Protocol to analyze antitumor immunity of orthotopic injection and spontaneous murine high-grade glioma models using flow cytometry and single-cell RNA sequencing

Protocol to analyze antitumor immunity of orthotopic injection and spontaneous murine high-grade glioma models using flow cytometry and single-cell RNA sequencing

Summary: Despite the recognized importance of antitumor immunity, our understanding of brain tumor immunity is poor. Orthotopic injection models have been widely used for immunological analyses. However, these models have limitations in analysis of antitumor immunity because the approach involves dr...

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Bibliographic Details
Main Authors: Jang Hyun Park, Chae Won Kim, Hyun-Jin Kim, Hyun Jung Kim, Jeong Ho Lee, Heung Kyu Lee
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell isolation
Single cell
Flow cytometry/Mass cytometry
Cancer
Sequencing
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004877

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