Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic Farming
In this study, broccoli grown with deep sea water minerals (DSWMs) confirmed anticancer effects in HT-29 colorectal cancer cells and anti-inflammatory effects in C57BL/6 mouse splenocytes. Natural dream cultured broccoli (NB) grown with DSWMs had elevated sodium (Na) and calcium (Ca) levels and enha...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/17/9684 |
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| author | Yeon-Jun Lee Yanni Pan Seung-Hwan Park Sin-Il Sin Kun-Young Park |
| author_facet | Yeon-Jun Lee Yanni Pan Seung-Hwan Park Sin-Il Sin Kun-Young Park |
| author_sort | Yeon-Jun Lee |
| collection | DOAJ |
| description | In this study, broccoli grown with deep sea water minerals (DSWMs) confirmed anticancer effects in HT-29 colorectal cancer cells and anti-inflammatory effects in C57BL/6 mouse splenocytes. Natural dream cultured broccoli (NB) grown with DSWMs had elevated sodium (Na) and calcium (Ca) levels and enhanced the expression of p53 and p21, both of which are associated with cell cycle arrest in HT-29 colorectal cancer cells. It also decreased the expression of Bax, Bad, Bim, Bak, caspase-9, and caspase-3. In lipopolysaccharide (LPS)-treated C57BL/6 mouse splenocytes, NB produced little nitric oxide (NO). ELISA research indicated that NB decreased IL-1β, IL-6, TNF-α, IFN-γ, and IL-12 expression while increasing NK cell activity. As a result, broccoli cultivated with deep water minerals has better anticancer and anti-inflammatory properties than conventional and organic farming. |
| first_indexed | 2024-03-10T23:27:21Z |
| format | Article |
| id | doaj.art-107dc37a93e2452fb8ff2b45779a8cb0 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T23:27:21Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-107dc37a93e2452fb8ff2b45779a8cb02023-11-19T07:50:23ZengMDPI AGApplied Sciences2076-34172023-08-011317968410.3390/app13179684Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic FarmingYeon-Jun Lee0Yanni Pan1Seung-Hwan Park2Sin-Il Sin3Kun-Young Park4Department of Food Science and Biotechnology, CHA University, Seongnam 13488, Republic of KoreaDepartment of Food Science and Biotechnology, CHA University, Seongnam 13488, Republic of KoreaAgriculture Research Center for Carbon Neutral and Healing, Gurye-gun 57607, Republic of KoreaAgriculture Research Center for Carbon Neutral and Healing, Gurye-gun 57607, Republic of KoreaCollaborative Innovation Center for Child Nutrition and Health Development, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, ChinaIn this study, broccoli grown with deep sea water minerals (DSWMs) confirmed anticancer effects in HT-29 colorectal cancer cells and anti-inflammatory effects in C57BL/6 mouse splenocytes. Natural dream cultured broccoli (NB) grown with DSWMs had elevated sodium (Na) and calcium (Ca) levels and enhanced the expression of p53 and p21, both of which are associated with cell cycle arrest in HT-29 colorectal cancer cells. It also decreased the expression of Bax, Bad, Bim, Bak, caspase-9, and caspase-3. In lipopolysaccharide (LPS)-treated C57BL/6 mouse splenocytes, NB produced little nitric oxide (NO). ELISA research indicated that NB decreased IL-1β, IL-6, TNF-α, IFN-γ, and IL-12 expression while increasing NK cell activity. As a result, broccoli cultivated with deep water minerals has better anticancer and anti-inflammatory properties than conventional and organic farming.https://www.mdpi.com/2076-3417/13/17/9684anti-inflammationanticancerbroccolicultivationdeep sea water minerals |
| spellingShingle | Yeon-Jun Lee Yanni Pan Seung-Hwan Park Sin-Il Sin Kun-Young Park Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic Farming Applied Sciences anti-inflammation anticancer broccoli cultivation deep sea water minerals |
| title | Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic Farming |
| title_full | Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic Farming |
| title_fullStr | Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic Farming |
| title_full_unstemmed | Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic Farming |
| title_short | Anticancer Effects in HT-29 Cells and Anti-Inflammatory Effects on Mouse Splenocytes of Broccoli Cultivated with Deep Sea Water Minerals in Organic Farming |
| title_sort | anticancer effects in ht 29 cells and anti inflammatory effects on mouse splenocytes of broccoli cultivated with deep sea water minerals in organic farming |
| topic | anti-inflammation anticancer broccoli cultivation deep sea water minerals |
| url | https://www.mdpi.com/2076-3417/13/17/9684 |
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