Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes
Heart Failure (HF) is a syndrome, which implies the existence of different phenotypes. The new categorization includes patients with preserved ejection fraction (HFpEF), mid-range EF (HFmrEF), and reduced EF (HFrEF) but the molecular mechanisms involved in these HF phenotypes have not yet been exhau...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/11/1590 |
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| author | Valeria Conti Graziamaria Corbi Maria Vincenza Polito Michele Ciccarelli Valentina Manzo Martina Torsiello Emanuela De Bellis Federica D’Auria Gennaro Vitulano Federico Piscione Albino Carrizzo Paola Di Pietro Carmine Vecchione Nicola Ferrara Amelia Filippelli |
| author_facet | Valeria Conti Graziamaria Corbi Maria Vincenza Polito Michele Ciccarelli Valentina Manzo Martina Torsiello Emanuela De Bellis Federica D’Auria Gennaro Vitulano Federico Piscione Albino Carrizzo Paola Di Pietro Carmine Vecchione Nicola Ferrara Amelia Filippelli |
| author_sort | Valeria Conti |
| collection | DOAJ |
| description | Heart Failure (HF) is a syndrome, which implies the existence of different phenotypes. The new categorization includes patients with preserved ejection fraction (HFpEF), mid-range EF (HFmrEF), and reduced EF (HFrEF) but the molecular mechanisms involved in these HF phenotypes have not yet been exhaustively investigated. Sirt1 plays a crucial role in biological processes strongly related to HF. This study aimed to evaluate whether Sirt1 activity was correlated with EF and other parameters in HFpEF, HFmrEF, and HFrEF. Seventy patients, HFpEF (<i>n</i> = 23), HFmrEF (<i>n</i> = 23) and HFrEF (<i>n</i> = 24), were enrolled at the Cardiology Unit of the University Hospital of Salerno. Sirt1 activity was measured in peripheral blood mononuclear cells (PBMCs). Angiotensin-Converting Enzyme 2 (ACE2) activity, Tumor Necrosis Factor-alpha (TNF-α) and Brain Natriuretic Peptide (BNP) levels were quantified in plasma. HFpEF showed lower Sirt1 and ACE2 activities than both HFmrEF and HFrEF (<i>p</i> < 0.0001), without difference compared to No HF controls. In HFmrEF and HFrEF a very strong correlation was found between Sirt1 activity and EF (r<sup>2</sup> = 0.899 and r<sup>2</sup> = 0.909, respectively), and between ACE2 activity and Sirt1 (r<sup>2</sup> = 0.801 and r<sup>2</sup> = 0.802, respectively). HFrEF showed the highest TNF-α levels without reaching statistical significance. Significant differences in BNP were found among the groups, with the highest levels in the HFrEF. Determining Sirt1 activity in PBMCs is useful to distinguish the HF patients’ phenotypes from each other, especially HFmrEF/HFrEF from HFpEF. |
| first_indexed | 2024-03-10T14:37:33Z |
| format | Article |
| id | doaj.art-107747d6c8b347b2878ae76719ed8bd0 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T14:37:33Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-107747d6c8b347b2878ae76719ed8bd02023-11-20T22:03:13ZengMDPI AGBiomolecules2218-273X2020-11-011011159010.3390/biom10111590Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure PhenotypesValeria Conti0Graziamaria Corbi1Maria Vincenza Polito2Michele Ciccarelli3Valentina Manzo4Martina Torsiello5Emanuela De Bellis6Federica D’Auria7Gennaro Vitulano8Federico Piscione9Albino Carrizzo10Paola Di Pietro11Carmine Vecchione12Nicola Ferrara13Amelia Filippelli14Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine and Health Sciences, University of Molise, 86100 Campobasso, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyDepartment of Translational Medical Sciences, Federico II University of Naples, 80131 Naples, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, 84081 Baronissi, ItalyHeart Failure (HF) is a syndrome, which implies the existence of different phenotypes. The new categorization includes patients with preserved ejection fraction (HFpEF), mid-range EF (HFmrEF), and reduced EF (HFrEF) but the molecular mechanisms involved in these HF phenotypes have not yet been exhaustively investigated. Sirt1 plays a crucial role in biological processes strongly related to HF. This study aimed to evaluate whether Sirt1 activity was correlated with EF and other parameters in HFpEF, HFmrEF, and HFrEF. Seventy patients, HFpEF (<i>n</i> = 23), HFmrEF (<i>n</i> = 23) and HFrEF (<i>n</i> = 24), were enrolled at the Cardiology Unit of the University Hospital of Salerno. Sirt1 activity was measured in peripheral blood mononuclear cells (PBMCs). Angiotensin-Converting Enzyme 2 (ACE2) activity, Tumor Necrosis Factor-alpha (TNF-α) and Brain Natriuretic Peptide (BNP) levels were quantified in plasma. HFpEF showed lower Sirt1 and ACE2 activities than both HFmrEF and HFrEF (<i>p</i> < 0.0001), without difference compared to No HF controls. In HFmrEF and HFrEF a very strong correlation was found between Sirt1 activity and EF (r<sup>2</sup> = 0.899 and r<sup>2</sup> = 0.909, respectively), and between ACE2 activity and Sirt1 (r<sup>2</sup> = 0.801 and r<sup>2</sup> = 0.802, respectively). HFrEF showed the highest TNF-α levels without reaching statistical significance. Significant differences in BNP were found among the groups, with the highest levels in the HFrEF. Determining Sirt1 activity in PBMCs is useful to distinguish the HF patients’ phenotypes from each other, especially HFmrEF/HFrEF from HFpEF.https://www.mdpi.com/2218-273X/10/11/1590heart failure with preserved ejection fractionheart failure with mid-range ejection fractionheart failure with reduced ejection fractionsirtuinsSirt1 |
| spellingShingle | Valeria Conti Graziamaria Corbi Maria Vincenza Polito Michele Ciccarelli Valentina Manzo Martina Torsiello Emanuela De Bellis Federica D’Auria Gennaro Vitulano Federico Piscione Albino Carrizzo Paola Di Pietro Carmine Vecchione Nicola Ferrara Amelia Filippelli Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes Biomolecules heart failure with preserved ejection fraction heart failure with mid-range ejection fraction heart failure with reduced ejection fraction sirtuins Sirt1 |
| title | Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes |
| title_full | Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes |
| title_fullStr | Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes |
| title_full_unstemmed | Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes |
| title_short | Sirt1 Activity in PBMCs as a Biomarker of Different Heart Failure Phenotypes |
| title_sort | sirt1 activity in pbmcs as a biomarker of different heart failure phenotypes |
| topic | heart failure with preserved ejection fraction heart failure with mid-range ejection fraction heart failure with reduced ejection fraction sirtuins Sirt1 |
| url | https://www.mdpi.com/2218-273X/10/11/1590 |
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