DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis
Spinal facet joint osteoarthritis (FJOA) is an OA disease with pathogenesis and progression uncovered. Our present study was performed to elucidate the role of DNM3OS on spinal FJOA. In this study, spine facet joint tissue of patients were collected. In vitro and in vivo models were constructed with...
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KeAi Communications Co., Ltd.
2024-06-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054024000088 |
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| author | Jing Wang Zhenyu Yang Xiuming He Yeyang Wang Dixin Luo Wangyang Xu Hongtao Zhang Xiaozhong Zhou |
| author_facet | Jing Wang Zhenyu Yang Xiuming He Yeyang Wang Dixin Luo Wangyang Xu Hongtao Zhang Xiaozhong Zhou |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Spinal facet joint osteoarthritis (FJOA) is an OA disease with pathogenesis and progression uncovered. Our present study was performed to elucidate the role of DNM3OS on spinal FJOA. In this study, spine facet joint tissue of patients were collected. In vitro and in vivo models were constructed with SW1353 cells and rats. Hematoxylin and eosin (HE) staining, Safranin O-fast Green, Alcian blue staining, and Tolueine blue O (TBO) staining were employed for histology analyses. Quantitative PCR, western blotting, and Immunofluorescence were performed to evaluate the expression of genes. The levels of inflammatory cytokines were measured by enzyme-linked immunosorbent assay analysis. Cell Counting Kit-8 and flow cytometry were used for cell activity and apoptosis evaluation. The targeting sites between microRNA (miR)-127-5p and cadherin 11 (CDH11) were predicted TargetScan and miRbase database and confirmed by Dual-luciferase reporter assays. CHIP and EMS assay were employed to confirm the binding of LEF1and DNM3OS promoter. Our results showed that DNM3OS was found to upregulated, while miR-127-5p was downregulated in severe FJOA patients and inflammation-induced chondrosarcoma SW1353 cells. DNM3OS reduced cell activity, induced cell apoptosis and extracellular matrix (ECM) degradation by sponging miR-127-5p in vitro. miR-127-5p targeted CDH11 and inhibited wnt3a/β-catenin pathway to regulate OA in vitro. LEF1 promoted DNM3OS transcription to form a positively feedback in activated wnt3a/β-catenin pathway. In vivo rat model also confirmed that DNM3OS aggravated FJOA. In summary, DNM3OS/miR-127-5p/CDH11 enhanced Wnt3a/β-Catenin/LEF-1 pathway to form a positive feedback and aggravate spinal FJOA. |
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| institution | Directory Open Access Journal |
| issn | 2468-0540 |
| language | English |
| last_indexed | 2024-04-24T16:25:22Z |
| publishDate | 2024-06-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Non-coding RNA Research |
| spelling | doaj.art-105727b13bb94be080886cc87d11187a2024-03-31T04:37:46ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402024-06-0192294306DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritisJing Wang0Zhenyu Yang1Xiuming He2Yeyang Wang3Dixin Luo4Wangyang Xu5Hongtao Zhang6Xiaozhong Zhou7Department of Orthopaedics, Zhongshan Torch Development Zone People's Hospital, Zhongshan, 528436, ChinaSouthern Medical University, Guangzhou, 510220, ChinaDepartment of Orthopaedics, Zhongshan Torch Development Zone People's Hospital, Zhongshan, 528436, ChinaThe Spine Department, Orthopaedic Center, Guangdong Second Provincial General Hospital, Guangzhou, 510220, ChinaThe Spine Department, Orthopaedic Center, Guangdong Second Provincial General Hospital, Guangzhou, 510220, ChinaThe Spine Department, Orthopaedic Center, Guangdong Second Provincial General Hospital, Guangzhou, 510220, ChinaDepartment of Orthopaedics, Zhongshan Torch Development Zone People's Hospital, Zhongshan, 528436, ChinaSouthern Medical University, Guangzhou, 510220, China; The Spine Department, Orthopaedic Center, Guangdong Second Provincial General Hospital, Guangzhou, 510220, China; Department of Orthopedics, Hui Lai County People's Hospital of Guangdong Second Provincial General Hospital, Hui Lai, 515299, China; Corresponding author. Xingang Mid Road No.466, Haizhu District, The Spine Department, Orthopaedic Center, Guangdong Second Provincial General Hospital, Guangzhou, 510220, China.Spinal facet joint osteoarthritis (FJOA) is an OA disease with pathogenesis and progression uncovered. Our present study was performed to elucidate the role of DNM3OS on spinal FJOA. In this study, spine facet joint tissue of patients were collected. In vitro and in vivo models were constructed with SW1353 cells and rats. Hematoxylin and eosin (HE) staining, Safranin O-fast Green, Alcian blue staining, and Tolueine blue O (TBO) staining were employed for histology analyses. Quantitative PCR, western blotting, and Immunofluorescence were performed to evaluate the expression of genes. The levels of inflammatory cytokines were measured by enzyme-linked immunosorbent assay analysis. Cell Counting Kit-8 and flow cytometry were used for cell activity and apoptosis evaluation. The targeting sites between microRNA (miR)-127-5p and cadherin 11 (CDH11) were predicted TargetScan and miRbase database and confirmed by Dual-luciferase reporter assays. CHIP and EMS assay were employed to confirm the binding of LEF1and DNM3OS promoter. Our results showed that DNM3OS was found to upregulated, while miR-127-5p was downregulated in severe FJOA patients and inflammation-induced chondrosarcoma SW1353 cells. DNM3OS reduced cell activity, induced cell apoptosis and extracellular matrix (ECM) degradation by sponging miR-127-5p in vitro. miR-127-5p targeted CDH11 and inhibited wnt3a/β-catenin pathway to regulate OA in vitro. LEF1 promoted DNM3OS transcription to form a positively feedback in activated wnt3a/β-catenin pathway. In vivo rat model also confirmed that DNM3OS aggravated FJOA. In summary, DNM3OS/miR-127-5p/CDH11 enhanced Wnt3a/β-Catenin/LEF-1 pathway to form a positive feedback and aggravate spinal FJOA.http://www.sciencedirect.com/science/article/pii/S2468054024000088ceRNA networkSpinal facet joint osteoarthritisDNM3OSmiR-127-5pWnt/β-Catenin/LEF-1 |
| spellingShingle | Jing Wang Zhenyu Yang Xiuming He Yeyang Wang Dixin Luo Wangyang Xu Hongtao Zhang Xiaozhong Zhou DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis Non-coding RNA Research ceRNA network Spinal facet joint osteoarthritis DNM3OS miR-127-5p Wnt/β-Catenin/LEF-1 |
| title | DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis |
| title_full | DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis |
| title_fullStr | DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis |
| title_full_unstemmed | DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis |
| title_short | DNM3OS/miR-127-5p/CDH11, activates Wnt3a/β-catenin/LEF-1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis |
| title_sort | dnm3os mir 127 5p cdh11 activates wnt3a β catenin lef 1 pathway to form a positive feedback and aggravate spine facet joint osteoarthritis |
| topic | ceRNA network Spinal facet joint osteoarthritis DNM3OS miR-127-5p Wnt/β-Catenin/LEF-1 |
| url | http://www.sciencedirect.com/science/article/pii/S2468054024000088 |
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