Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma
Oral squamous cell carcinoma (OSCC), one of the most common malignant tumors of the head and neck, is closely associated with the presence of oral microbes. However, the microbiomes of different oral niches in OSCC patients and their association with OSCC have not been adequately characterized. In t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.905653/full |
| _version_ | 1818083959702028288 |
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| author | Fujiao Nie Fujiao Nie Lihua Wang Lihua Wang Yingying Huang Yingying Huang Pishan Yang Pishan Yang Pizhang Gong Pizhang Gong Qiang Feng Qiang Feng Chengzhe Yang Chengzhe Yang |
| author_facet | Fujiao Nie Fujiao Nie Lihua Wang Lihua Wang Yingying Huang Yingying Huang Pishan Yang Pishan Yang Pizhang Gong Pizhang Gong Qiang Feng Qiang Feng Chengzhe Yang Chengzhe Yang |
| author_sort | Fujiao Nie |
| collection | DOAJ |
| description | Oral squamous cell carcinoma (OSCC), one of the most common malignant tumors of the head and neck, is closely associated with the presence of oral microbes. However, the microbiomes of different oral niches in OSCC patients and their association with OSCC have not been adequately characterized. In this study, 305 samples were collected from 65 OSCC patients, including tumor tissue, adjacent normal tissue (paracancerous tissue), cancer surface tissue, anatomically matched contralateral normal mucosa, saliva, and tongue coat. 16S ribosomal DNA (16S rDNA) sequencing was used to compare the microbial composition, distribution, and co-occurrence network of different oral niches. The association between the microbiome and the clinical features of OSCC was also characterized. The oral microbiome of OSCC patients showed a regular ecological distribution. Tumor and paracancerous tissues were more microbially diverse than other oral niches. Cancer surface, contralateral normal mucosa, saliva, and tongue coat showed similar microbial compositions, especially the contralateral normal mucosa and saliva. Periodontitis-associated bacteria of the genera Fusobacterium, Prevotella, Porphyromonas, Campylobacter, and Aggregatibacter, and anaerobic bacteria were enriched in tumor samples. The microbiome was highly correlated with tumor clinicopathological features, with several genera (Lautropia, Asteroleplasma, Parvimonas, Peptostreptococcus, Pyramidobacter, Roseburia, and Propionibacterium) demonstrating a relatively high diagnostic power for OSCC metastasis, potentially providing an indicator for the development of OSCC. |
| first_indexed | 2024-12-10T19:46:17Z |
| format | Article |
| id | doaj.art-107964a0a6e94d66ae6a20a1b38ceb18 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T19:46:17Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-107964a0a6e94d66ae6a20a1b38ceb182022-12-22T01:35:52ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-08-011210.3389/fcimb.2022.905653905653Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell CarcinomaFujiao Nie0Fujiao Nie1Lihua Wang2Lihua Wang3Yingying Huang4Yingying Huang5Pishan Yang6Pishan Yang7Pizhang Gong8Pizhang Gong9Qiang Feng10Qiang Feng11Chengzhe Yang12Chengzhe Yang13Department of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, ChinaShandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, ChinaDepartment of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Human Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, ChinaInstitute of Stomatology, Shandong University, Jinan, ChinaDepartment of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, ChinaShandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, ChinaDepartment of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, ChinaShandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, ChinaDepartment of Periodontology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Human Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, ChinaDepartment of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, ChinaInstitute of Stomatology, Shandong University, Jinan, ChinaOral squamous cell carcinoma (OSCC), one of the most common malignant tumors of the head and neck, is closely associated with the presence of oral microbes. However, the microbiomes of different oral niches in OSCC patients and their association with OSCC have not been adequately characterized. In this study, 305 samples were collected from 65 OSCC patients, including tumor tissue, adjacent normal tissue (paracancerous tissue), cancer surface tissue, anatomically matched contralateral normal mucosa, saliva, and tongue coat. 16S ribosomal DNA (16S rDNA) sequencing was used to compare the microbial composition, distribution, and co-occurrence network of different oral niches. The association between the microbiome and the clinical features of OSCC was also characterized. The oral microbiome of OSCC patients showed a regular ecological distribution. Tumor and paracancerous tissues were more microbially diverse than other oral niches. Cancer surface, contralateral normal mucosa, saliva, and tongue coat showed similar microbial compositions, especially the contralateral normal mucosa and saliva. Periodontitis-associated bacteria of the genera Fusobacterium, Prevotella, Porphyromonas, Campylobacter, and Aggregatibacter, and anaerobic bacteria were enriched in tumor samples. The microbiome was highly correlated with tumor clinicopathological features, with several genera (Lautropia, Asteroleplasma, Parvimonas, Peptostreptococcus, Pyramidobacter, Roseburia, and Propionibacterium) demonstrating a relatively high diagnostic power for OSCC metastasis, potentially providing an indicator for the development of OSCC.https://www.frontiersin.org/articles/10.3389/fcimb.2022.905653/fulloral squamous cell carcinomaoral microbiomedistribution characteristicsoral nichesclinicopathological features |
| spellingShingle | Fujiao Nie Fujiao Nie Lihua Wang Lihua Wang Yingying Huang Yingying Huang Pishan Yang Pishan Yang Pizhang Gong Pizhang Gong Qiang Feng Qiang Feng Chengzhe Yang Chengzhe Yang Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma Frontiers in Cellular and Infection Microbiology oral squamous cell carcinoma oral microbiome distribution characteristics oral niches clinicopathological features |
| title | Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma |
| title_full | Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma |
| title_fullStr | Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma |
| title_short | Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma |
| title_sort | characteristics of microbial distribution in different oral niches of oral squamous cell carcinoma |
| topic | oral squamous cell carcinoma oral microbiome distribution characteristics oral niches clinicopathological features |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.905653/full |
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