Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021)
Objective:This study aimed to evaluate the polymerase chain reaction (PCR) and immunofluorescence antibody test (IFAT) results of suspected samples with canine leishmaniasis (CanL) that were sent to the Parasitology Department Laboratories of the Veterinary Faculty in Aydın Adnan Menderes University...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2022-03-01
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| Series: | Türkiye Parazitoloji Dergisi |
| Subjects: | |
| Online Access: |
http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/evaluation-of-cases-with-suspected-canine-leishman/50985
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| _version_ | 1797916202867097600 |
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| author | Metin Pekağırbaş Serkan Bakırcı Hüseyin Bilgin Bilgiç Selin Hacılarlıoğlu Tülin Karagenç |
| author_facet | Metin Pekağırbaş Serkan Bakırcı Hüseyin Bilgin Bilgiç Selin Hacılarlıoğlu Tülin Karagenç |
| author_sort | Metin Pekağırbaş |
| collection | DOAJ |
| description | Objective:This study aimed to evaluate the polymerase chain reaction (PCR) and immunofluorescence antibody test (IFAT) results of suspected samples with canine leishmaniasis (CanL) that were sent to the Parasitology Department Laboratories of the Veterinary Faculty in Aydın Adnan Menderes University.Methods:The age, gender, and breed of the dogs to be evaluated for CanL were recorded, and IFAT was performed using 80 blood serum samples collected from them. Additionally, after the isolation of genomic DNA of 27 blood samples, PCR of these samples was performed using primers that amplify the 145 bp kDNA region of Leishmania species.Results:Thirty-seven (46.25%) of the serum samples were seropositive in at least one dilution (1/64 or 1/128) according to IFAT. Five (18.5%) of the twenty-seven samples were positive for Leishmania DNA according to PCR. According to IFAT, 38.7% of male dogs and 59% of female dogs were positive. The highest number of seropositive samples were detected in dogs aged 3-5 years (11/27).Conclusion:Considering the zoonotic potential of leishmaniasis, which is considered endemic in the region, and the high positivity of the IFAT/PCR results, veterinarians should use advanced diagnostic methods, especially serological and molecular tests, in dogs with suspected CanL. The data obtained show that the risk of infection caused by Leishmania spp. is high in the region. Therefore, it is important to routinely ensure the control of CanL to protect both human and animal health. |
| first_indexed | 2024-04-10T12:53:28Z |
| format | Article |
| id | doaj.art-106a35a35e76427a8c1c6b6f46a43105 |
| institution | Directory Open Access Journal |
| issn | 1300-6320 2146-3077 |
| language | English |
| last_indexed | 2024-04-10T12:53:28Z |
| publishDate | 2022-03-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türkiye Parazitoloji Dergisi |
| spelling | doaj.art-106a35a35e76427a8c1c6b6f46a431052023-02-15T16:13:36ZengGalenos YayineviTürkiye Parazitoloji Dergisi1300-63202146-30772022-03-01461283310.4274/tpd.galenos.2021.7029913049054Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021)Metin Pekağırbaş0Serkan Bakırcı1Hüseyin Bilgin Bilgiç2Selin Hacılarlıoğlu3Tülin Karagenç4 Aydın Adnan Menderes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Aydın Adnan Menderes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Türkiye Objective:This study aimed to evaluate the polymerase chain reaction (PCR) and immunofluorescence antibody test (IFAT) results of suspected samples with canine leishmaniasis (CanL) that were sent to the Parasitology Department Laboratories of the Veterinary Faculty in Aydın Adnan Menderes University.Methods:The age, gender, and breed of the dogs to be evaluated for CanL were recorded, and IFAT was performed using 80 blood serum samples collected from them. Additionally, after the isolation of genomic DNA of 27 blood samples, PCR of these samples was performed using primers that amplify the 145 bp kDNA region of Leishmania species.Results:Thirty-seven (46.25%) of the serum samples were seropositive in at least one dilution (1/64 or 1/128) according to IFAT. Five (18.5%) of the twenty-seven samples were positive for Leishmania DNA according to PCR. According to IFAT, 38.7% of male dogs and 59% of female dogs were positive. The highest number of seropositive samples were detected in dogs aged 3-5 years (11/27).Conclusion:Considering the zoonotic potential of leishmaniasis, which is considered endemic in the region, and the high positivity of the IFAT/PCR results, veterinarians should use advanced diagnostic methods, especially serological and molecular tests, in dogs with suspected CanL. The data obtained show that the risk of infection caused by Leishmania spp. is high in the region. Therefore, it is important to routinely ensure the control of CanL to protect both human and animal health. http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/evaluation-of-cases-with-suspected-canine-leishman/50985 ifatdogleishmaniapcrretrospective |
| spellingShingle | Metin Pekağırbaş Serkan Bakırcı Hüseyin Bilgin Bilgiç Selin Hacılarlıoğlu Tülin Karagenç Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021) Türkiye Parazitoloji Dergisi ifat dog leishmania pcr retrospective |
| title | Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021) |
| title_full | Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021) |
| title_fullStr | Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021) |
| title_full_unstemmed | Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021) |
| title_short | Evaluation of Cases with Suspected Canine Leishmaniasis History: A Five-year Retrospective Study (2016-2021) |
| title_sort | evaluation of cases with suspected canine leishmaniasis history a five year retrospective study 2016 2021 |
| topic | ifat dog leishmania pcr retrospective |
| url |
http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/evaluation-of-cases-with-suspected-canine-leishman/50985
|
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