Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq
Aim: Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq. Materials and Methods: A total of 120 black rats (R. rattus) were trapped from different areas in Baghdad city. Rats were k...
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| Format: | Article |
| Language: | English |
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Veterinary World
2019-01-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.12/January-2019/17.pdf |
| _version_ | 1818622000472522752 |
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| author | Nagham Mohammed Ayyal Zainab Abdulzahra Abbas Abdulkarim Jafar Karim Zainab Majid Abbas Karima Akool Al-Salihi Jenan Mahmood Khalaf Dunya Dhafir Mahmood Eman Abdullah Mohammed Rawaa Saladdin Jumaa Dhuha Ismaeel Abdul-Majeed |
| author_facet | Nagham Mohammed Ayyal Zainab Abdulzahra Abbas Abdulkarim Jafar Karim Zainab Majid Abbas Karima Akool Al-Salihi Jenan Mahmood Khalaf Dunya Dhafir Mahmood Eman Abdullah Mohammed Rawaa Saladdin Jumaa Dhuha Ismaeel Abdul-Majeed |
| author_sort | Nagham Mohammed Ayyal |
| collection | DOAJ |
| description | Aim: Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq.
Materials and Methods: A total of 120 black rats (R. rattus) were trapped from different areas in Baghdad city. Rats were kept in individual plastic cages for 3 h before euthanizing. Deep pharyngeal swab, intestinal content, urine, and pieces of the liver and spleen, lung, kidney, and brain were obtained aseptically. The specimens were inoculated into peptone water and incubated at 37°C for 24 h for enrichment. A loopful of each specimen was then subcultured onto MacConkey Agar, Blood Agar, and Mannitol Salt Agar. CHROMagar O157 H7 and CHROMagar Listeria were used to detect Escherichia coli 157:7 and Listeria spp., respectively. Biochemical tests on analytical profile index, microscopic examination, and commercial kit for latex agglutination test for serotyping E. coli O157:H7 were used.
Results: Mixed bacterial isolates were recorded as 116, 52, 36, 28, 18, 6, and 4 from intestinal contents, deep pharyngeal, liver and spleen, urine, lung, brain, and kidney, respectively. Microorganisms included E. coli, Staphylococcus aureus, Streptococcus spp., Bacillus spp., Pseudomonas aeruginosa, Citrobacter freundii, Proteus vulgaris, E. coli O157:H7, Enterobacter cloacae, Listeria spp., Klebsiella spp., Ochrobactrum anthropi, Aeromonas spp., Brucella spp., Pseudomonas fluorescens, Escherichia fergusonii, Micrococcus spp., Morganella spp., Proteus mirabilis, Pseudomonas luteola, and Streptobacillus spp. The highest bacterial prevalence (88; 73.33%) was recorded for E. coli, where 68 isolates were identified from the intestinal contents. Of these, four isolates were E. coli O157:H7.
Conclusion: Rats are important carriers and transmitters of a number of pathogens and can disseminate these microorganisms to humans and animals. |
| first_indexed | 2024-12-16T18:18:13Z |
| format | Article |
| id | doaj.art-1050035ec6cc4abdab4e0bbef6ae6d11 |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T18:18:13Z |
| publishDate | 2019-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-1050035ec6cc4abdab4e0bbef6ae6d112022-12-21T22:21:38ZengVeterinary WorldVeterinary World0972-89882231-09162019-01-0112111912510.14202/vetworld.2019.119-125Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of IraqNagham Mohammed Ayyal0Zainab Abdulzahra Abbas1Abdulkarim Jafar Karim2Zainab Majid Abbas3Karima Akool Al-Salihi4Jenan Mahmood Khalaf5Dunya Dhafir Mahmood6Eman Abdullah Mohammed7Rawaa Saladdin Jumaa8Dhuha Ismaeel Abdul-Majeed9Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Department of Pathological Analysis, Babylon Technical Institute, Al-Furat Al-Awsat Technical University, Babylon, Iraq.Department of Internal and Preventive Medicine, College of Veterinary Medicine, Al-Muthanna University, Al-Muthanna, Iraq.Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.Aim: Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq. Materials and Methods: A total of 120 black rats (R. rattus) were trapped from different areas in Baghdad city. Rats were kept in individual plastic cages for 3 h before euthanizing. Deep pharyngeal swab, intestinal content, urine, and pieces of the liver and spleen, lung, kidney, and brain were obtained aseptically. The specimens were inoculated into peptone water and incubated at 37°C for 24 h for enrichment. A loopful of each specimen was then subcultured onto MacConkey Agar, Blood Agar, and Mannitol Salt Agar. CHROMagar O157 H7 and CHROMagar Listeria were used to detect Escherichia coli 157:7 and Listeria spp., respectively. Biochemical tests on analytical profile index, microscopic examination, and commercial kit for latex agglutination test for serotyping E. coli O157:H7 were used. Results: Mixed bacterial isolates were recorded as 116, 52, 36, 28, 18, 6, and 4 from intestinal contents, deep pharyngeal, liver and spleen, urine, lung, brain, and kidney, respectively. Microorganisms included E. coli, Staphylococcus aureus, Streptococcus spp., Bacillus spp., Pseudomonas aeruginosa, Citrobacter freundii, Proteus vulgaris, E. coli O157:H7, Enterobacter cloacae, Listeria spp., Klebsiella spp., Ochrobactrum anthropi, Aeromonas spp., Brucella spp., Pseudomonas fluorescens, Escherichia fergusonii, Micrococcus spp., Morganella spp., Proteus mirabilis, Pseudomonas luteola, and Streptobacillus spp. The highest bacterial prevalence (88; 73.33%) was recorded for E. coli, where 68 isolates were identified from the intestinal contents. Of these, four isolates were E. coli O157:H7. Conclusion: Rats are important carriers and transmitters of a number of pathogens and can disseminate these microorganisms to humans and animals.http://www.veterinaryworld.org/Vol.12/January-2019/17.pdfbacteriadifferent organsEscherichia coli O157:H7Pseudomonas aeruginosaraturine |
| spellingShingle | Nagham Mohammed Ayyal Zainab Abdulzahra Abbas Abdulkarim Jafar Karim Zainab Majid Abbas Karima Akool Al-Salihi Jenan Mahmood Khalaf Dunya Dhafir Mahmood Eman Abdullah Mohammed Rawaa Saladdin Jumaa Dhuha Ismaeel Abdul-Majeed Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq Veterinary World bacteria different organs Escherichia coli O157:H7 Pseudomonas aeruginosa rat urine |
| title | Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq |
| title_full | Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq |
| title_fullStr | Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq |
| title_full_unstemmed | Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq |
| title_short | Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq |
| title_sort | bacterial isolation from internal organs of rats rattus rattus captured in baghdad city of iraq |
| topic | bacteria different organs Escherichia coli O157:H7 Pseudomonas aeruginosa rat urine |
| url | http://www.veterinaryworld.org/Vol.12/January-2019/17.pdf |
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