density was carried out using Imag J software and data analysis was conducted using the SAS software. Results showed that expression pattern of the studied genes were different, so that a higher transcript level of SQS gene was observed in A. vulgaris, A. dracunculus and A. annua. Transcript level of ADS was higher than other genes in A. sieberi and A. absinthium. Different expression patterns of these genes can be interpreted as potentially different artemisinin contents in these species.
first_indexed 2024-12-19T14:46:56Z
format Article
id doaj.art-1044283f4fb44919a73571403facd99a
institution Directory Open Access Journal
issn 2008-8264
2322-2204
language fas
last_indexed 2024-12-19T14:46:56Z
publishDate 2015-09-01
publisher University of Isfahan
record_format Article
series Zīst/shināsī-i Giyāhī-i Īrān
spelling doaj.art-1044283f4fb44919a73571403facd99a2022-12-21T20:16:55ZfasUniversity of IsfahanZīst/shināsī-i Giyāhī-i Īrān2008-82642322-22042015-09-01725435218972A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia speciesMona Moghadami0Ramin Hosseini1Kazem Yarizadeh2Department of Agricultural Biotechnology, Faculty of Engineering and Technology, Imam Khomeini International University, Qazvin, IranDepartment of Agricultural Biotechnology, Faculty of Engineering and Technology, Imam Khomeini International University, Qazvin, IranDepartment of Agricultural Biotechnology, Faculty of Engineering and Technology, Imam Khomeini International University, Qazvin, IranArtemisinin is a secondary metabolite with antimalarial properties. In addition to this, it has activities against a wide variety of cancers such as human leukemia, breast and colon cancer, and carcinomas. It has also shown anti hepatitis B, anti-HIV, anti-leishmanial and anti-schistosomiatic activities. This compound is produced by many species of the genus Artemisia, and for the first time it was detected in A. annua. In this study, the expression patterns of ADS, DBR2 and SQS genes were investigated in five species include A. annua, A. dracunculus, A. vulgaris, A. absinthium, and A. sieberi. Extraction of total RNA and production of cDNA were from the two-month-old plant leaves. Semi-quantitative RT-PCR was performed to analyse the gene expression of these genes and actin gene was used as the internal control. Also, PCR products were loaded on the agarose gel (1 % w/v). Quantification of band density was carried out using Imag J software and data analysis was conducted using the SAS software. Results showed that expression pattern of the studied genes were different, so that a higher transcript level of SQS gene was observed in A. vulgaris, A. dracunculus and A. annua. Transcript level of ADS was higher than other genes in A. sieberi and A. absinthium. Different expression patterns of these genes can be interpreted as potentially different artemisinin contents in these species.http://ijpb.ui.ac.ir/article_18972_f925af0368eb109ed99eeb721225c6da.pdfAmorpha-4Artemisinin11-diene synthaseArtemisiaGene expressionAmorpha411diene synthaseSqualene synthase
spellingShingle Mona Moghadami
Ramin Hosseini
Kazem Yarizadeh
A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia species
Zīst/shināsī-i Giyāhī-i Īrān
Amorpha-4
Artemisinin
11-diene synthase
Artemisia
Gene expression
Amorpha
4
11
diene synthase
Squalene synthase
title A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia species
title_full A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia species
title_fullStr A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia species
title_full_unstemmed A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia species
title_short A survey on the expression of ADS, DBR2 and SQS genes in five Artemisia species
title_sort survey on the expression of ads dbr2 and sqs genes in five artemisia species
topic Amorpha-4
Artemisinin
11-diene synthase
Artemisia
Gene expression
Amorpha
4
11
diene synthase
Squalene synthase
url http://ijpb.ui.ac.ir/article_18972_f925af0368eb109ed99eeb721225c6da.pdf
work_keys_str_mv AT monamoghadami asurveyontheexpressionofadsdbr2andsqsgenesinfiveartemisiaspecies
AT raminhosseini asurveyontheexpressionofadsdbr2andsqsgenesinfiveartemisiaspecies
AT kazemyarizadeh asurveyontheexpressionofadsdbr2andsqsgenesinfiveartemisiaspecies
AT monamoghadami surveyontheexpressionofadsdbr2andsqsgenesinfiveartemisiaspecies
AT raminhosseini surveyontheexpressionofadsdbr2andsqsgenesinfiveartemisiaspecies
AT kazemyarizadeh surveyontheexpressionofadsdbr2andsqsgenesinfiveartemisiaspecies