Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extracts
Abstract The current study aimed to identify the chemical constituents and bioactivities of the crude ethanolic extract (CEE) and its fractions (ethyl acetate (EAF), hexane (HEF), and aqueous (AEF)) from leaves of cashew (Anacardium occidentale L.) grown in Vietnam. A total of 31 compounds which bel...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.3718 |
| _version_ | 1797389254976864256 |
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| author | Dinh‐Chuong Pham Dieu‐Hien Truong Quang Huy Tran Quang Tien Ho Thieu Anh Duy Nguyen Thi Ngoc Huyen Nguyen Thanh Vinh Nguyen Thi Thao Vy Nguyen Tan Sang Cao Colin J. Barrow Hoang Chinh Nguyen |
| author_facet | Dinh‐Chuong Pham Dieu‐Hien Truong Quang Huy Tran Quang Tien Ho Thieu Anh Duy Nguyen Thi Ngoc Huyen Nguyen Thanh Vinh Nguyen Thi Thao Vy Nguyen Tan Sang Cao Colin J. Barrow Hoang Chinh Nguyen |
| author_sort | Dinh‐Chuong Pham |
| collection | DOAJ |
| description | Abstract The current study aimed to identify the chemical constituents and bioactivities of the crude ethanolic extract (CEE) and its fractions (ethyl acetate (EAF), hexane (HEF), and aqueous (AEF)) from leaves of cashew (Anacardium occidentale L.) grown in Vietnam. A total of 31 compounds which belong to alkanes, hydrocarbons, iodine, terpenoids, phenolics, and flavonoids were determined by a gas chromatography–mass spectrometry (GC–MS) analysis, with bis(2‐ethylhexyl) phthalate being the most prevailing compound. The highest total phenolic and flavonoid contents were obtained in the EAF, followed by HEF, CEE, and AQF. All samples showed promising in vitro antibacterial activity, enzyme inhibition, and anticancer activity. Among the samples tested, the EAF exhibited the highest enzyme inhibition activity against α‐amylase and α‐glucosidase (IC50 values of 51.24 μg/mL and 99.29 μg/mL, respectively), cytotoxicity activity against HeLa cells (IC50 value of 79.49 μg/mL), and antibacterial activity against Bacillus subtilis and Escherichia coli with MIC values of 5 mg/mL and 2.5 mg/mL, respectively. These findings suggest that the leaves of A. occidentale cultivated in Vietnam are a promising source of bioactive components and that EAF is a promising bioactive material warranting further pharmaceutical investigation. |
| first_indexed | 2024-03-08T22:53:12Z |
| format | Article |
| id | doaj.art-106b4eaf624040ad99fe99cd4d2e5638 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-08T22:53:12Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-106b4eaf624040ad99fe99cd4d2e56382023-12-16T07:01:19ZengWileyFood Science & Nutrition2048-71772023-12-0111127996800810.1002/fsn3.3718Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extractsDinh‐Chuong Pham0Dieu‐Hien Truong1Quang Huy Tran2Quang Tien Ho3Thieu Anh Duy Nguyen4Thi Ngoc Huyen Nguyen5Thanh Vinh Nguyen6Thi Thao Vy Nguyen7Tan Sang Cao8Colin J. Barrow9Hoang Chinh Nguyen10Faculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamFaculty of Applied Sciences Ton Duc Thang University Ho Chi Minh City VietnamCentre for Sustainable Bioproducts Deakin University Geelong Victoria AustraliaCentre for Sustainable Bioproducts Deakin University Geelong Victoria AustraliaAbstract The current study aimed to identify the chemical constituents and bioactivities of the crude ethanolic extract (CEE) and its fractions (ethyl acetate (EAF), hexane (HEF), and aqueous (AEF)) from leaves of cashew (Anacardium occidentale L.) grown in Vietnam. A total of 31 compounds which belong to alkanes, hydrocarbons, iodine, terpenoids, phenolics, and flavonoids were determined by a gas chromatography–mass spectrometry (GC–MS) analysis, with bis(2‐ethylhexyl) phthalate being the most prevailing compound. The highest total phenolic and flavonoid contents were obtained in the EAF, followed by HEF, CEE, and AQF. All samples showed promising in vitro antibacterial activity, enzyme inhibition, and anticancer activity. Among the samples tested, the EAF exhibited the highest enzyme inhibition activity against α‐amylase and α‐glucosidase (IC50 values of 51.24 μg/mL and 99.29 μg/mL, respectively), cytotoxicity activity against HeLa cells (IC50 value of 79.49 μg/mL), and antibacterial activity against Bacillus subtilis and Escherichia coli with MIC values of 5 mg/mL and 2.5 mg/mL, respectively. These findings suggest that the leaves of A. occidentale cultivated in Vietnam are a promising source of bioactive components and that EAF is a promising bioactive material warranting further pharmaceutical investigation.https://doi.org/10.1002/fsn3.3718Anacardium occidentaleantibacterialanticancerantidiabeticchemical profilingsolvent fractions |
| spellingShingle | Dinh‐Chuong Pham Dieu‐Hien Truong Quang Huy Tran Quang Tien Ho Thieu Anh Duy Nguyen Thi Ngoc Huyen Nguyen Thanh Vinh Nguyen Thi Thao Vy Nguyen Tan Sang Cao Colin J. Barrow Hoang Chinh Nguyen Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extracts Food Science & Nutrition Anacardium occidentale antibacterial anticancer antidiabetic chemical profiling solvent fractions |
| title | Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extracts |
| title_full | Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extracts |
| title_fullStr | Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extracts |
| title_full_unstemmed | Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extracts |
| title_short | Fractionation, identification of chemical constituents, and biological properties of cashew (Anacardium occidentale L.) leaf extracts |
| title_sort | fractionation identification of chemical constituents and biological properties of cashew anacardium occidentale l leaf extracts |
| topic | Anacardium occidentale antibacterial anticancer antidiabetic chemical profiling solvent fractions |
| url | https://doi.org/10.1002/fsn3.3718 |
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