Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption
Electrospun polymer membranes are regarded as prospective biosensor components due to their large specific surface area and diverse opportunities for chemical modifications. However, their intricate porous structure can impede diffusion and render some analyte-binding sites inaccessible. To overcome...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Micro |
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| Online Access: | https://www.mdpi.com/2673-8023/3/2/38 |
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| author | Aitsana Maslakova Kirill Prusakov Anastasia Sidorova Elizaveta Pavlova Alla Ramonova Dmitry Bagrov |
| author_facet | Aitsana Maslakova Kirill Prusakov Anastasia Sidorova Elizaveta Pavlova Alla Ramonova Dmitry Bagrov |
| author_sort | Aitsana Maslakova |
| collection | DOAJ |
| description | Electrospun polymer membranes are regarded as prospective biosensor components due to their large specific surface area and diverse opportunities for chemical modifications. However, their intricate porous structure can impede diffusion and render some analyte-binding sites inaccessible. To overcome these diffusion limitations and improve analyte adsorption onto the polymer, a pressure-driven sample flow through the membrane can be employed. To date, the efficiency of pressure-driven analyte delivery into these membranes has not been quantified. Here, we compare forced flow and passive sample diffusion through poly(dioxanone) electrospun membranes. We examine two model analytes, BSA and interleukin-1 beta (IL1b), to address both non-specific and specific binding. Following exposure of the membranes to the test solutions, we measured the residual concentrations of the analytes using fluorometry and enzyme-linked immunosorbent assay (ELISA) techniques. The pressure-driven sample loading was superior to passive diffusion, with a 2.8–11.5-fold change for physical adsorption and a 2.4–3.4-fold difference for specific binding. Our data can be useful for the development of immunoassays and microfluidic devices. |
| first_indexed | 2024-03-11T02:08:55Z |
| format | Article |
| id | doaj.art-1062581ea9a646e886ed7d3834c0739d |
| institution | Directory Open Access Journal |
| issn | 2673-8023 |
| language | English |
| last_indexed | 2024-03-11T02:08:55Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micro |
| spelling | doaj.art-1062581ea9a646e886ed7d3834c0739d2023-11-18T11:37:56ZengMDPI AGMicro2673-80232023-05-013256657710.3390/micro3020038Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte AdsorptionAitsana Maslakova0Kirill Prusakov1Anastasia Sidorova2Elizaveta Pavlova3Alla Ramonova4Dmitry Bagrov5Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaLopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaFaculty of Biology, Lomonosov Moscow State University, 119234 Moscow, RussiaElectrospun polymer membranes are regarded as prospective biosensor components due to their large specific surface area and diverse opportunities for chemical modifications. However, their intricate porous structure can impede diffusion and render some analyte-binding sites inaccessible. To overcome these diffusion limitations and improve analyte adsorption onto the polymer, a pressure-driven sample flow through the membrane can be employed. To date, the efficiency of pressure-driven analyte delivery into these membranes has not been quantified. Here, we compare forced flow and passive sample diffusion through poly(dioxanone) electrospun membranes. We examine two model analytes, BSA and interleukin-1 beta (IL1b), to address both non-specific and specific binding. Following exposure of the membranes to the test solutions, we measured the residual concentrations of the analytes using fluorometry and enzyme-linked immunosorbent assay (ELISA) techniques. The pressure-driven sample loading was superior to passive diffusion, with a 2.8–11.5-fold change for physical adsorption and a 2.4–3.4-fold difference for specific binding. Our data can be useful for the development of immunoassays and microfluidic devices.https://www.mdpi.com/2673-8023/3/2/38electrospun membraneadsorptionbiosensoranalytepressure-driven sample flow |
| spellingShingle | Aitsana Maslakova Kirill Prusakov Anastasia Sidorova Elizaveta Pavlova Alla Ramonova Dmitry Bagrov Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption Micro electrospun membrane adsorption biosensor analyte pressure-driven sample flow |
| title | Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption |
| title_full | Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption |
| title_fullStr | Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption |
| title_full_unstemmed | Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption |
| title_short | Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption |
| title_sort | pressure driven sample flow through an electrospun membrane increases the analyte adsorption |
| topic | electrospun membrane adsorption biosensor analyte pressure-driven sample flow |
| url | https://www.mdpi.com/2673-8023/3/2/38 |
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