Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR

Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens.To characterizes P. aeruginosa strains that are widespread in patients in Iraq, 90 clinical samples were collected from wounds, burn, ear infection and urinary tract in...

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Main Authors: Marrib N. Rasheed, Noor S. Latteef, Ashwak B. Jassim
Format: Article
Language:English
Published: Faculty of Veterinary Medicine, University of Kufa 2016-10-01
Series:Kufa Journal for Veterinary Medical Sciences
Subjects:
Online Access:https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4275
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author Marrib N. Rasheed
Noor S. Latteef
Ashwak B. Jassim
author_facet Marrib N. Rasheed
Noor S. Latteef
Ashwak B. Jassim
author_sort Marrib N. Rasheed
collection DOAJ
description Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens.To characterizes P. aeruginosa strains that are widespread in patients in Iraq, 90 clinical samples were collected from wounds, burn, ear infection and urinary tract infection taken from three general hospitals of different areas of the region in Baghdad .  Methods for isolation and identifying P. aeruginosa based upon culture methods coupled with biochemical tests, were used in this study. The results show that, the selective medium (cetrimide agar) at 42˚C aerobically had highest recovery in the isolation of P. aeruginosa strains, they were produced greenish-yellow or blue pigment colonies, catalase and oxidase was positive whereas negative for methyl red, and indole.; however, some of these methods are time consuming and may not be very accurate whereas API 20E is rapid method which performs at least 20 different biochemical tests at once, however it proved difficult to obtain additional information concerning the relationship between these strains. Molecular study for identifying  p.aeruginosa include  DNA extraction  , than Real time assay by using  powerchek  P. aeruginosa  Real time PCR Kit with probe, the results showed that RT-PCR has found to be rapid and more sensitive and specific in identification of P. aeruginosa, however Real time PCR Kit with probe, specific marker is recommended
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spelling doaj.art-105758ffba634dbebf335691f534e4e42024-01-06T10:17:28ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782016-10-0171B10.36326/kjvs/2016/v7i1B4275Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCRMarrib N. RasheedNoor S. LatteefAshwak B. Jassim Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens.To characterizes P. aeruginosa strains that are widespread in patients in Iraq, 90 clinical samples were collected from wounds, burn, ear infection and urinary tract infection taken from three general hospitals of different areas of the region in Baghdad .  Methods for isolation and identifying P. aeruginosa based upon culture methods coupled with biochemical tests, were used in this study. The results show that, the selective medium (cetrimide agar) at 42˚C aerobically had highest recovery in the isolation of P. aeruginosa strains, they were produced greenish-yellow or blue pigment colonies, catalase and oxidase was positive whereas negative for methyl red, and indole.; however, some of these methods are time consuming and may not be very accurate whereas API 20E is rapid method which performs at least 20 different biochemical tests at once, however it proved difficult to obtain additional information concerning the relationship between these strains. Molecular study for identifying  p.aeruginosa include  DNA extraction  , than Real time assay by using  powerchek  P. aeruginosa  Real time PCR Kit with probe, the results showed that RT-PCR has found to be rapid and more sensitive and specific in identification of P. aeruginosa, however Real time PCR Kit with probe, specific marker is recommended https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4275Opal genepseudomonas aeruginosaRT-PCR
spellingShingle Marrib N. Rasheed
Noor S. Latteef
Ashwak B. Jassim
Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR
Kufa Journal for Veterinary Medical Sciences
Opal gene
pseudomonas aeruginosa
RT-PCR
title Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR
title_full Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR
title_fullStr Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR
title_full_unstemmed Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR
title_short Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR
title_sort rapid identification of pseudomonas aeruginosa by using real time pcr
topic Opal gene
pseudomonas aeruginosa
RT-PCR
url https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4275
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