Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling Pathway
Low-molecular-weight chitosan (LMWC), a product of chitosan deacetylation, possesses anti-inflammatory effects. In the present study, a porcine small intestinal epithelial cell line, IPEC-J2, was used to assess the protective effects of LMWC on lipopolysaccharide (LPS)-induced intestinal epithelial...
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/3/569 |
| _version_ | 1797408416433438720 |
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| author | Jiao Zhang Jin Wan Daiwen Chen Bing Yu Jun He |
| author_facet | Jiao Zhang Jin Wan Daiwen Chen Bing Yu Jun He |
| author_sort | Jiao Zhang |
| collection | DOAJ |
| description | Low-molecular-weight chitosan (LMWC), a product of chitosan deacetylation, possesses anti-inflammatory effects. In the present study, a porcine small intestinal epithelial cell line, IPEC-J2, was used to assess the protective effects of LMWC on lipopolysaccharide (LPS)-induced intestinal epithelial cell injury. IPEC-J2 cells were pretreated with or without LMWC (400 μg/mL) in the presence or absence of LPS (5 μg/mL) for 6 h. LMWC pretreatment increased (<i>p</i> < 0.05) the occludin abundance and decreased (<i>p</i> < 0.05) the tumour necrosis factor-α (TNF-α) production, apoptosis rate and cleaved cysteinyl aspartate-specific protease-3 (caspase-3) and -8 contents in LPS-treated IPEC-J2 cells. Moreover, LMWC pretreatment downregulated (<i>p</i> < 0.05) the expression levels of TNF receptor 1 (<i>TNFR1</i>) and TNFR-associated death domain and decreased (<i>p</i> < 0.05) the nuclear and cytoplasmic abundance of nuclear factor-κB (NF-κB) p65 in LPS-stimulated IPEC-J2 cells. These results suggest that LMWC exerts a mitigation effect on LPS-induced intestinal epithelial cell damage by suppressing TNFR1-mediated apoptosis and decreasing the production of proinflammatory cytokines via the inhibition of NF-κB signalling pathway. |
| first_indexed | 2024-03-09T03:58:10Z |
| format | Article |
| id | doaj.art-1078797a51a7403a8e4313b70c30d4c1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T03:58:10Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1078797a51a7403a8e4313b70c30d4c12023-12-03T14:16:34ZengMDPI AGMolecules1420-30492021-01-0126356910.3390/molecules26030569Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling PathwayJiao Zhang0Jin Wan1Daiwen Chen2Bing Yu3Jun He4Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, ChinaLow-molecular-weight chitosan (LMWC), a product of chitosan deacetylation, possesses anti-inflammatory effects. In the present study, a porcine small intestinal epithelial cell line, IPEC-J2, was used to assess the protective effects of LMWC on lipopolysaccharide (LPS)-induced intestinal epithelial cell injury. IPEC-J2 cells were pretreated with or without LMWC (400 μg/mL) in the presence or absence of LPS (5 μg/mL) for 6 h. LMWC pretreatment increased (<i>p</i> < 0.05) the occludin abundance and decreased (<i>p</i> < 0.05) the tumour necrosis factor-α (TNF-α) production, apoptosis rate and cleaved cysteinyl aspartate-specific protease-3 (caspase-3) and -8 contents in LPS-treated IPEC-J2 cells. Moreover, LMWC pretreatment downregulated (<i>p</i> < 0.05) the expression levels of TNF receptor 1 (<i>TNFR1</i>) and TNFR-associated death domain and decreased (<i>p</i> < 0.05) the nuclear and cytoplasmic abundance of nuclear factor-κB (NF-κB) p65 in LPS-stimulated IPEC-J2 cells. These results suggest that LMWC exerts a mitigation effect on LPS-induced intestinal epithelial cell damage by suppressing TNFR1-mediated apoptosis and decreasing the production of proinflammatory cytokines via the inhibition of NF-κB signalling pathway.https://www.mdpi.com/1420-3049/26/3/569low-molecular-weight chitosanlipopolysaccharideinflammatory injurycell apoptosisintestinal epithelial cells |
| spellingShingle | Jiao Zhang Jin Wan Daiwen Chen Bing Yu Jun He Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling Pathway Molecules low-molecular-weight chitosan lipopolysaccharide inflammatory injury cell apoptosis intestinal epithelial cells |
| title | Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling Pathway |
| title_full | Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling Pathway |
| title_fullStr | Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling Pathway |
| title_full_unstemmed | Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling Pathway |
| title_short | Low-Molecular-Weight Chitosan Attenuates Lipopolysaccharide-Induced Inflammation in IPEC-J2 Cells by Inhibiting the Nuclear Factor-κB Signalling Pathway |
| title_sort | low molecular weight chitosan attenuates lipopolysaccharide induced inflammation in ipec j2 cells by inhibiting the nuclear factor κb signalling pathway |
| topic | low-molecular-weight chitosan lipopolysaccharide inflammatory injury cell apoptosis intestinal epithelial cells |
| url | https://www.mdpi.com/1420-3049/26/3/569 |
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