In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation Sensitivity
Elevated UV-B radiation (UV-B) has been previously reported to affect plant development, physiology, and promote the biosynthesis of UV-absorbing compounds. Sweet basil (<i>Ocimum basilicum</i> L.) is an aromatic herb, widely cultivated worldwide for its use in the food, pharmaceuticals,...
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MDPI AG
2021-05-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/5/101 |
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| author | Haana Mosadegh Alice Trivellini Rita Maggini Antonio Ferrante Luca Incrocci Anna Mensuali |
| author_facet | Haana Mosadegh Alice Trivellini Rita Maggini Antonio Ferrante Luca Incrocci Anna Mensuali |
| author_sort | Haana Mosadegh |
| collection | DOAJ |
| description | Elevated UV-B radiation (UV-B) has been previously reported to affect plant development, physiology, and promote the biosynthesis of UV-absorbing compounds. Sweet basil (<i>Ocimum basilicum</i> L.) is an aromatic herb, widely cultivated worldwide for its use in the food, pharmaceuticals, and cosmetics industry. This species exhibits high diversity among different ecotypes based on their geographical locations. There has been little research on intra-specific photosynthetic and metabolic differences in UV-B tolerance across ecotypes from different geographical areas. This study evaluated the protection responses to high UV-B radiation of nine <i>O. basilicum</i> accessions with different geographic origins. Specifically, the changes in chlorophyll <i>a</i> fluorescence parameters and the leaf rosmarinic acid (RA) compound were assessed using an “in vivo-vitro system” in a closed-type plant production system. Our results revealed a significant variation in UV-B protection mechanisms among accessions when plants were treated with high UV-B doses. The accumulation of RA increased significantly by UV-B light treatment in OCI142, OCI148, OCI30, OCI160, and OCI102, with the highest concentration measured in OCI160 plants. This ecotype showed the highest value of the Fv/Fm ratio, 0.70, after 48 h. Recovery of leaf functionality was more rapid in OCI160 than in other sweet basil accessions, which may indicate better photosynthetic capacity associated with enhanced biosynthesis of UV absorbing compounds. This study shows that the biosynthesis of the UV-absorbing compound (RA) represents an effective mechanism to reduce the photoinhibitory and photooxidative damage caused by high UV stress. |
| first_indexed | 2024-03-10T11:37:12Z |
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| id | doaj.art-106b5ac511c943d6b8bf74ce46b9f24e |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T11:37:12Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-106b5ac511c943d6b8bf74ce46b9f24e2023-11-21T18:42:50ZengMDPI AGHorticulturae2311-75242021-05-017510110.3390/horticulturae7050101In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation SensitivityHaana Mosadegh0Alice Trivellini1Rita Maggini2Antonio Ferrante3Luca Incrocci4Anna Mensuali5Institute of Life Sciences, Scuola Superiore Sant’Anna, Pz. Martiri della Libertà 33, 56127 Pisa, ItalyInstitute of Life Sciences, Scuola Superiore Sant’Anna, Pz. Martiri della Libertà 33, 56127 Pisa, ItalyDepartment of Agriculture, Food and Environment, Università Degli Studi di Pisa, Via del Borghetto 80, 56124 Pisa, ItalyDepartment of Agricultural and Environmental Sciences, Università degli Studi di Milano, 20133 Milano, ItalyDepartment of Agriculture, Food and Environment, Università Degli Studi di Pisa, Via del Borghetto 80, 56124 Pisa, ItalyInstitute of Life Sciences, Scuola Superiore Sant’Anna, Pz. Martiri della Libertà 33, 56127 Pisa, ItalyElevated UV-B radiation (UV-B) has been previously reported to affect plant development, physiology, and promote the biosynthesis of UV-absorbing compounds. Sweet basil (<i>Ocimum basilicum</i> L.) is an aromatic herb, widely cultivated worldwide for its use in the food, pharmaceuticals, and cosmetics industry. This species exhibits high diversity among different ecotypes based on their geographical locations. There has been little research on intra-specific photosynthetic and metabolic differences in UV-B tolerance across ecotypes from different geographical areas. This study evaluated the protection responses to high UV-B radiation of nine <i>O. basilicum</i> accessions with different geographic origins. Specifically, the changes in chlorophyll <i>a</i> fluorescence parameters and the leaf rosmarinic acid (RA) compound were assessed using an “in vivo-vitro system” in a closed-type plant production system. Our results revealed a significant variation in UV-B protection mechanisms among accessions when plants were treated with high UV-B doses. The accumulation of RA increased significantly by UV-B light treatment in OCI142, OCI148, OCI30, OCI160, and OCI102, with the highest concentration measured in OCI160 plants. This ecotype showed the highest value of the Fv/Fm ratio, 0.70, after 48 h. Recovery of leaf functionality was more rapid in OCI160 than in other sweet basil accessions, which may indicate better photosynthetic capacity associated with enhanced biosynthesis of UV absorbing compounds. This study shows that the biosynthesis of the UV-absorbing compound (RA) represents an effective mechanism to reduce the photoinhibitory and photooxidative damage caused by high UV stress.https://www.mdpi.com/2311-7524/7/5/101UV-B stressphotosynthetic performanceUV-B screening compoundsbasiltissue culture |
| spellingShingle | Haana Mosadegh Alice Trivellini Rita Maggini Antonio Ferrante Luca Incrocci Anna Mensuali In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation Sensitivity Horticulturae UV-B stress photosynthetic performance UV-B screening compounds basil tissue culture |
| title | In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation Sensitivity |
| title_full | In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation Sensitivity |
| title_fullStr | In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation Sensitivity |
| title_full_unstemmed | In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation Sensitivity |
| title_short | In-Vivo In-Vitro Screening of <i>Ocimum basilicum</i> L. Ecotypes with Differential UV-B Radiation Sensitivity |
| title_sort | in vivo in vitro screening of i ocimum basilicum i l ecotypes with differential uv b radiation sensitivity |
| topic | UV-B stress photosynthetic performance UV-B screening compounds basil tissue culture |
| url | https://www.mdpi.com/2311-7524/7/5/101 |
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