Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging
Microcalcifications are detected through mammography screening and, depending on their morphology and distribution (BI-RADS classification), they can be considered one of the first indicators of suspicious cancer lesions. However, the formation of hydroxyapatite (HAp) calcifications and their relati...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/13/11/2658 |
| _version_ | 1797532236572000256 |
|---|---|
| author | Monica Marro Anna M. Rodríguez-Rivero Cuauhtémoc Araujo-Andrade Maria Teresa Fernández-Figueras Laia Pérez-Roca Eva Castellà Jordi Navinés Antonio Mariscal Joan Francesc Julián Pau Turon Pablo Loza-Alvarez |
| author_facet | Monica Marro Anna M. Rodríguez-Rivero Cuauhtémoc Araujo-Andrade Maria Teresa Fernández-Figueras Laia Pérez-Roca Eva Castellà Jordi Navinés Antonio Mariscal Joan Francesc Julián Pau Turon Pablo Loza-Alvarez |
| author_sort | Monica Marro |
| collection | DOAJ |
| description | Microcalcifications are detected through mammography screening and, depending on their morphology and distribution (BI-RADS classification), they can be considered one of the first indicators of suspicious cancer lesions. However, the formation of hydroxyapatite (HAp) calcifications and their relationship with malignancy remains unknown. In this work, we report the most detailed three-dimensional biochemical analysis of breast cancer microcalcifications to date, combining 3D Raman spectroscopy imaging and advanced multivariate analysis in order to investigate in depth the molecular composition of HAp calcifications found in 26 breast cancer tissue biopsies. We demonstrate that DNA has been naturally adsorbed and encapsulated inside HAp microcalcifications. Furthermore, we also show the encapsulation of other relevant biomolecules in HAp calcifications, such as lipids, proteins, cytochrome C and polysaccharides. The demonstration of natural DNA biomineralization, particularly in the tumor microenvironment, represents an unprecedented advance in the field, as it can pave the way to understanding the role of HAp in malignant tissues. |
| first_indexed | 2024-03-10T10:56:14Z |
| format | Article |
| id | doaj.art-1052d573b2484432ab23cbe2b428b6af |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T10:56:14Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-1052d573b2484432ab23cbe2b428b6af2023-11-21T21:49:27ZengMDPI AGCancers2072-66942021-05-011311265810.3390/cancers13112658Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman ImagingMonica Marro0Anna M. Rodríguez-Rivero1Cuauhtémoc Araujo-Andrade2Maria Teresa Fernández-Figueras3Laia Pérez-Roca4Eva Castellà5Jordi Navinés6Antonio Mariscal7Joan Francesc Julián8Pau Turon9Pablo Loza-Alvarez10ICFO—Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, 08860 Barcelona, SpainResearch and Development B. Braun Surgical, S.A.U. Ctra. de Terrassa, 121, Rubí, 08191 Barcelona, SpainICFO—Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, 08860 Barcelona, SpainDepartament de Medicina, Facultat de Medicina i Ciències de la Salut, Universitat Internacional de Catalunya, Carrer de la Immaculada, 22, 08017 Barcelona, SpainInstitut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, SpainInstitut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, SpainInstitut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, SpainInstitut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, SpainCampus de la UAB, Universitat Autònoma de Barcelona, Plaça Cívica, Bellaterra, 08193 Barcelona, SpainResearch and Development B. Braun Surgical, S.A.U. Ctra. de Terrassa, 121, Rubí, 08191 Barcelona, SpainICFO—Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, 08860 Barcelona, SpainMicrocalcifications are detected through mammography screening and, depending on their morphology and distribution (BI-RADS classification), they can be considered one of the first indicators of suspicious cancer lesions. However, the formation of hydroxyapatite (HAp) calcifications and their relationship with malignancy remains unknown. In this work, we report the most detailed three-dimensional biochemical analysis of breast cancer microcalcifications to date, combining 3D Raman spectroscopy imaging and advanced multivariate analysis in order to investigate in depth the molecular composition of HAp calcifications found in 26 breast cancer tissue biopsies. We demonstrate that DNA has been naturally adsorbed and encapsulated inside HAp microcalcifications. Furthermore, we also show the encapsulation of other relevant biomolecules in HAp calcifications, such as lipids, proteins, cytochrome C and polysaccharides. The demonstration of natural DNA biomineralization, particularly in the tumor microenvironment, represents an unprecedented advance in the field, as it can pave the way to understanding the role of HAp in malignant tissues.https://www.mdpi.com/2072-6694/13/11/2658microcalcificationsDNA mineralizationbreast cancertumor microenvironmentRaman spectroscopy |
| spellingShingle | Monica Marro Anna M. Rodríguez-Rivero Cuauhtémoc Araujo-Andrade Maria Teresa Fernández-Figueras Laia Pérez-Roca Eva Castellà Jordi Navinés Antonio Mariscal Joan Francesc Julián Pau Turon Pablo Loza-Alvarez Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging Cancers microcalcifications DNA mineralization breast cancer tumor microenvironment Raman spectroscopy |
| title | Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging |
| title_full | Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging |
| title_fullStr | Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging |
| title_full_unstemmed | Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging |
| title_short | Unravelling the Encapsulation of DNA and Other Biomolecules in HAp Microcalcifications of Human Breast Cancer Tissues by Raman Imaging |
| title_sort | unravelling the encapsulation of dna and other biomolecules in hap microcalcifications of human breast cancer tissues by raman imaging |
| topic | microcalcifications DNA mineralization breast cancer tumor microenvironment Raman spectroscopy |
| url | https://www.mdpi.com/2072-6694/13/11/2658 |
| work_keys_str_mv | AT monicamarro unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT annamrodriguezrivero unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT cuauhtemocaraujoandrade unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT mariateresafernandezfigueras unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT laiaperezroca unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT evacastella unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT jordinavines unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT antoniomariscal unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT joanfrancescjulian unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT pauturon unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging AT pablolozaalvarez unravellingtheencapsulationofdnaandotherbiomoleculesinhapmicrocalcificationsofhumanbreastcancertissuesbyramanimaging |