<i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i>
Sugar transport from the source leaf to the sink organ is critical for seed development and crop yield, as well as for responding to abiotic stress. SWEETs (sugar will eventually be exported transporters) mediate sugar efflux into the reproductive sink and are therefore considered key candidate prot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/15/2818 |
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| author | Zhihua Ye Bingshuai Du Jing Zhou Yibo Cao Lingyun Zhang |
| author_facet | Zhihua Ye Bingshuai Du Jing Zhou Yibo Cao Lingyun Zhang |
| author_sort | Zhihua Ye |
| collection | DOAJ |
| description | Sugar transport from the source leaf to the sink organ is critical for seed development and crop yield, as well as for responding to abiotic stress. SWEETs (sugar will eventually be exported transporters) mediate sugar efflux into the reproductive sink and are therefore considered key candidate proteins for sugar unloading during seed development. However, the specific mechanism underlying the sugar unloading to seeds in <i>Camellia oleifera</i> remains elusive. Here, we identified a SWEET gene named CoSWEET10, which belongs to Clade III and has high expression levels in the seeds of <i>C. oleifera</i>. CoSWEET10 is a plasma membrane-localized protein. The complementation assay of CoSWEET10 in SUSY7/ura3 and EBY.VW4000 yeast strains showed that CoSWEET10 has the ability to transport sucrose, glucose, and fructose. Through the <i>C. oleifera</i> seeds in vitro culture, we found that the expression of CoSWEET10 can be induced by hexose and sucrose, and especially glucose. By generating the restoration lines of CoSWEET10 in <i>Arabidopsis atsweet10</i>, we found that CoSWEET10 restored the seed defect phenotype of the mutant by regulating soluble sugar accumulation and increased plant drought tolerance. Collectively, our study demonstrates that CoSWEET10 plays a dual role in promoting seed development and enhancing plant drought resistance as a sucrose and hexose transporter. |
| first_indexed | 2024-03-11T00:18:05Z |
| format | Article |
| id | doaj.art-105d9304304f4ce39bc0cb72d75a4b77 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T00:18:05Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-105d9304304f4ce39bc0cb72d75a4b772023-11-18T23:26:16ZengMDPI AGPlants2223-77472023-07-011215281810.3390/plants12152818<i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i>Zhihua Ye0Bingshuai Du1Jing Zhou2Yibo Cao3Lingyun Zhang4Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing 100083, ChinaKey Laboratory of Forest Silviculture and Conservation of the Ministry of Education, The College of Forestry, Beijing Forestry University, Beijing 100083, ChinaSugar transport from the source leaf to the sink organ is critical for seed development and crop yield, as well as for responding to abiotic stress. SWEETs (sugar will eventually be exported transporters) mediate sugar efflux into the reproductive sink and are therefore considered key candidate proteins for sugar unloading during seed development. However, the specific mechanism underlying the sugar unloading to seeds in <i>Camellia oleifera</i> remains elusive. Here, we identified a SWEET gene named CoSWEET10, which belongs to Clade III and has high expression levels in the seeds of <i>C. oleifera</i>. CoSWEET10 is a plasma membrane-localized protein. The complementation assay of CoSWEET10 in SUSY7/ura3 and EBY.VW4000 yeast strains showed that CoSWEET10 has the ability to transport sucrose, glucose, and fructose. Through the <i>C. oleifera</i> seeds in vitro culture, we found that the expression of CoSWEET10 can be induced by hexose and sucrose, and especially glucose. By generating the restoration lines of CoSWEET10 in <i>Arabidopsis atsweet10</i>, we found that CoSWEET10 restored the seed defect phenotype of the mutant by regulating soluble sugar accumulation and increased plant drought tolerance. Collectively, our study demonstrates that CoSWEET10 plays a dual role in promoting seed development and enhancing plant drought resistance as a sucrose and hexose transporter.https://www.mdpi.com/2223-7747/12/15/2818<i>Camellia oleifera</i>sugar transportSWEETsseed developmentdrought tolerance |
| spellingShingle | Zhihua Ye Bingshuai Du Jing Zhou Yibo Cao Lingyun Zhang <i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i> Plants <i>Camellia oleifera</i> sugar transport SWEETs seed development drought tolerance |
| title | <i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i> |
| title_full | <i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i> |
| title_fullStr | <i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | <i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i> |
| title_short | <i>Camellia oleifera</i> CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic <i>Arabidopsis</i> |
| title_sort | i camellia oleifera i cosweet10 is crucial for seed development and drought resistance by mediating sugar transport in transgenic i arabidopsis i |
| topic | <i>Camellia oleifera</i> sugar transport SWEETs seed development drought tolerance |
| url | https://www.mdpi.com/2223-7747/12/15/2818 |
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