First isolation of West Nile virus in Brazil
BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state....
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2019-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100305&lng=en&tlng=en |
| _version_ | 1827831647359205376 |
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| author | Lívia Caricio Martins Eliana Vieira Pinto da Silva Livia Medeiros Neves Casseb Sandro Patroca da Silva Ana Cecília Ribeiro Cruz Jamilla Augusta de Sousa Pantoja Daniele Barbosa de Almeida Medeiros Arnaldo Jorge Martins Filho Ermelinda do Rosário Moutinho da Cruz Marialva Tereza Ferreira de Araújo Jedson Ferreira Cardoso Marcos Antônio Correia Rodrigues da Cunha Gilton Luiz Almada Alessandro Pecego Martins Romano Maria Guadalupe Dias Pestana Santos Gilsa Aparecida Pimenta Rodrigues Jannifer Oliveira Chiang Juarez Antonio Simões Quaresma Valéria Lima Carvalho Pedro Fernando da Costa Vasconcelos |
| author_facet | Lívia Caricio Martins Eliana Vieira Pinto da Silva Livia Medeiros Neves Casseb Sandro Patroca da Silva Ana Cecília Ribeiro Cruz Jamilla Augusta de Sousa Pantoja Daniele Barbosa de Almeida Medeiros Arnaldo Jorge Martins Filho Ermelinda do Rosário Moutinho da Cruz Marialva Tereza Ferreira de Araújo Jedson Ferreira Cardoso Marcos Antônio Correia Rodrigues da Cunha Gilton Luiz Almada Alessandro Pecego Martins Romano Maria Guadalupe Dias Pestana Santos Gilsa Aparecida Pimenta Rodrigues Jannifer Oliveira Chiang Juarez Antonio Simões Quaresma Valéria Lima Carvalho Pedro Fernando da Costa Vasconcelos |
| author_sort | Lívia Caricio Martins |
| collection | DOAJ |
| description | BACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000’s decade. |
| first_indexed | 2024-03-12T04:58:23Z |
| format | Article |
| id | doaj.art-1055bf8128c0410f98f10581e590fbc5 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:58:23Z |
| publishDate | 2019-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1055bf8128c0410f98f10581e590fbc52023-09-03T09:14:33ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602019-01-01114010.1590/0074-02760180332S0074-02762019000100305First isolation of West Nile virus in BrazilLívia Caricio MartinsEliana Vieira Pinto da SilvaLivia Medeiros Neves CassebSandro Patroca da SilvaAna Cecília Ribeiro CruzJamilla Augusta de Sousa PantojaDaniele Barbosa de Almeida MedeirosArnaldo Jorge Martins FilhoErmelinda do Rosário Moutinho da CruzMarialva Tereza Ferreira de AraújoJedson Ferreira CardosoMarcos Antônio Correia Rodrigues da CunhaGilton Luiz AlmadaAlessandro Pecego Martins RomanoMaria Guadalupe Dias Pestana SantosGilsa Aparecida Pimenta RodriguesJannifer Oliveira ChiangJuarez Antonio Simões QuaresmaValéria Lima CarvalhoPedro Fernando da Costa VasconcelosBACKGROUND Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000’s decade.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100305&lng=en&tlng=enWest Nile virushorsesBrazil |
| spellingShingle | Lívia Caricio Martins Eliana Vieira Pinto da Silva Livia Medeiros Neves Casseb Sandro Patroca da Silva Ana Cecília Ribeiro Cruz Jamilla Augusta de Sousa Pantoja Daniele Barbosa de Almeida Medeiros Arnaldo Jorge Martins Filho Ermelinda do Rosário Moutinho da Cruz Marialva Tereza Ferreira de Araújo Jedson Ferreira Cardoso Marcos Antônio Correia Rodrigues da Cunha Gilton Luiz Almada Alessandro Pecego Martins Romano Maria Guadalupe Dias Pestana Santos Gilsa Aparecida Pimenta Rodrigues Jannifer Oliveira Chiang Juarez Antonio Simões Quaresma Valéria Lima Carvalho Pedro Fernando da Costa Vasconcelos First isolation of West Nile virus in Brazil Memorias do Instituto Oswaldo Cruz West Nile virus horses Brazil |
| title | First isolation of West Nile virus in Brazil |
| title_full | First isolation of West Nile virus in Brazil |
| title_fullStr | First isolation of West Nile virus in Brazil |
| title_full_unstemmed | First isolation of West Nile virus in Brazil |
| title_short | First isolation of West Nile virus in Brazil |
| title_sort | first isolation of west nile virus in brazil |
| topic | West Nile virus horses Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762019000100305&lng=en&tlng=en |
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