Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania
This cross-sectional study was conducted between January and June 2020, in five large poultry slaughter slabs in Dar es Salaam, Tanzania. Purposive sampling was used to select broilers and spent layers, from which meat and cloaca swabs were collected to determine the occurrence of multidrug resistan...
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MDPI AG
2021-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/4/343 |
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| author | Fauster X. Mgaya Mecky I. Matee Amandus P. Muhairwa Abubakar S. Hoza |
| author_facet | Fauster X. Mgaya Mecky I. Matee Amandus P. Muhairwa Abubakar S. Hoza |
| author_sort | Fauster X. Mgaya |
| collection | DOAJ |
| description | This cross-sectional study was conducted between January and June 2020, in five large poultry slaughter slabs in Dar es Salaam, Tanzania. Purposive sampling was used to select broilers and spent layers, from which meat and cloaca swabs were collected to determine the occurrence of multidrug resistant (MDR) <i>Escherichia coli</i>. Identification of isolates was done using API 20E, and antimicrobial susceptibility testing was performed as per CLSI (2018) guidelines. EBSL (<i>CTX-M</i>, TEM, SHV) and plasmid mediated quinolone (<i>qnrA</i>, <i>qnrB</i>, <i>qnrS</i> and <i>aac(6′)-Ib-cr</i>) were screened using PCR. Out of 384 samples, 212 (55.2%) were positive for <i>E. coli</i>, of which 147 (69.3%) were resistant to multiple drugs (MDR). Highest resistance was detected to tetracycline (91.9%), followed by sulfamethoxazole-trimethoprim (80.5%), ampicillin (70.9%), ciprofloxacin (40.2%) and 25% cefotaxime, gentamycin (10.8%) and imipenem (8.6%) (95% CI, <i>p</i> < 0.01). Out of the <i>E. coli</i>-positive samples, ten (10/212) (4.7%) were ESBL producing <i>E. coli,</i> of which CTX-M was detected in two isolates and quinolones resistant gene (<i>qnrS)</i> in eight, while TEM, SHV, <i>qnrA</i>, <i>qnrB</i> and <i>aac(6′)-lb-cr</i> were not detected. The high level of resistance and multidrug resistance imply these antibiotics are ineffective, add unnecessary cost to poultry farmers and certainly facilitate emergence and spread of resistance. |
| first_indexed | 2024-03-10T12:57:44Z |
| format | Article |
| id | doaj.art-10506179c70549f3b42a205e8fcabc6b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T12:57:44Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-10506179c70549f3b42a205e8fcabc6b2023-11-21T11:45:18ZengMDPI AGAntibiotics2079-63822021-03-0110434310.3390/antibiotics10040343Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, TanzaniaFauster X. Mgaya0Mecky I. Matee1Amandus P. Muhairwa2Abubakar S. Hoza3Department of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, Morogoro 67000, TanzaniaDepartment of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Dar es Salaam 65001, TanzaniaDepartment of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Morogoro 67000, TanzaniaDepartment of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, Morogoro 67000, TanzaniaThis cross-sectional study was conducted between January and June 2020, in five large poultry slaughter slabs in Dar es Salaam, Tanzania. Purposive sampling was used to select broilers and spent layers, from which meat and cloaca swabs were collected to determine the occurrence of multidrug resistant (MDR) <i>Escherichia coli</i>. Identification of isolates was done using API 20E, and antimicrobial susceptibility testing was performed as per CLSI (2018) guidelines. EBSL (<i>CTX-M</i>, TEM, SHV) and plasmid mediated quinolone (<i>qnrA</i>, <i>qnrB</i>, <i>qnrS</i> and <i>aac(6′)-Ib-cr</i>) were screened using PCR. Out of 384 samples, 212 (55.2%) were positive for <i>E. coli</i>, of which 147 (69.3%) were resistant to multiple drugs (MDR). Highest resistance was detected to tetracycline (91.9%), followed by sulfamethoxazole-trimethoprim (80.5%), ampicillin (70.9%), ciprofloxacin (40.2%) and 25% cefotaxime, gentamycin (10.8%) and imipenem (8.6%) (95% CI, <i>p</i> < 0.01). Out of the <i>E. coli</i>-positive samples, ten (10/212) (4.7%) were ESBL producing <i>E. coli,</i> of which CTX-M was detected in two isolates and quinolones resistant gene (<i>qnrS)</i> in eight, while TEM, SHV, <i>qnrA</i>, <i>qnrB</i> and <i>aac(6′)-lb-cr</i> were not detected. The high level of resistance and multidrug resistance imply these antibiotics are ineffective, add unnecessary cost to poultry farmers and certainly facilitate emergence and spread of resistance.https://www.mdpi.com/2079-6382/10/4/343multidrug resistant<i>Escherichia coli</i>chicken meatcloacaDar es SalaamTanzania |
| spellingShingle | Fauster X. Mgaya Mecky I. Matee Amandus P. Muhairwa Abubakar S. Hoza Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania Antibiotics multidrug resistant <i>Escherichia coli</i> chicken meat cloaca Dar es Salaam Tanzania |
| title | Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania |
| title_full | Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania |
| title_fullStr | Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania |
| title_full_unstemmed | Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania |
| title_short | Occurrence of Multidrug Resistant <i>Escherichia coli</i> in Raw Meat and Cloaca Swabs in Poultry Processed in Slaughter Slabs in Dar es Salaam, Tanzania |
| title_sort | occurrence of multidrug resistant i escherichia coli i in raw meat and cloaca swabs in poultry processed in slaughter slabs in dar es salaam tanzania |
| topic | multidrug resistant <i>Escherichia coli</i> chicken meat cloaca Dar es Salaam Tanzania |
| url | https://www.mdpi.com/2079-6382/10/4/343 |
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