Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics
To understand the brain, molecular details need to be overlaid onto neural wiring diagrams so that synaptic mode, neuromodulation and critical signaling operations can be considered. Single-cell transcriptomics provide a unique opportunity to collect this information. Here we present an initial anal...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/34550 |
| _version_ | 1811253348277420032 |
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| author | Vincent Croset Christoph D Treiber Scott Waddell |
| author_facet | Vincent Croset Christoph D Treiber Scott Waddell |
| author_sort | Vincent Croset |
| collection | DOAJ |
| description | To understand the brain, molecular details need to be overlaid onto neural wiring diagrams so that synaptic mode, neuromodulation and critical signaling operations can be considered. Single-cell transcriptomics provide a unique opportunity to collect this information. Here we present an initial analysis of thousands of individual cells from Drosophila midbrain, that were acquired using Drop-Seq. A number of approaches permitted the assignment of transcriptional profiles to several major brain regions and cell-types. Expression of biosynthetic enzymes and reuptake mechanisms allows all the neurons to be typed according to the neurotransmitter or neuromodulator that they produce and presumably release. Some neuropeptides are preferentially co-expressed in neurons using a particular fast-acting transmitter, or monoamine. Neuromodulatory and neurotransmitter receptor subunit expression illustrates the potential of these molecules in generating complexity in neural circuit function. This cell atlas dataset provides an important resource to link molecular operations to brain regions and complex neural processes. |
| first_indexed | 2024-04-12T16:49:33Z |
| format | Article |
| id | doaj.art-1073f5e57c4f41c2947ab04a891f9e3e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:49:33Z |
| publishDate | 2018-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1073f5e57c4f41c2947ab04a891f9e3e2022-12-22T03:24:26ZengeLife Sciences Publications LtdeLife2050-084X2018-04-01710.7554/eLife.34550Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomicsVincent Croset0Christoph D Treiber1https://orcid.org/0000-0002-6994-091XScott Waddell2https://orcid.org/0000-0003-4503-6229Centre for Neural Circuits and Behaviour, The University of Oxford, Oxford, United KingdomCentre for Neural Circuits and Behaviour, The University of Oxford, Oxford, United KingdomCentre for Neural Circuits and Behaviour, The University of Oxford, Oxford, United KingdomTo understand the brain, molecular details need to be overlaid onto neural wiring diagrams so that synaptic mode, neuromodulation and critical signaling operations can be considered. Single-cell transcriptomics provide a unique opportunity to collect this information. Here we present an initial analysis of thousands of individual cells from Drosophila midbrain, that were acquired using Drop-Seq. A number of approaches permitted the assignment of transcriptional profiles to several major brain regions and cell-types. Expression of biosynthetic enzymes and reuptake mechanisms allows all the neurons to be typed according to the neurotransmitter or neuromodulator that they produce and presumably release. Some neuropeptides are preferentially co-expressed in neurons using a particular fast-acting transmitter, or monoamine. Neuromodulatory and neurotransmitter receptor subunit expression illustrates the potential of these molecules in generating complexity in neural circuit function. This cell atlas dataset provides an important resource to link molecular operations to brain regions and complex neural processes.https://elifesciences.org/articles/34550single-cell sequencingDrosophila braincellular diversityneurotransmittersneuropeptidesneuromodulation |
| spellingShingle | Vincent Croset Christoph D Treiber Scott Waddell Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics eLife single-cell sequencing Drosophila brain cellular diversity neurotransmitters neuropeptides neuromodulation |
| title | Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics |
| title_full | Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics |
| title_fullStr | Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics |
| title_full_unstemmed | Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics |
| title_short | Cellular diversity in the Drosophila midbrain revealed by single-cell transcriptomics |
| title_sort | cellular diversity in the drosophila midbrain revealed by single cell transcriptomics |
| topic | single-cell sequencing Drosophila brain cellular diversity neurotransmitters neuropeptides neuromodulation |
| url | https://elifesciences.org/articles/34550 |
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