miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit
Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discov...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/4/679 |
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| author | Fan Yao Bohao Zhao Shuaishuai Hu Shaocheng Bai Rongshuai Jin Chen Zhang Yang Chen Xinsheng Wu |
| author_facet | Fan Yao Bohao Zhao Shuaishuai Hu Shaocheng Bai Rongshuai Jin Chen Zhang Yang Chen Xinsheng Wu |
| author_sort | Fan Yao |
| collection | DOAJ |
| description | Mammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i>HOXC13</i> expression (Pearson’s R <i>=</i> −0.313, <i>p</i> < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i>HOXC13</i>. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i>BCL2</i>, <i>WNT2</i>, <i>CCND1,</i> and <i>LEF1</i> (<i>p</i> < 0.01), and promoted the expression of <i>SFRP2</i>, <i>TGF-β1,</i> and <i>FGF2</i> (<i>p</i> < 0.01), which was the same as the knockdown of <i>HOXC13</i>. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i>HOXC13</i>. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i>p</i> < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i>p</i> < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i>HOXC13,</i> and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans. |
| first_indexed | 2024-03-09T10:34:46Z |
| format | Article |
| id | doaj.art-1044b253bb944d54bd875338d832ba43 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T10:34:46Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-1044b253bb944d54bd875338d832ba432023-12-01T20:57:27ZengMDPI AGGenes2073-44252022-04-0113467910.3390/genes13040679miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in RabbitFan Yao0Bohao Zhao1Shuaishuai Hu2Shaocheng Bai3Rongshuai Jin4Chen Zhang5Yang Chen6Xinsheng Wu7College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaMammalian hair formation is critically determined by the growth of hair follicles (HF). MiRNAs are crucial in the periodic development of hair follicles; they maintain epidermal homeostasis by targeting genes and influencing the activity of signaling pathways and related regulators. Our study discovered miR-129-5p to be overexpressed in the skin of Angora rabbits during catagen, and was negatively correlated with <i>HOXC13</i> expression (Pearson’s R <i>=</i> −0.313, <i>p</i> < 0.05). The dual-Luciferase reporter gene detection system and Western blotting confirmed that miR-129-5p targeted <i>HOXC13</i>. In addition, miR-129-5p overexpression was found to significantly inhibit the expression of hair follicle development-related genes (HFDRGs), such as <i>BCL2</i>, <i>WNT2</i>, <i>CCND1,</i> and <i>LEF1</i> (<i>p</i> < 0.01), and promoted the expression of <i>SFRP2</i>, <i>TGF-β1,</i> and <i>FGF2</i> (<i>p</i> < 0.01), which was the same as the knockdown of <i>HOXC13</i>. In contrast, the knockout of miR-129-5p was the opposite, and it demonstrated similar results to the overexpression of <i>HOXC13</i>. CCK8 and flow cytometry demonstrated that miR-129-5p mimics significantly promoted the apoptosis of dermal papilla cells (DPCs) and inhibited proliferation (<i>p</i> < 0.01), while the inhibitor was found to reduce the apoptosis of DPCs and promote proliferation (<i>p</i> < 0.01). These results showed that miR-129-5p can participate in the periodic development of HF by targeting <i>HOXC13,</i> and it can induce apoptosis and inhibit proliferation of DPCs. These results will help to understand the role and mechanism of miR-129-5p in the periodic development of HF, and will provide support for subsequent studies, not only providing a theoretical basis for genetically improving the quality of hair in animals in the future, but also a new theory and method for diagnosing and treating hair loss in humans.https://www.mdpi.com/2073-4425/13/4/679miR-129-5p<i>HOXC13</i>DPCsproliferation and apoptosis |
| spellingShingle | Fan Yao Bohao Zhao Shuaishuai Hu Shaocheng Bai Rongshuai Jin Chen Zhang Yang Chen Xinsheng Wu miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit Genes miR-129-5p <i>HOXC13</i> DPCs proliferation and apoptosis |
| title | miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit |
| title_full | miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit |
| title_fullStr | miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit |
| title_full_unstemmed | miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit |
| title_short | miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit |
| title_sort | mir 129 5p participates in hair follicle growth by targeting hoxc13 in rabbit |
| topic | miR-129-5p <i>HOXC13</i> DPCs proliferation and apoptosis |
| url | https://www.mdpi.com/2073-4425/13/4/679 |
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