Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells
Background: Although the influence of titanium implants’ micro-surface properties on titanium discs has been extensively investigated, the research has not taken into consideration their whole-body effect, which may be considered possible using a combinatorial approach. Methods: Five titanium dental...
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| Format: | Članak |
| Jezik: | English |
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MDPI AG
2022-10-01
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| Serija: | Journal of Functional Biomaterials |
| Teme: | |
| Online pristup: | https://www.mdpi.com/2079-4983/13/4/206 |
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| author | Federico Ferro Federico Azzolin Renza Spelat Lorenzo Bevilacqua Michele Maglione |
| author_facet | Federico Ferro Federico Azzolin Renza Spelat Lorenzo Bevilacqua Michele Maglione |
| author_sort | Federico Ferro |
| collection | DOAJ |
| description | Background: Although the influence of titanium implants’ micro-surface properties on titanium discs has been extensively investigated, the research has not taken into consideration their whole-body effect, which may be considered possible using a combinatorial approach. Methods: Five titanium dental implants with a similar moderate roughness and different surface textures were thoroughly characterized. The cell adhesion and proliferation were assessed after adipose-tissue-derived stem cells (ADSCs) were seeded on whole-body implants. The implants’ inductive properties were assessed by evaluating the osteoblastic gene expression. Results: The surface micro-topography was analyzed, showing that hydroxyapatite (HA)-blasted and bland acid etching implants had the highest roughness and a lower number of surface particles. Cell adhesion was observed after 24 h on all the implants, with the highest score registered for the HA-blasted and bland acid etching implants. Cell proliferation was observed only on the laser-treated and double-acid-etched surfaces. The ADSCs expressed collagen type I, osteonectin, and alkaline phosphatase on all the implant surfaces, with high levels on the HA-treated surfaces, which also triggered osteocalcin expression on day seven. Conclusions: The findings of this study show that the morphology and treatment of whole titanium dental implants, primarily HA-treated and bland acid etching implants, impact the adherence and activity of ADSCs in osteogenic differentiation in the absence of specific osteo-inductive signals. |
| first_indexed | 2024-03-09T16:15:30Z |
| format | Article |
| id | doaj.art-107c0095b042452aa1cdf733b5aa095c |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T16:15:30Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-107c0095b042452aa1cdf733b5aa095c2023-11-24T15:49:27ZengMDPI AGJournal of Functional Biomaterials2079-49832022-10-0113420610.3390/jfb13040206Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem CellsFederico Ferro0Federico Azzolin1Renza Spelat2Lorenzo Bevilacqua3Michele Maglione4Department of Medical and Biological Sciences, University of Udine, 33100 Udine, ItalyDepartment of Medical, Surgery and Health Sciences, University of Trieste, 34125 Trieste, ItalyNeurobiology Sector, International School for Advanced Studies (SISSA), 34136 Trieste, ItalyDepartment of Medical, Surgery and Health Sciences, University of Trieste, 34125 Trieste, ItalyDepartment of Medical, Surgery and Health Sciences, University of Trieste, 34125 Trieste, ItalyBackground: Although the influence of titanium implants’ micro-surface properties on titanium discs has been extensively investigated, the research has not taken into consideration their whole-body effect, which may be considered possible using a combinatorial approach. Methods: Five titanium dental implants with a similar moderate roughness and different surface textures were thoroughly characterized. The cell adhesion and proliferation were assessed after adipose-tissue-derived stem cells (ADSCs) were seeded on whole-body implants. The implants’ inductive properties were assessed by evaluating the osteoblastic gene expression. Results: The surface micro-topography was analyzed, showing that hydroxyapatite (HA)-blasted and bland acid etching implants had the highest roughness and a lower number of surface particles. Cell adhesion was observed after 24 h on all the implants, with the highest score registered for the HA-blasted and bland acid etching implants. Cell proliferation was observed only on the laser-treated and double-acid-etched surfaces. The ADSCs expressed collagen type I, osteonectin, and alkaline phosphatase on all the implant surfaces, with high levels on the HA-treated surfaces, which also triggered osteocalcin expression on day seven. Conclusions: The findings of this study show that the morphology and treatment of whole titanium dental implants, primarily HA-treated and bland acid etching implants, impact the adherence and activity of ADSCs in osteogenic differentiation in the absence of specific osteo-inductive signals.https://www.mdpi.com/2079-4983/13/4/206periodontologysurface chemistrybone implant interactionscell–implant interactions |
| spellingShingle | Federico Ferro Federico Azzolin Renza Spelat Lorenzo Bevilacqua Michele Maglione Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells Journal of Functional Biomaterials periodontology surface chemistry bone implant interactions cell–implant interactions |
| title | Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells |
| title_full | Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells |
| title_fullStr | Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells |
| title_full_unstemmed | Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells |
| title_short | Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells |
| title_sort | assessing the efficacy of whole body titanium dental implant surface modifications in inducing adhesion proliferation and osteogenesis in human adipose tissue stem cells |
| topic | periodontology surface chemistry bone implant interactions cell–implant interactions |
| url | https://www.mdpi.com/2079-4983/13/4/206 |
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