Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro−transfection

Establishment of targeted mutagenesis in soybean protoplasts using CRISPR/Cas9 RNP delivery via electro−transfection

The soybean (Glycine max L.) is an important crop with high agronomic value. The improvement of agronomic traits through gene editing techniques has broad application prospects in soybean. The polyethylene glycol (PEG)-mediated cell transfection has been successfully used to deliver the CRISPR/Cas9-...

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Bibliographic Details
Main Authors: Saminathan Subburaj, Sarah Zanon Agapito-Tenfen
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-09-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9 RNPs
gene-editing
neon electroporation system
soybean
protoplasts
target-deep sequencing
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2023.1255819/full

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