Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA System
The purpose of this study was to compare four activation solutions (AS)—Woynarovich, Lahnsteiner, Kucharczyk, and Perchec—with the addition of 0.5% bovine serum albumin (BSA) for ide (<i>Leuciscus idus</i>) sperm activation and analysis with a CASA system. It was found that ide sperm can...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/4/572 |
| _version_ | 1797622817573830656 |
|---|---|
| author | Beata Irena Cejko Sławomir Krejszeff Agata Anna Cejko Katarzyna Dryl |
| author_facet | Beata Irena Cejko Sławomir Krejszeff Agata Anna Cejko Katarzyna Dryl |
| author_sort | Beata Irena Cejko |
| collection | DOAJ |
| description | The purpose of this study was to compare four activation solutions (AS)—Woynarovich, Lahnsteiner, Kucharczyk, and Perchec—with the addition of 0.5% bovine serum albumin (BSA) for ide (<i>Leuciscus idus</i>) sperm activation and analysis with a CASA system. It was found that ide sperm can be activated using each AS within a pH range of 7.4–9.0 and an osmolality range of 160–200 mOsm kg<sup>−1</sup>. The effect of Woynarovich and Perchec solutions supplemented with BSA and casein at concentrations of 0.25, 0.5, 1.0, and 2.0% were also analyzed during the experiment. These two AS without protein supplementation (pure solutions) were the controls. Woynarovich and Perchec solutions supplemented with the minimum BSA concentration (i.e., 0.25%) significantly improved sperm motility (89.05% and 86.63%, respectively) compared to the controls (20.39 and 28.48%, respectively). Similar increases were also noted in progressively motile sperm (PRG, %), the curvilinear velocity of sperm (VCL, µm s<sup>−1</sup>), and the amplitude of lateral head displacement (ALH, µm). A similar trend in CASA parameters was also noted when casein was added to Woynarovich and Perchec solutions at a concentration of 0.25%. We concluded that 0.25% doses of each of the proteins were sufficient to prevent sperm adhesion to glass slides, and they can be used in research on ide sperm motility measurements. |
| first_indexed | 2024-03-11T09:15:38Z |
| format | Article |
| id | doaj.art-1079c731ba58465b8e510fa9cdb16db3 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:15:38Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1079c731ba58465b8e510fa9cdb16db32023-11-16T18:38:27ZengMDPI AGAnimals2076-26152023-02-0113457210.3390/ani13040572Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA SystemBeata Irena Cejko0Sławomir Krejszeff1Agata Anna Cejko2Katarzyna Dryl3Department of Salmonid Research, Stanisław Sakowicz Inland Fisheries Institute, 10-719 Olsztyn, PolandDepartment of Aquaculture, Stanisław Sakowicz Inland Fisheries Institute, 10-917 Olsztyn, PolandDepartment of Fisheries Bioeconomics, Stanisław Sakowicz Inland Fisheries Institute, 10-719 Olsztyn, PolandDepartment of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-243 Olsztyn, PolandThe purpose of this study was to compare four activation solutions (AS)—Woynarovich, Lahnsteiner, Kucharczyk, and Perchec—with the addition of 0.5% bovine serum albumin (BSA) for ide (<i>Leuciscus idus</i>) sperm activation and analysis with a CASA system. It was found that ide sperm can be activated using each AS within a pH range of 7.4–9.0 and an osmolality range of 160–200 mOsm kg<sup>−1</sup>. The effect of Woynarovich and Perchec solutions supplemented with BSA and casein at concentrations of 0.25, 0.5, 1.0, and 2.0% were also analyzed during the experiment. These two AS without protein supplementation (pure solutions) were the controls. Woynarovich and Perchec solutions supplemented with the minimum BSA concentration (i.e., 0.25%) significantly improved sperm motility (89.05% and 86.63%, respectively) compared to the controls (20.39 and 28.48%, respectively). Similar increases were also noted in progressively motile sperm (PRG, %), the curvilinear velocity of sperm (VCL, µm s<sup>−1</sup>), and the amplitude of lateral head displacement (ALH, µm). A similar trend in CASA parameters was also noted when casein was added to Woynarovich and Perchec solutions at a concentration of 0.25%. We concluded that 0.25% doses of each of the proteins were sufficient to prevent sperm adhesion to glass slides, and they can be used in research on ide sperm motility measurements.https://www.mdpi.com/2076-2615/13/4/572idesperm motilitysperm adhesionalbumincaseinCASA |
| spellingShingle | Beata Irena Cejko Sławomir Krejszeff Agata Anna Cejko Katarzyna Dryl Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA System Animals ide sperm motility sperm adhesion albumin casein CASA |
| title | Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA System |
| title_full | Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA System |
| title_fullStr | Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA System |
| title_full_unstemmed | Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA System |
| title_short | Effect of Different Activation Solutions and Protein Concentrations on Ide (<i>Leuciscus idus</i>) Sperm Motility Analysis with a CASA System |
| title_sort | effect of different activation solutions and protein concentrations on ide i leuciscus idus i sperm motility analysis with a casa system |
| topic | ide sperm motility sperm adhesion albumin casein CASA |
| url | https://www.mdpi.com/2076-2615/13/4/572 |
| work_keys_str_mv | AT beatairenacejko effectofdifferentactivationsolutionsandproteinconcentrationsonideileuciscusidusispermmotilityanalysiswithacasasystem AT sławomirkrejszeff effectofdifferentactivationsolutionsandproteinconcentrationsonideileuciscusidusispermmotilityanalysiswithacasasystem AT agataannacejko effectofdifferentactivationsolutionsandproteinconcentrationsonideileuciscusidusispermmotilityanalysiswithacasasystem AT katarzynadryl effectofdifferentactivationsolutionsandproteinconcentrationsonideileuciscusidusispermmotilityanalysiswithacasasystem |