Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model
Objective To investigate the effect and mechanism of pterostilbene (Pte) on paraquat (PQ)-induced pulmonary fibrosis in rats. Methods The rats were randomly divided into control group, PQ group (intragastric administration of 50 mg/kg paraquat at one time), and low, medium and high dose of Pte inter...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-09-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-9-1383.pdf |
| _version_ | 1797366246251954176 |
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| author | CHEN Xinjun, WANG Qinyu, CHEN Le, GU Chunyu, WU Zhuo |
| author_facet | CHEN Xinjun, WANG Qinyu, CHEN Le, GU Chunyu, WU Zhuo |
| author_sort | CHEN Xinjun, WANG Qinyu, CHEN Le, GU Chunyu, WU Zhuo |
| collection | DOAJ |
| description | Objective To investigate the effect and mechanism of pterostilbene (Pte) on paraquat (PQ)-induced pulmonary fibrosis in rats. Methods The rats were randomly divided into control group, PQ group (intragastric administration of 50 mg/kg paraquat at one time), and low, medium and high dose of Pte intervention group (after 30 minutes of intragastric administration of PQ, 25, 50 and 100 mg/kg Pte were administered respectively. Once a day for 7 days). After 7 days of treatment, the morphology of lung tissue was evaluated by hematoxylin-eosin (HE) staining microscopy and pulmonary fibrosis was evaluated by Masson trichrome staining. The level of IL-1β, IL-6 and MIP-2 in bronchoalveolar lavage fluid (BALF) was determined by ELISA. MDA and SOD contents in lung tissues were detected with commercially available kit. The protein expression of E-cadherin, α-SMA, vimentin,Nrf2, NF-κB p65, NF-κB p65, TGF-β1, Smad3 and p-Smad3 in lung tissue was detected by Western blot. Results Compared with the control group, the injury degree of lung tissue, the score of pulmonary fibrosis, the level of IL-1β, IL-6 and MIP-2 in BALF, the content of MDA in lung tissue, the protein expression of α-SMA, vimentin and TGF-β1 and the phosphorylation level of NF-κB p65 and Smad3 protein in lung tissue were all significantly increased in PQ group (P＜0.05), while the content of SOD and the protein expression of E-cadherin and Nrf2 in lung tissue were significantly decreased (P＜0.05). After Pte intervention, the injury of lung tissue, the score of pulmonary fibrosis, the level of IL-1β, IL-6 and MIP-2 in BALF, the content of MDA in lung tissue, the protein expression of α-SMA, vimentin and TGF-β1 and the phosphorylation of NF-κB p65 and Smad3 protein in lung tissue were all significantly decreased in Pte group (P＜0.05), while the content of SOD and the protein expression of E-cadherin and Nrf2 in lung tissue were significantly increased (P＜0.05). Conclusions Pterostilbene may inhibit fibrosis progression by reducing oxidative stress and decrease of inflammatory response in the lung through activation of Nrf2 and inhibition of NF-κB and TGF-β1/Smad2/3 signaling pathways. |
| first_indexed | 2024-03-08T17:02:37Z |
| format | Article |
| id | doaj.art-107bd0d905d8437d94833363feae4d05 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:02:37Z |
| publishDate | 2023-09-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-107bd0d905d8437d94833363feae4d052024-01-04T07:33:35ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-09-014391383138910.16352/j.issn.1001-6325.2023.09.1383Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat modelCHEN Xinjun, WANG Qinyu, CHEN Le, GU Chunyu, WU Zhuo01. Department of Emergency Internal Medicine; 2. Department of Medical Equipment, Shaanxi Provincial People's Hospital, Xi'an 710068, ChinaObjective To investigate the effect and mechanism of pterostilbene (Pte) on paraquat (PQ)-induced pulmonary fibrosis in rats. Methods The rats were randomly divided into control group, PQ group (intragastric administration of 50 mg/kg paraquat at one time), and low, medium and high dose of Pte intervention group (after 30 minutes of intragastric administration of PQ, 25, 50 and 100 mg/kg Pte were administered respectively. Once a day for 7 days). After 7 days of treatment, the morphology of lung tissue was evaluated by hematoxylin-eosin (HE) staining microscopy and pulmonary fibrosis was evaluated by Masson trichrome staining. The level of IL-1β, IL-6 and MIP-2 in bronchoalveolar lavage fluid (BALF) was determined by ELISA. MDA and SOD contents in lung tissues were detected with commercially available kit. The protein expression of E-cadherin, α-SMA, vimentin,Nrf2, NF-κB p65, NF-κB p65, TGF-β1, Smad3 and p-Smad3 in lung tissue was detected by Western blot. Results Compared with the control group, the injury degree of lung tissue, the score of pulmonary fibrosis, the level of IL-1β, IL-6 and MIP-2 in BALF, the content of MDA in lung tissue, the protein expression of α-SMA, vimentin and TGF-β1 and the phosphorylation level of NF-κB p65 and Smad3 protein in lung tissue were all significantly increased in PQ group (P＜0.05), while the content of SOD and the protein expression of E-cadherin and Nrf2 in lung tissue were significantly decreased (P＜0.05). After Pte intervention, the injury of lung tissue, the score of pulmonary fibrosis, the level of IL-1β, IL-6 and MIP-2 in BALF, the content of MDA in lung tissue, the protein expression of α-SMA, vimentin and TGF-β1 and the phosphorylation of NF-κB p65 and Smad3 protein in lung tissue were all significantly decreased in Pte group (P＜0.05), while the content of SOD and the protein expression of E-cadherin and Nrf2 in lung tissue were significantly increased (P＜0.05). Conclusions Pterostilbene may inhibit fibrosis progression by reducing oxidative stress and decrease of inflammatory response in the lung through activation of Nrf2 and inhibition of NF-κB and TGF-β1/Smad2/3 signaling pathways.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-9-1383.pdfpterostilbene|paraquat|pulmonary fibrosis|oxidative stress|inflammation |
| spellingShingle | CHEN Xinjun, WANG Qinyu, CHEN Le, GU Chunyu, WU Zhuo Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model Jichu yixue yu linchuang pterostilbene|paraquat|pulmonary fibrosis|oxidative stress|inflammation |
| title | Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model |
| title_full | Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model |
| title_fullStr | Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model |
| title_full_unstemmed | Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model |
| title_short | Pterostilbene reduces paraquat-induced pulmonary fibrosis in rat model |
| title_sort | pterostilbene reduces paraquat induced pulmonary fibrosis in rat model |
| topic | pterostilbene|paraquat|pulmonary fibrosis|oxidative stress|inflammation |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-9-1383.pdf |
| work_keys_str_mv | AT chenxinjunwangqinyuchenleguchunyuwuzhuo pterostilbenereducesparaquatinducedpulmonaryfibrosisinratmodel |