Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts
AIM: To investigate the anti-inflammatory effects of the sesquiterpenes α-humulene and β-caryophyllene on pterygium fibroblasts. METHODS: Primary cultures of pterygium fibroblasts were established. Third passage pterygium fibroblasts were exposed to α-humulene and β-caryophyllene separately and toge...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2022-12-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/en_publish/2022/12/20221202.pdf |
| _version_ | 1811179362970501120 |
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| author | Magda Massae Hata Viveiros Márcia Guimarães Silva José Galberto Martins da Costa Anselmo Gomes de Oliveira Carolina Rubio Carlos Roberto Padovani Cláudia Aparecida Rainho Silvana Artioli Schellini |
| author_facet | Magda Massae Hata Viveiros Márcia Guimarães Silva José Galberto Martins da Costa Anselmo Gomes de Oliveira Carolina Rubio Carlos Roberto Padovani Cláudia Aparecida Rainho Silvana Artioli Schellini |
| author_sort | Magda Massae Hata Viveiros |
| collection | DOAJ |
| description | AIM: To investigate the anti-inflammatory effects of the sesquiterpenes α-humulene and β-caryophyllene on pterygium fibroblasts. METHODS: Primary cultures of pterygium fibroblasts were established. Third passage pterygium fibroblasts were exposed to α-humulene and β-caryophyllene separately and together. The cell viability was assessed by 3- (4,5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide (MTT) assay at 12, 24, 48, and 72h after exposure. The levels of the inflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α and IL-10 in the conditioned culture medium were assessed by enzyme-linked immunosorbent assay (ELISA) at 12, 24 and 48h after exposure. Data were statistically analyzed using Friedman repeated measures analysis of variances on ranks. RESULTS: The 25 μmol/L β-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure (P=0.041). The levels of IL-1β, IL-8, IL-10, and TNF-α were very low and had no statistically significant variations after exposure to α-humulene, β-caryophyllene, or both compounds together. CONCLUSION: The exposure to 25 μmol/L of β-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after 48h. This sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium. |
| first_indexed | 2024-04-11T06:33:26Z |
| format | Article |
| id | doaj.art-106919b8777e4c628abd8204c9b06795 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-11T06:33:26Z |
| publishDate | 2022-12-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-106919b8777e4c628abd8204c9b067952022-12-22T04:39:53ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982022-12-0115121903190710.18240/ijo.2022.12.0220221202Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblastsMagda Massae Hata Viveiros0Márcia Guimarães Silva1José Galberto Martins da Costa2Anselmo Gomes de Oliveira3Carolina Rubio4Carlos Roberto Padovani5Cláudia Aparecida Rainho6Silvana Artioli Schellini7Silvana Artioli Schellini. Department of Ophthalmology of Botucatu Medical School-Campus UNESP de Botucatu, Botucatu, Sao Paulo 01049-010, Brazil. sschellini@gmail.comDepartment of Pathology, School of Medicine of Botucatu, São Paulo State University–UNESP, Botucatu, Sao Paulo 01049-010, BrazilDepartment of Biological Chemistry, Regional University of Cariri, Crato, Ceará 66100-900, BrazilDepartment of Pharmacology, School of Pharmaceutical Sciences, São Paulo State University–UNESP, Araraquara, Sao Paulo 01049-010, BrazilBiological Sciences, Institute of Biosciences of Botucatu, São Paulo State University–UNESP, Botucatu, Sao Paulo 01049-010, BrazilDepartment of Biostatistics, São Paulo State University–UNESP, Botucatu, Sao Paulo 01049-010, BrazilDepartment of Biostatistics, São Paulo State University–UNESP, Botucatu, Sao Paulo 01049-010, BrazilDepartment of Ophthalmology, School of Medicine of Botucatu, São Paulo State University–UNESP, Botucatu, Sao Paulo 18618-000, BrazilAIM: To investigate the anti-inflammatory effects of the sesquiterpenes α-humulene and β-caryophyllene on pterygium fibroblasts. METHODS: Primary cultures of pterygium fibroblasts were established. Third passage pterygium fibroblasts were exposed to α-humulene and β-caryophyllene separately and together. The cell viability was assessed by 3- (4,5-dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide (MTT) assay at 12, 24, 48, and 72h after exposure. The levels of the inflammatory cytokines interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α and IL-10 in the conditioned culture medium were assessed by enzyme-linked immunosorbent assay (ELISA) at 12, 24 and 48h after exposure. Data were statistically analyzed using Friedman repeated measures analysis of variances on ranks. RESULTS: The 25 μmol/L β-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure (P=0.041). The levels of IL-1β, IL-8, IL-10, and TNF-α were very low and had no statistically significant variations after exposure to α-humulene, β-caryophyllene, or both compounds together. CONCLUSION: The exposure to 25 μmol/L of β-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after 48h. This sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.http://ies.ijo.cn/en_publish/2022/12/20221202.pdfpterygiumfibroblastsβ-caryophylleneα-humulenecytokines |
| spellingShingle | Magda Massae Hata Viveiros Márcia Guimarães Silva José Galberto Martins da Costa Anselmo Gomes de Oliveira Carolina Rubio Carlos Roberto Padovani Cláudia Aparecida Rainho Silvana Artioli Schellini Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts International Journal of Ophthalmology pterygium fibroblasts β-caryophyllene α-humulene cytokines |
| title | Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts |
| title_full | Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts |
| title_fullStr | Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts |
| title_full_unstemmed | Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts |
| title_short | Anti-inflammatory effects of α-humulene and β-caryophyllene on pterygium fibroblasts |
| title_sort | anti inflammatory effects of α humulene and β caryophyllene on pterygium fibroblasts |
| topic | pterygium fibroblasts β-caryophyllene α-humulene cytokines |
| url | http://ies.ijo.cn/en_publish/2022/12/20221202.pdf |
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