Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California
Abstract Background Homalodisca vitripennis Germar, the glassy-winged sharpshooter, is an invasive insect in California and a critical threat to agriculture through its transmission of the plant pathogen, Xylella fastidiosa. Quarantine, broad-spectrum insecticides, and biological control have been u...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-08939-1 |
| _version_ | 1811250026395992064 |
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| author | Cassandra L. Ettinger Frank J. Byrne Inaiara de Souza Pacheco Dylan J. Brown Linda L. Walling Peter W. Atkinson Richard A. Redak Jason E. Stajich |
| author_facet | Cassandra L. Ettinger Frank J. Byrne Inaiara de Souza Pacheco Dylan J. Brown Linda L. Walling Peter W. Atkinson Richard A. Redak Jason E. Stajich |
| author_sort | Cassandra L. Ettinger |
| collection | DOAJ |
| description | Abstract Background Homalodisca vitripennis Germar, the glassy-winged sharpshooter, is an invasive insect in California and a critical threat to agriculture through its transmission of the plant pathogen, Xylella fastidiosa. Quarantine, broad-spectrum insecticides, and biological control have been used for population management of H. vitripennis since its invasion and subsequent proliferation throughout California. Recently wide-spread neonicotinoid resistance has been detected in populations of H. vitripennis in the southern portions of California’s Central Valley. In order to better understand potential mechanisms of H. vitripennis neonicotinoid resistance, we performed RNA sequencing on wild-caught insecticide-resistant and relatively susceptible sharpshooters to profile their transcriptome and population structure. Results We identified 81 differentially expressed genes with higher expression in resistant individuals. The significant largest differentially expressed candidate gene linked to resistance status was a cytochrome P450 gene with similarity to CYP6A9. Furthermore, we observed an over-enrichment of GO terms representing functions supportive of roles in resistance mechanisms (cytochrome P450s, M13 peptidases, and cuticle structural proteins). Finally, we saw no evidence of broad-scale population structure, perhaps due to H. vitripennis' relatively recent introduction to California or due to the relatively small geographic scale investigated here. Conclusions In this work, we characterized the transcriptome of insecticide-resistant and susceptible H. vitripennis and identified candidate genes that may be involved in resistance mechanisms for this species. Future work should seek to build on the transcriptome profiling performed here to confirm the role of the identified genes, particularly the cytochrome P450, in resistance in H. vitripennis. We hope this work helps aid future population management strategies for this and other species with growing insecticide resistance. |
| first_indexed | 2024-04-12T15:56:49Z |
| format | Article |
| id | doaj.art-10701897530b424187c1369d1b3f2e66 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T15:56:49Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-10701897530b424187c1369d1b3f2e662022-12-22T03:26:19ZengBMCBMC Genomics1471-21642022-10-0123111310.1186/s12864-022-08939-1Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern CaliforniaCassandra L. Ettinger0Frank J. Byrne1Inaiara de Souza Pacheco2Dylan J. Brown3Linda L. Walling4Peter W. Atkinson5Richard A. Redak6Jason E. Stajich7Department of Microbiology and Plant Pathology, University of CaliforniaDepartment of Entomology, University of CaliforniaDepartment of Entomology, University of CaliforniaDepartment of Entomology, University of CaliforniaDepartment of Botany and Plant Sciences, University of CaliforniaDepartment of Entomology, University of CaliforniaDepartment of Entomology, University of CaliforniaDepartment of Microbiology and Plant Pathology, University of CaliforniaAbstract Background Homalodisca vitripennis Germar, the glassy-winged sharpshooter, is an invasive insect in California and a critical threat to agriculture through its transmission of the plant pathogen, Xylella fastidiosa. Quarantine, broad-spectrum insecticides, and biological control have been used for population management of H. vitripennis since its invasion and subsequent proliferation throughout California. Recently wide-spread neonicotinoid resistance has been detected in populations of H. vitripennis in the southern portions of California’s Central Valley. In order to better understand potential mechanisms of H. vitripennis neonicotinoid resistance, we performed RNA sequencing on wild-caught insecticide-resistant and relatively susceptible sharpshooters to profile their transcriptome and population structure. Results We identified 81 differentially expressed genes with higher expression in resistant individuals. The significant largest differentially expressed candidate gene linked to resistance status was a cytochrome P450 gene with similarity to CYP6A9. Furthermore, we observed an over-enrichment of GO terms representing functions supportive of roles in resistance mechanisms (cytochrome P450s, M13 peptidases, and cuticle structural proteins). Finally, we saw no evidence of broad-scale population structure, perhaps due to H. vitripennis' relatively recent introduction to California or due to the relatively small geographic scale investigated here. Conclusions In this work, we characterized the transcriptome of insecticide-resistant and susceptible H. vitripennis and identified candidate genes that may be involved in resistance mechanisms for this species. Future work should seek to build on the transcriptome profiling performed here to confirm the role of the identified genes, particularly the cytochrome P450, in resistance in H. vitripennis. We hope this work helps aid future population management strategies for this and other species with growing insecticide resistance.https://doi.org/10.1186/s12864-022-08939-1Glassy-winged sharpshooterInsecticide resistanceTranscriptomeRNA-seqHomalodisca vitripennisNeonicotinoids |
| spellingShingle | Cassandra L. Ettinger Frank J. Byrne Inaiara de Souza Pacheco Dylan J. Brown Linda L. Walling Peter W. Atkinson Richard A. Redak Jason E. Stajich Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California BMC Genomics Glassy-winged sharpshooter Insecticide resistance Transcriptome RNA-seq Homalodisca vitripennis Neonicotinoids |
| title | Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California |
| title_full | Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California |
| title_fullStr | Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California |
| title_full_unstemmed | Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California |
| title_short | Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California |
| title_sort | transcriptome and population structure of glassy winged sharpshooters homalodisca vitripennis with varying insecticide resistance in southern california |
| topic | Glassy-winged sharpshooter Insecticide resistance Transcriptome RNA-seq Homalodisca vitripennis Neonicotinoids |
| url | https://doi.org/10.1186/s12864-022-08939-1 |
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