Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics Patients
Asthma as a chronic inflammatory airway disease is considered to be the most common chronic disease that is involving genetic and environmental factors. Toll like receptors (TLRs) and other inflammatory mediators are important in modulation of inflammation. In this study, we evaluated the role of TL...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-02-01
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| Series: | Iranian Journal of Allergy, Asthma and Immunology |
| Subjects: | |
| Online Access: | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/413 |
| _version_ | 1828464198539018240 |
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| author | Hamid Bahrami Saeed Daneshmandi Hasan Heidarnazhad Ali Akbar Pourfathollah |
| author_facet | Hamid Bahrami Saeed Daneshmandi Hasan Heidarnazhad Ali Akbar Pourfathollah |
| author_sort | Hamid Bahrami |
| collection | DOAJ |
| description | Asthma as a chronic inflammatory airway disease is considered to be the most common chronic disease that is involving genetic and environmental factors. Toll like receptors (TLRs) and other inflammatory mediators are important in modulation of inflammation. In this study, we evaluated the role of TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms in the asthma susceptibility, progress, control levels and lung functions in Iranian patients. On 99 asthmatic patients and 120 normal subjects, TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms were evaluated by PCR-RFLP method recruiting Msp1 and Nco1 restriction enzymes, respectively. IgE serum levels by ELISA technique were determined and asthma diagnosis, treatment and control levels were considered using standard schemes and criteria. Our results indicated that the genotype and allele frequencies of the TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms were not significantly different between control subjects and asthmatics and were not related to in asthma features such as IgE levels, asthma history and pulmonary factors. Wherease some previous studies indicated TLRs and their polymorphisms might have some role in asthma incidence and features, our data demonstrated that TLR2 Arg753Gln and TLR4 Asp299Gly gene variants were not risk factors for asthma or its features in Iranian patients. Genetic complexity, ethnicity, influence of other genes or polymorphisms may overcome these polymorphisms in our asthmatics. |
| first_indexed | 2024-12-11T03:07:41Z |
| format | Article |
| id | doaj.art-10559b1242aa47aa87046b00d991679c |
| institution | Directory Open Access Journal |
| issn | 1735-1502 1735-5249 |
| language | English |
| last_indexed | 2024-12-11T03:07:41Z |
| publishDate | 2015-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Allergy, Asthma and Immunology |
| spelling | doaj.art-10559b1242aa47aa87046b00d991679c2022-12-22T01:22:55ZengTehran University of Medical SciencesIranian Journal of Allergy, Asthma and Immunology1735-15021735-52492015-02-01141378Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics PatientsHamid Bahrami0Saeed Daneshmandi1Hasan Heidarnazhad2Ali Akbar Pourfathollah3Department of Immunology, Faculty of Medicine, Tarbiat Modares University of Medical Sciences, Tehran, Iran.Department of Immunology, Faculty of Medicine, Tarbiat Modares University of Medical Sciences, Tehran, Iran.TB and Lung Disease Research Cancer, NRITLD, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Department of Immunology, Faculty of Medicine, Tarbiat Modares University of Medical Sciences, Tehran, Iran.Asthma as a chronic inflammatory airway disease is considered to be the most common chronic disease that is involving genetic and environmental factors. Toll like receptors (TLRs) and other inflammatory mediators are important in modulation of inflammation. In this study, we evaluated the role of TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms in the asthma susceptibility, progress, control levels and lung functions in Iranian patients. On 99 asthmatic patients and 120 normal subjects, TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms were evaluated by PCR-RFLP method recruiting Msp1 and Nco1 restriction enzymes, respectively. IgE serum levels by ELISA technique were determined and asthma diagnosis, treatment and control levels were considered using standard schemes and criteria. Our results indicated that the genotype and allele frequencies of the TLR2 Arg753Gln and TLR4 Asp299Gly polymorphisms were not significantly different between control subjects and asthmatics and were not related to in asthma features such as IgE levels, asthma history and pulmonary factors. Wherease some previous studies indicated TLRs and their polymorphisms might have some role in asthma incidence and features, our data demonstrated that TLR2 Arg753Gln and TLR4 Asp299Gly gene variants were not risk factors for asthma or its features in Iranian patients. Genetic complexity, ethnicity, influence of other genes or polymorphisms may overcome these polymorphisms in our asthmatics.https://ijaai.tums.ac.ir/index.php/ijaai/article/view/413AsthmaPolymorphismRFLP-PCRTLR2TLR4 |
| spellingShingle | Hamid Bahrami Saeed Daneshmandi Hasan Heidarnazhad Ali Akbar Pourfathollah Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics Patients Iranian Journal of Allergy, Asthma and Immunology Asthma Polymorphism RFLP-PCR TLR2 TLR4 |
| title | Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics Patients |
| title_full | Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics Patients |
| title_fullStr | Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics Patients |
| title_full_unstemmed | Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics Patients |
| title_short | Lack of Association between Toll Like Receptor-2 and Toll Like Receptor-4 Gene Polymorphisms and Other Feature in Iranian Asthmatics Patients |
| title_sort | lack of association between toll like receptor 2 and toll like receptor 4 gene polymorphisms and other feature in iranian asthmatics patients |
| topic | Asthma Polymorphism RFLP-PCR TLR2 TLR4 |
| url | https://ijaai.tums.ac.ir/index.php/ijaai/article/view/413 |
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