Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications
Nanodiamonds have been studied for several biomedical applications due to their inherent biocompatibility and low cytotoxicity. Recent investigations have shown perspectives in using fluorescent nanodiamonds as nanothermometers because of their optical properties’ dependence on temperature. Easy and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/9/4065 |
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| author | Francisco Pedroza-Montero Karla Santacruz-Gómez Mónica Acosta-Elías Erika Silva-Campa Diana Meza-Figueroa Diego Soto-Puebla Beatriz Castaneda Efraín Urrutia-Bañuelos Osiris Álvarez-Bajo Sofía Navarro-Espinoza Raúl Riera Martín Pedroza-Montero |
| author_facet | Francisco Pedroza-Montero Karla Santacruz-Gómez Mónica Acosta-Elías Erika Silva-Campa Diana Meza-Figueroa Diego Soto-Puebla Beatriz Castaneda Efraín Urrutia-Bañuelos Osiris Álvarez-Bajo Sofía Navarro-Espinoza Raúl Riera Martín Pedroza-Montero |
| author_sort | Francisco Pedroza-Montero |
| collection | DOAJ |
| description | Nanodiamonds have been studied for several biomedical applications due to their inherent biocompatibility and low cytotoxicity. Recent investigations have shown perspectives in using fluorescent nanodiamonds as nanothermometers because of their optical properties’ dependence on temperature. Easy and accurate localized temperature sensing is essential in a wide variety of scientific fields. Our work demonstrated how the fluorescence spectrum of high-pressure high-temperature fluorescent nanodiamonds of three different sizes: 35 nm, 70 nm and 100 nm, changes with temperature within an important biological temperature range (25 °C to 60 °C). Taking advantage of this phenomenon, we obtained nanothermic scales (NS) from the zero phonon lines (ZPL) of the NV<sup>0</sup> and NV<sup>−</sup> colour centres. In particular, the 100 nm-sized features the more intense fluorescence spectra whose linear dependence with temperature achieved 0.98 R<sup>2</sup> data representation values for both NV<sup>0</sup> and NV<sup>−</sup>. This model predicts temperature for all used nanodiamonds with sensitivities ranging from 5.73% °C<sup>−1</sup> to 6.994% °C<sup>−1</sup> (NV<sup>0</sup>) and from 4.14% °C<sup>−1</sup> to 6.475% °C<sup>−1</sup> (NV<sup>−</sup>). Furthermore, the non-cytotoxic interaction with HeLa cells tested in our study enables the potential use of fluorescence nanodiamonds to measure temperatures in similar nano and microcellular aqueous environments with a simple spectroscopic setup. |
| first_indexed | 2024-03-10T11:49:48Z |
| format | Article |
| id | doaj.art-10680899ddca4623947440b8615d21c0 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T11:49:48Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-10680899ddca4623947440b8615d21c02023-11-21T17:47:20ZengMDPI AGApplied Sciences2076-34172021-04-01119406510.3390/app11094065Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical ApplicationsFrancisco Pedroza-Montero0Karla Santacruz-Gómez1Mónica Acosta-Elías2Erika Silva-Campa3Diana Meza-Figueroa4Diego Soto-Puebla5Beatriz Castaneda6Efraín Urrutia-Bañuelos7Osiris Álvarez-Bajo8Sofía Navarro-Espinoza9Raúl Riera10Martín Pedroza-Montero11Physics PhD Program, Department of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics, University of Sonora, Rosales y Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Geology, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics, University of Sonora, Rosales y Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoNanotechnology PhD Program, Department of Physics, University of Sonora, Rosales y Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoDepartment of Physics Research, University of Sonora, Rosales and Encinas, Hermosillo 83000, Sonora, MexicoNanodiamonds have been studied for several biomedical applications due to their inherent biocompatibility and low cytotoxicity. Recent investigations have shown perspectives in using fluorescent nanodiamonds as nanothermometers because of their optical properties’ dependence on temperature. Easy and accurate localized temperature sensing is essential in a wide variety of scientific fields. Our work demonstrated how the fluorescence spectrum of high-pressure high-temperature fluorescent nanodiamonds of three different sizes: 35 nm, 70 nm and 100 nm, changes with temperature within an important biological temperature range (25 °C to 60 °C). Taking advantage of this phenomenon, we obtained nanothermic scales (NS) from the zero phonon lines (ZPL) of the NV<sup>0</sup> and NV<sup>−</sup> colour centres. In particular, the 100 nm-sized features the more intense fluorescence spectra whose linear dependence with temperature achieved 0.98 R<sup>2</sup> data representation values for both NV<sup>0</sup> and NV<sup>−</sup>. This model predicts temperature for all used nanodiamonds with sensitivities ranging from 5.73% °C<sup>−1</sup> to 6.994% °C<sup>−1</sup> (NV<sup>0</sup>) and from 4.14% °C<sup>−1</sup> to 6.475% °C<sup>−1</sup> (NV<sup>−</sup>). Furthermore, the non-cytotoxic interaction with HeLa cells tested in our study enables the potential use of fluorescence nanodiamonds to measure temperatures in similar nano and microcellular aqueous environments with a simple spectroscopic setup.https://www.mdpi.com/2076-3417/11/9/4065nanodiamondnanothermometerNV centresfluorescencebioimagingHeLa |
| spellingShingle | Francisco Pedroza-Montero Karla Santacruz-Gómez Mónica Acosta-Elías Erika Silva-Campa Diana Meza-Figueroa Diego Soto-Puebla Beatriz Castaneda Efraín Urrutia-Bañuelos Osiris Álvarez-Bajo Sofía Navarro-Espinoza Raúl Riera Martín Pedroza-Montero Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications Applied Sciences nanodiamond nanothermometer NV centres fluorescence bioimaging HeLa |
| title | Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications |
| title_full | Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications |
| title_fullStr | Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications |
| title_full_unstemmed | Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications |
| title_short | Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications |
| title_sort | thermometric characterization of fluorescent nanodiamonds suitable for biomedical applications |
| topic | nanodiamond nanothermometer NV centres fluorescence bioimaging HeLa |
| url | https://www.mdpi.com/2076-3417/11/9/4065 |
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