Lactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler
Lactobacillus fermentum 1.2029 strain with high adhesion was selected for studying the mucin expression of the small intestine epithelium of broilers after the strain was orally administrated. The strain was allowed to adhere to Caco-2 and to a hydrophobic solvent to evaluate its adhesive propertie...
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2012-10-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/49/4/49_0110146/_pdf/-char/en |
| _version_ | 1827937997851459584 |
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| author | Li Cao Xiaojun Yang Feifei Sun Chanjuan Liu Junhu Yao |
| author_facet | Li Cao Xiaojun Yang Feifei Sun Chanjuan Liu Junhu Yao |
| author_sort | Li Cao |
| collection | DOAJ |
| description | Lactobacillus fermentum 1.2029 strain with high adhesion was selected for studying the mucin expression of the small intestine epithelium of broilers after the strain was orally administrated. The strain was allowed to adhere to Caco-2 and to a hydrophobic solvent to evaluate its adhesive properties. Adhesion to intestinal mucin of chickens was also tested to further characterize the in vivo adhesion of the strain. Afterward, the acid and bile salt tolerance of the strain was also evaluated. L fermentum 1.2029 showed good adhesive ability and was able to survive in low pH conditions and in the presence of bile salts. The in vivo study showed that the strain significantly increased goblet cell density in the jejunum and the level of MUC2 mRNA in both the jejunum and ileum (P<0.05). L. fermentum 1.2029 treatment didn’t affect the thickness of the mucous layer. These results demonstrate that L. fermentum 1.2029 has protective functions, thereby regulating the goblet cell density and the expression of mucin in the small intestine of broilers. This work also suggests that L. fermentum 1.2029 is a potential probiotic feed additive for the improvement of the intestinal function of chickens. |
| first_indexed | 2024-03-13T08:33:33Z |
| format | Article |
| id | doaj.art-106b42ab0e294fbf9aaa4e761467b780 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:33:33Z |
| publishDate | 2012-10-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-106b42ab0e294fbf9aaa4e761467b7802023-05-31T01:20:34ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862012-10-0149427328110.2141/jpsa.0110146jpsaLactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in BroilerLi Cao0Xiaojun Yang1Feifei Sun2Chanjuan Liu3Junhu Yao4College of Animal Science and Technology, Northwest A&F University, ChinaCollege of Animal Science and Technology, Northwest A&F University, ChinaCollege of Animal Science and Technology, Northwest A&F University, ChinaCollege of Animal Science and Technology, Northwest A&F University, ChinaCollege of Animal Science and Technology, Northwest A&F University, ChinaLactobacillus fermentum 1.2029 strain with high adhesion was selected for studying the mucin expression of the small intestine epithelium of broilers after the strain was orally administrated. The strain was allowed to adhere to Caco-2 and to a hydrophobic solvent to evaluate its adhesive properties. Adhesion to intestinal mucin of chickens was also tested to further characterize the in vivo adhesion of the strain. Afterward, the acid and bile salt tolerance of the strain was also evaluated. L fermentum 1.2029 showed good adhesive ability and was able to survive in low pH conditions and in the presence of bile salts. The in vivo study showed that the strain significantly increased goblet cell density in the jejunum and the level of MUC2 mRNA in both the jejunum and ileum (P<0.05). L. fermentum 1.2029 treatment didn’t affect the thickness of the mucous layer. These results demonstrate that L. fermentum 1.2029 has protective functions, thereby regulating the goblet cell density and the expression of mucin in the small intestine of broilers. This work also suggests that L. fermentum 1.2029 is a potential probiotic feed additive for the improvement of the intestinal function of chickens.https://www.jstage.jst.go.jp/article/jpsa/49/4/49_0110146/_pdf/-char/enbroiler chickensgastrointestinal tractlactobacillusmucin |
| spellingShingle | Li Cao Xiaojun Yang Feifei Sun Chanjuan Liu Junhu Yao Lactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler The Journal of Poultry Science broiler chickens gastrointestinal tract lactobacillus mucin |
| title | Lactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler |
| title_full | Lactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler |
| title_fullStr | Lactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler |
| title_full_unstemmed | Lactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler |
| title_short | Lactobacillus Strain with High Adhesion Stimulates Intestinal Mucin Expression in Broiler |
| title_sort | lactobacillus strain with high adhesion stimulates intestinal mucin expression in broiler |
| topic | broiler chickens gastrointestinal tract lactobacillus mucin |
| url | https://www.jstage.jst.go.jp/article/jpsa/49/4/49_0110146/_pdf/-char/en |
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