Automatic cell identification and counting of leaf epidermis for plant phenotyping
The counting of leaf epidermal cells is useful to study the plant developmental changes produced by environmental or genetic factors.The scanning electron microscopy can be used, but it is expensive and time-consuming. Methods using optical microscopy are also available, but they still require leave...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-01-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016120300790 |
| _version_ | 1818325342448779264 |
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| author | Giacomo Mele Laura Gargiulo |
| author_facet | Giacomo Mele Laura Gargiulo |
| author_sort | Giacomo Mele |
| collection | DOAJ |
| description | The counting of leaf epidermal cells is useful to study the plant developmental changes produced by environmental or genetic factors.The scanning electron microscopy can be used, but it is expensive and time-consuming. Methods using optical microscopy are also available, but they still require leaves pre-treatment and manual cell identification.We propose a quick and simple method for counting leaf epidermal cells without leaf treatments and based on automated cell identification and marking. It allows to highly improve the representativeness of leaf epidermis screening, aiming at a high-throughput plant phenotyping approach. • The leaves are pressed between two glass slides without any pre-treatment and digital micrographs are acquired under incident light. • Epidermal cells are automatically identified and counted by means of a “macro'' of ImageJ • The cell count obtained applying the procedure of image processing is very close to that obtainable by manual cell identification. |
| first_indexed | 2024-12-13T11:42:57Z |
| format | Article |
| id | doaj.art-10528b053fa74bd692ea5c31a3d494c8 |
| institution | Directory Open Access Journal |
| issn | 2215-0161 |
| language | English |
| last_indexed | 2024-12-13T11:42:57Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj.art-10528b053fa74bd692ea5c31a3d494c82022-12-21T23:47:34ZengElsevierMethodsX2215-01612020-01-017100860Automatic cell identification and counting of leaf epidermis for plant phenotypingGiacomo Mele0Laura Gargiulo1Institute for Agricultural and Forest Systems in the Mediterranean - National Research Council, ISAFOM-CNR, Via Patacca 85, 80056 Ercolano, ItalyCorresponding author.; Institute for Agricultural and Forest Systems in the Mediterranean - National Research Council, ISAFOM-CNR, Via Patacca 85, 80056 Ercolano, ItalyThe counting of leaf epidermal cells is useful to study the plant developmental changes produced by environmental or genetic factors.The scanning electron microscopy can be used, but it is expensive and time-consuming. Methods using optical microscopy are also available, but they still require leaves pre-treatment and manual cell identification.We propose a quick and simple method for counting leaf epidermal cells without leaf treatments and based on automated cell identification and marking. It allows to highly improve the representativeness of leaf epidermis screening, aiming at a high-throughput plant phenotyping approach. • The leaves are pressed between two glass slides without any pre-treatment and digital micrographs are acquired under incident light. • Epidermal cells are automatically identified and counted by means of a “macro'' of ImageJ • The cell count obtained applying the procedure of image processing is very close to that obtainable by manual cell identification.http://www.sciencedirect.com/science/article/pii/S2215016120300790Leaf image analysisLeaf optical microscopyImageJ |
| spellingShingle | Giacomo Mele Laura Gargiulo Automatic cell identification and counting of leaf epidermis for plant phenotyping MethodsX Leaf image analysis Leaf optical microscopy ImageJ |
| title | Automatic cell identification and counting of leaf epidermis for plant phenotyping |
| title_full | Automatic cell identification and counting of leaf epidermis for plant phenotyping |
| title_fullStr | Automatic cell identification and counting of leaf epidermis for plant phenotyping |
| title_full_unstemmed | Automatic cell identification and counting of leaf epidermis for plant phenotyping |
| title_short | Automatic cell identification and counting of leaf epidermis for plant phenotyping |
| title_sort | automatic cell identification and counting of leaf epidermis for plant phenotyping |
| topic | Leaf image analysis Leaf optical microscopy ImageJ |
| url | http://www.sciencedirect.com/science/article/pii/S2215016120300790 |
| work_keys_str_mv | AT giacomomele automaticcellidentificationandcountingofleafepidermisforplantphenotyping AT lauragargiulo automaticcellidentificationandcountingofleafepidermisforplantphenotyping |