Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot Growth
Pyramidal-, erect- or upright-growing plant forms are characterized by narrow branch angles of shoots and leaves. The putative advantage of upright-leaf and shoot habit could be a more efficient penetration of light into lower canopy layers. Pyramidal genotypes have already been reported for various...
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MDPI AG
2021-11-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/12/12/1615 |
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| author | Matthias Fladung |
| author_facet | Matthias Fladung |
| author_sort | Matthias Fladung |
| collection | DOAJ |
| description | Pyramidal-, erect- or upright-growing plant forms are characterized by narrow branch angles of shoots and leaves. The putative advantage of upright-leaf and shoot habit could be a more efficient penetration of light into lower canopy layers. Pyramidal genotypes have already been reported for various tree genotypes including peach. The paralogous rice ortholog <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) has been proposed to be the responsible gene for upright growth. However, it has not really been demonstrated for any of the pyramidal tree genotypes that a knock-out mutation of the <i>TAC1</i> gene is causing pyramidal plant growth. By in silico analyses, we have identified a putative rice <i>TAC1</i> ortholog (Potri.014G102600, “TAC-14”) and its paralog (Potri.002G175300, “TAC-2”) in the genome of <i>P. trichocarpa</i>. Two putative <i>PcTAC1</i> orthologs in the <i>P. × canescens</i> clone INRA 717-1B4 were successfully knocked-out by applying a transgenic CRISPR/Cas9-approach. The mutants were molecularly analyzed and phenotyped over a period of three years in a glasshouse. Our results indicate that the homozygous knock-out of “TAC-14” is sufficient to induce pyramidal plant growth in <i>P. × canescens</i>. If up to twice as many pyramidal individuals were planted on short rotation coppices (SRCs), this could lead to higher wood yield, without any breeding, simply by increasing the number of trees on a default field size. |
| first_indexed | 2024-03-10T04:06:54Z |
| format | Article |
| id | doaj.art-106f1d25d8d243629e3b39f3d7d25c02 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T04:06:54Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-106f1d25d8d243629e3b39f3d7d25c022023-11-23T08:19:58ZengMDPI AGForests1999-49072021-11-011212161510.3390/f12121615Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot GrowthMatthias Fladung0Thünen Institute of Forest Genetics, Sieker Landstrasse 2, 22927 Grosshansdorf, GermanyPyramidal-, erect- or upright-growing plant forms are characterized by narrow branch angles of shoots and leaves. The putative advantage of upright-leaf and shoot habit could be a more efficient penetration of light into lower canopy layers. Pyramidal genotypes have already been reported for various tree genotypes including peach. The paralogous rice ortholog <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) has been proposed to be the responsible gene for upright growth. However, it has not really been demonstrated for any of the pyramidal tree genotypes that a knock-out mutation of the <i>TAC1</i> gene is causing pyramidal plant growth. By in silico analyses, we have identified a putative rice <i>TAC1</i> ortholog (Potri.014G102600, “TAC-14”) and its paralog (Potri.002G175300, “TAC-2”) in the genome of <i>P. trichocarpa</i>. Two putative <i>PcTAC1</i> orthologs in the <i>P. × canescens</i> clone INRA 717-1B4 were successfully knocked-out by applying a transgenic CRISPR/Cas9-approach. The mutants were molecularly analyzed and phenotyped over a period of three years in a glasshouse. Our results indicate that the homozygous knock-out of “TAC-14” is sufficient to induce pyramidal plant growth in <i>P. × canescens</i>. If up to twice as many pyramidal individuals were planted on short rotation coppices (SRCs), this could lead to higher wood yield, without any breeding, simply by increasing the number of trees on a default field size.https://www.mdpi.com/1999-4907/12/12/1615CRISPR/Cas9genome editing<i>Populus</i>INRA 717-1B4pyramidal plant habitusleaf petiole angle |
| spellingShingle | Matthias Fladung Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot Growth Forests CRISPR/Cas9 genome editing <i>Populus</i> INRA 717-1B4 pyramidal plant habitus leaf petiole angle |
| title | Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot Growth |
| title_full | Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot Growth |
| title_fullStr | Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot Growth |
| title_full_unstemmed | Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot Growth |
| title_short | Targeted CRISPR/Cas9-Based Knock-Out of the Rice Orthologs <i>TILLER ANGLE CONTROL 1</i> (<i>TAC1</i>) in Poplar Induces Erect Leaf Habit and Shoot Growth |
| title_sort | targeted crispr cas9 based knock out of the rice orthologs i tiller angle control 1 i i tac1 i in poplar induces erect leaf habit and shoot growth |
| topic | CRISPR/Cas9 genome editing <i>Populus</i> INRA 717-1B4 pyramidal plant habitus leaf petiole angle |
| url | https://www.mdpi.com/1999-4907/12/12/1615 |
| work_keys_str_mv | AT matthiasfladung targetedcrisprcas9basedknockoutofthericeorthologsitilleranglecontrol1iitac1iinpoplarinduceserectleafhabitandshootgrowth |