Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)

The need of new food sources to satisfy human requirements forces researchers to study any possible alternative supplies. Therefore this study aimed to explore the quality and digestibility evaluation of the protein fractions obtained as a by-product of processing the shrimp exoskeleton Litopen...

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Main Authors: Escobedo-Lozano, A.Y., Méndez Gómez, E., Vázquez-Olivares, A.E., Martínez-Sánchez, H.F
Format: Article
Language:English
Published: Universidad Autónoma de Nayarit 2014-01-01
Series:Revista Bio Ciencias
Subjects:
Online Access:http://biociencias.uan.edu.mx/publicaciones/04-04/biociencias4-4-7.pdf
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author Escobedo-Lozano, A.Y.
Méndez Gómez, E.
Vázquez-Olivares, A.E.
Martínez-Sánchez, H.F
author_facet Escobedo-Lozano, A.Y.
Méndez Gómez, E.
Vázquez-Olivares, A.E.
Martínez-Sánchez, H.F
author_sort Escobedo-Lozano, A.Y.
collection DOAJ
description The need of new food sources to satisfy human requirements forces researchers to study any possible alternative supplies. Therefore this study aimed to explore the quality and digestibility evaluation of the protein fractions obtained as a by-product of processing the shrimp exoskeleton Litopenaeus vannamei. Shrimp exoskeletons were washed, they were dried, mincedand they were brought under acid hydrolysis and alkaline hydrolysis; released proteins were then precipitated and characterized. 496 g of demineralized exoskeleton were obtained from 1 kg of shrimp shell; out of these, 376 g corresponded to chitin and 120 g corresponded to protein Total protein content was 33.80 ± 0.34 %. The digestible protein fractions were 26.7 g and 92.1 g were insoluble proteins. Of this latter fraction, the largest proportion belonged to the scleroprotein type. The amino acid profile analysis from the recovered proteins indicated that there were present nine amino acids out of the ten essential ones, being leucine the one with the highest proportion. In vitro digestibility was shown to be up to an 83.7 %. Therefore, it was possible to conclude that the recovery of digestibleproteins from shrimp exoskeleton may be useful in diet formulation.
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spelling doaj.art-104f89f508d841f39a3fd816bff9a4432022-12-21T19:19:59ZengUniversidad Autónoma de NayaritRevista Bio Ciencias2007-33802007-33802014-01-0124293301Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)Escobedo-Lozano, A.Y.0Méndez Gómez, E.1Vázquez-Olivares, A.E.2Martínez-Sánchez, H.F3Instituto Tecnológico de Mazatlán, Departamento de Ingeniería Química y Bioquímica. México.Instituto Tecnológico de Mazatlán, Departamento de Ingeniería Química y Bioquímica. México.Instituto Tecnológico de Mazatlán, Departamento de Ingeniería Química y Bioquímica. México.Instituto Tecnológico de Mazatlán, Departamento de Ingeniería Química y Bioquímica. México.The need of new food sources to satisfy human requirements forces researchers to study any possible alternative supplies. Therefore this study aimed to explore the quality and digestibility evaluation of the protein fractions obtained as a by-product of processing the shrimp exoskeleton Litopenaeus vannamei. Shrimp exoskeletons were washed, they were dried, mincedand they were brought under acid hydrolysis and alkaline hydrolysis; released proteins were then precipitated and characterized. 496 g of demineralized exoskeleton were obtained from 1 kg of shrimp shell; out of these, 376 g corresponded to chitin and 120 g corresponded to protein Total protein content was 33.80 ± 0.34 %. The digestible protein fractions were 26.7 g and 92.1 g were insoluble proteins. Of this latter fraction, the largest proportion belonged to the scleroprotein type. The amino acid profile analysis from the recovered proteins indicated that there were present nine amino acids out of the ten essential ones, being leucine the one with the highest proportion. In vitro digestibility was shown to be up to an 83.7 %. Therefore, it was possible to conclude that the recovery of digestibleproteins from shrimp exoskeleton may be useful in diet formulation.http://biociencias.uan.edu.mx/publicaciones/04-04/biociencias4-4-7.pdfProteinsexoskeletonshrimpdigestibilityfood.
spellingShingle Escobedo-Lozano, A.Y.
Méndez Gómez, E.
Vázquez-Olivares, A.E.
Martínez-Sánchez, H.F
Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)
Revista Bio Ciencias
Proteins
exoskeleton
shrimp
digestibility
food.
title Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)
title_full Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)
title_fullStr Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)
title_full_unstemmed Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)
title_short Extraction, partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp (Litopenaeus vannamei)
title_sort extraction partial characterization and evaluation of in vitro digestibility of the protein associated with the exoskeleton of white shrimp litopenaeus vannamei
topic Proteins
exoskeleton
shrimp
digestibility
food.
url http://biociencias.uan.edu.mx/publicaciones/04-04/biociencias4-4-7.pdf
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