Patterns of expression of cell wall related genes in sugarcane
Our search for genes related to cell wall metabolism in the sugarcane expressed sequence tag (SUCEST) database (<A HREF="http://sucest.lbi.dcc.unicamp.br/">http://sucest.lbi.dcc.unicamp.br</A>) resulted in 3,283 reads (1% of the total reads) which were grouped into 459 clusters...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2001-01-01
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| Series: | Genetics and Molecular Biology |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100026 |
| _version_ | 1819160650377592832 |
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| author | Lima D.U. Santos H.P. Tiné M.A. Molle F.R.D. Buckeridge M.S. |
| author_facet | Lima D.U. Santos H.P. Tiné M.A. Molle F.R.D. Buckeridge M.S. |
| author_sort | Lima D.U. |
| collection | DOAJ |
| description | Our search for genes related to cell wall metabolism in the sugarcane expressed sequence tag (SUCEST) database (<A HREF="http://sucest.lbi.dcc.unicamp.br/">http://sucest.lbi.dcc.unicamp.br</A>) resulted in 3,283 reads (1% of the total reads) which were grouped into 459 clusters (potential genes) with an average of 7.1 reads per cluster. To more clearly display our correlation coefficients, we constructed surface maps which we used to investigate the relationship between cell wall genes and the sugarcane tissues libraries from which they came. The only significant correlations that we found between cell wall genes and/or their expression within particular libraries were neutral or synergetic. Genes related to cellulose biosynthesis were from the CesA family, and were found to be the most abundant cell wall related genes in the SUCEST database. We found that the highest number of CesA reads came from the root and stem libraries. The genes with the greatest number of reads were those involved in cell wall hydrolases (e.g. beta-1,3-glucanases, xyloglucan endo-beta-transglycosylase, beta-glucosidase and endo-beta-mannanase). Correlation analyses by surface mapping revealed that the expression of genes related to biosynthesis seems to be associated with the hydrolysis of hemicelluloses, pectin hydrolases being mainly associated with xyloglucan hydrolases. The patterns of cell wall related gene expression in sugarcane based on the number of reads per cluster reflected quite well the expected physiological characteristics of the tissues. This is the first work to provide a general view on plant cell wall metabolism through the expression of related genes in almost all the tissues of a plant at the same time. For example, developing flowers behaved similarly to both meristematic tissues and leaf-root transition zone tissues. Besides providing a basis for future research on the mechanisms of plant development which involve the cell wall, our findings will provide valuable tools for plant engineering in the near future. |
| first_indexed | 2024-12-22T16:59:49Z |
| format | Article |
| id | doaj.art-106e36b00d164071a53c0cbed15fd7e6 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T16:59:49Z |
| publishDate | 2001-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-106e36b00d164071a53c0cbed15fd7e62022-12-21T18:19:21ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852001-01-01241-4191198Patterns of expression of cell wall related genes in sugarcaneLima D.U.Santos H.P.Tiné M.A.Molle F.R.D.Buckeridge M.S.Our search for genes related to cell wall metabolism in the sugarcane expressed sequence tag (SUCEST) database (<A HREF="http://sucest.lbi.dcc.unicamp.br/">http://sucest.lbi.dcc.unicamp.br</A>) resulted in 3,283 reads (1% of the total reads) which were grouped into 459 clusters (potential genes) with an average of 7.1 reads per cluster. To more clearly display our correlation coefficients, we constructed surface maps which we used to investigate the relationship between cell wall genes and the sugarcane tissues libraries from which they came. The only significant correlations that we found between cell wall genes and/or their expression within particular libraries were neutral or synergetic. Genes related to cellulose biosynthesis were from the CesA family, and were found to be the most abundant cell wall related genes in the SUCEST database. We found that the highest number of CesA reads came from the root and stem libraries. The genes with the greatest number of reads were those involved in cell wall hydrolases (e.g. beta-1,3-glucanases, xyloglucan endo-beta-transglycosylase, beta-glucosidase and endo-beta-mannanase). Correlation analyses by surface mapping revealed that the expression of genes related to biosynthesis seems to be associated with the hydrolysis of hemicelluloses, pectin hydrolases being mainly associated with xyloglucan hydrolases. The patterns of cell wall related gene expression in sugarcane based on the number of reads per cluster reflected quite well the expected physiological characteristics of the tissues. This is the first work to provide a general view on plant cell wall metabolism through the expression of related genes in almost all the tissues of a plant at the same time. For example, developing flowers behaved similarly to both meristematic tissues and leaf-root transition zone tissues. Besides providing a basis for future research on the mechanisms of plant development which involve the cell wall, our findings will provide valuable tools for plant engineering in the near future.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100026 |
| spellingShingle | Lima D.U. Santos H.P. Tiné M.A. Molle F.R.D. Buckeridge M.S. Patterns of expression of cell wall related genes in sugarcane Genetics and Molecular Biology |
| title | Patterns of expression of cell wall related genes in sugarcane |
| title_full | Patterns of expression of cell wall related genes in sugarcane |
| title_fullStr | Patterns of expression of cell wall related genes in sugarcane |
| title_full_unstemmed | Patterns of expression of cell wall related genes in sugarcane |
| title_short | Patterns of expression of cell wall related genes in sugarcane |
| title_sort | patterns of expression of cell wall related genes in sugarcane |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100026 |
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