Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles
Nowadays, various industries using nanomaterials are growing rapidly, and in particular, as the commercialization and use of nanomaterials increase in the cosmetic field, the possibility of exposure of nanomaterials to the skin of product producers and consumers is increasing. Due to the unique prop...
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MDPI AG
2021-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/8/2140 |
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| author | Sung-Hyun Kim Dong Han Lee SeoYoon Choi Jun-Young Yang Kikyung Jung Jayoung Jeong Jae Ho Oh Jin Hee Lee |
| author_facet | Sung-Hyun Kim Dong Han Lee SeoYoon Choi Jun-Young Yang Kikyung Jung Jayoung Jeong Jae Ho Oh Jin Hee Lee |
| author_sort | Sung-Hyun Kim |
| collection | DOAJ |
| description | Nowadays, various industries using nanomaterials are growing rapidly, and in particular, as the commercialization and use of nanomaterials increase in the cosmetic field, the possibility of exposure of nanomaterials to the skin of product producers and consumers is increasing. Due to the unique properties of nanomaterials with a very small size, they can act as hapten and induce immune responses and skin sensitization, so accurate identification of toxicity is required. Therefore, we selected silica nanomaterials used in various fields such as cosmetics and biomaterials and evaluated the skin sensitization potential step-by-step according to in-vitro and in-vivo alternative test methods. KeratinoSens<sup>TM</sup> cells of modified keratinocyte and THP-1 cells mimicking dendritic-cells were treated with silica nanoparticles, and their potential for skin sensitization and cytotoxicity were evaluated, respectively. We also confirmed the sensitizing ability of silica nanoparticles in the auricle-lymph nodes of BALB/C mice by in-vivo analysis. As a result, silica nanoparticles showed high protein binding and reactive oxygen species (ROS) mediated cytotoxicity, but no significant observation of skin sensitization indicators was observed. Although more studies are needed to elucidate the mechanism of skin sensitization by nanomaterials, the results of this study showed that silica nanoparticles did not induce skin sensitization. |
| first_indexed | 2024-03-10T08:30:52Z |
| format | Article |
| id | doaj.art-106a94a43ebe47f9af218f9c7f29c666 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T08:30:52Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-106a94a43ebe47f9af218f9c7f29c6662023-11-22T09:01:05ZengMDPI AGNanomaterials2079-49912021-08-01118214010.3390/nano11082140Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica NanoparticlesSung-Hyun Kim0Dong Han Lee1SeoYoon Choi2Jun-Young Yang3Kikyung Jung4Jayoung Jeong5Jae Ho Oh6Jin Hee Lee7Division of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaDivision of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaDivision of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaDivision of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaDivision of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaDivision of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaDivision of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaDivision of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, KoreaNowadays, various industries using nanomaterials are growing rapidly, and in particular, as the commercialization and use of nanomaterials increase in the cosmetic field, the possibility of exposure of nanomaterials to the skin of product producers and consumers is increasing. Due to the unique properties of nanomaterials with a very small size, they can act as hapten and induce immune responses and skin sensitization, so accurate identification of toxicity is required. Therefore, we selected silica nanomaterials used in various fields such as cosmetics and biomaterials and evaluated the skin sensitization potential step-by-step according to in-vitro and in-vivo alternative test methods. KeratinoSens<sup>TM</sup> cells of modified keratinocyte and THP-1 cells mimicking dendritic-cells were treated with silica nanoparticles, and their potential for skin sensitization and cytotoxicity were evaluated, respectively. We also confirmed the sensitizing ability of silica nanoparticles in the auricle-lymph nodes of BALB/C mice by in-vivo analysis. As a result, silica nanoparticles showed high protein binding and reactive oxygen species (ROS) mediated cytotoxicity, but no significant observation of skin sensitization indicators was observed. Although more studies are needed to elucidate the mechanism of skin sensitization by nanomaterials, the results of this study showed that silica nanoparticles did not induce skin sensitization.https://www.mdpi.com/2079-4991/11/8/2140silicananoparticlesskin sensitizationKeratinoSens<sup>TM</sup>h-CLATLLNA |
| spellingShingle | Sung-Hyun Kim Dong Han Lee SeoYoon Choi Jun-Young Yang Kikyung Jung Jayoung Jeong Jae Ho Oh Jin Hee Lee Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles Nanomaterials silica nanoparticles skin sensitization KeratinoSens<sup>TM</sup> h-CLAT LLNA |
| title | Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles |
| title_full | Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles |
| title_fullStr | Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles |
| title_full_unstemmed | Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles |
| title_short | Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles |
| title_sort | skin sensitization potential and cellular ros induced cytotoxicity of silica nanoparticles |
| topic | silica nanoparticles skin sensitization KeratinoSens<sup>TM</sup> h-CLAT LLNA |
| url | https://www.mdpi.com/2079-4991/11/8/2140 |
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