Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes
Astroglial cells isolated from the rodent postnatal cerebral cortex are particularly susceptible to lineage reprogramming into neurons. However, it remains unknown whether other astroglial populations retain the same potential. Likewise, little is known about the fate of induced neurons (iNs) in viv...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117302163 |
| _version_ | 1811192427549032448 |
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| author | Malek Chouchane Ana Raquel Melo de Farias Daniela Maria de Sousa Moura Markus Michael Hilscher Timm Schroeder Richardson Naves Leão Marcos Romualdo Costa |
| author_facet | Malek Chouchane Ana Raquel Melo de Farias Daniela Maria de Sousa Moura Markus Michael Hilscher Timm Schroeder Richardson Naves Leão Marcos Romualdo Costa |
| author_sort | Malek Chouchane |
| collection | DOAJ |
| description | Astroglial cells isolated from the rodent postnatal cerebral cortex are particularly susceptible to lineage reprogramming into neurons. However, it remains unknown whether other astroglial populations retain the same potential. Likewise, little is known about the fate of induced neurons (iNs) in vivo. In this study we addressed these questions using two different astroglial populations isolated from the postnatal brain reprogrammed either with Neurogenin-2 (Neurog2) or Achaete scute homolog-1 (Ascl1). We show that cerebellum (CerebAstro) and cerebral cortex astroglia (CtxAstro) generates iNs with distinctive neurochemical and morphological properties. Both astroglial populations contribute iNs to the olfactory bulb following transplantation in the postnatal and adult mouse subventricular zone. However, only CtxAstro transfected with Neurog2 differentiate into pyramidal-like iNs after transplantation in the postnatal cerebral cortex. Altogether, our data indicate that the origin of the astroglial population and transcription factors used for reprogramming, as well as the region of integration, affect the fate of iNs. |
| first_indexed | 2024-04-11T23:53:40Z |
| format | Article |
| id | doaj.art-10821dc38c664730af3aa2a4282c10ea |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-11T23:53:40Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-10821dc38c664730af3aa2a4282c10ea2022-12-22T03:56:25ZengElsevierStem Cell Reports2213-67112017-07-019116217610.1016/j.stemcr.2017.05.009Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal SubtypesMalek Chouchane0Ana Raquel Melo de Farias1Daniela Maria de Sousa Moura2Markus Michael Hilscher3Timm Schroeder4Richardson Naves Leão5Marcos Romualdo Costa6Brain Institute, Federal University of Rio Grande do Norte, Natal 59056-450, BrazilBrain Institute, Federal University of Rio Grande do Norte, Natal 59056-450, BrazilBrain Institute, Federal University of Rio Grande do Norte, Natal 59056-450, BrazilBrain Institute, Federal University of Rio Grande do Norte, Natal 59056-450, BrazilDepartment of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel, SwitzerlandBrain Institute, Federal University of Rio Grande do Norte, Natal 59056-450, BrazilBrain Institute, Federal University of Rio Grande do Norte, Natal 59056-450, BrazilAstroglial cells isolated from the rodent postnatal cerebral cortex are particularly susceptible to lineage reprogramming into neurons. However, it remains unknown whether other astroglial populations retain the same potential. Likewise, little is known about the fate of induced neurons (iNs) in vivo. In this study we addressed these questions using two different astroglial populations isolated from the postnatal brain reprogrammed either with Neurogenin-2 (Neurog2) or Achaete scute homolog-1 (Ascl1). We show that cerebellum (CerebAstro) and cerebral cortex astroglia (CtxAstro) generates iNs with distinctive neurochemical and morphological properties. Both astroglial populations contribute iNs to the olfactory bulb following transplantation in the postnatal and adult mouse subventricular zone. However, only CtxAstro transfected with Neurog2 differentiate into pyramidal-like iNs after transplantation in the postnatal cerebral cortex. Altogether, our data indicate that the origin of the astroglial population and transcription factors used for reprogramming, as well as the region of integration, affect the fate of iNs.http://www.sciencedirect.com/science/article/pii/S2213671117302163lineage reprogramminginduced neuronsproneural genesastroglial cellscerebral cortexcerebellumcell transplantation |
| spellingShingle | Malek Chouchane Ana Raquel Melo de Farias Daniela Maria de Sousa Moura Markus Michael Hilscher Timm Schroeder Richardson Naves Leão Marcos Romualdo Costa Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes Stem Cell Reports lineage reprogramming induced neurons proneural genes astroglial cells cerebral cortex cerebellum cell transplantation |
| title | Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes |
| title_full | Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes |
| title_fullStr | Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes |
| title_full_unstemmed | Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes |
| title_short | Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes |
| title_sort | lineage reprogramming of astroglial cells from different origins into distinct neuronal subtypes |
| topic | lineage reprogramming induced neurons proneural genes astroglial cells cerebral cortex cerebellum cell transplantation |
| url | http://www.sciencedirect.com/science/article/pii/S2213671117302163 |
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