First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producer
The dinoflagellate genus Prorocentrum includes several harmful toxigenic species, predominantly benthic ones. In the past, fast-acting toxicity in mice has been observed in extracts of the epibenthic species P. borbonicum, with the finding of two compounds termed as borbotoxins-A and -B. The presenc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.973250/full |
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| author | Edgar Arteaga-Sogamoso Edgar Arteaga-Sogamoso Pilar Riobó Francisco Rodríguez Francisco Rodríguez José Ernesto Mancera-Pineda Julián Franco-Angulo |
| author_facet | Edgar Arteaga-Sogamoso Edgar Arteaga-Sogamoso Pilar Riobó Francisco Rodríguez Francisco Rodríguez José Ernesto Mancera-Pineda Julián Franco-Angulo |
| author_sort | Edgar Arteaga-Sogamoso |
| collection | DOAJ |
| description | The dinoflagellate genus Prorocentrum includes several harmful toxigenic species, predominantly benthic ones. In the past, fast-acting toxicity in mice has been observed in extracts of the epibenthic species P. borbonicum, with the finding of two compounds termed as borbotoxins-A and -B. The presence of palytoxin-like compounds was also suggested from electrophysiological experiments. In the present study, a strain of P. borbonicum was isolated in the continental coast of Colombian Caribbean, in seagrass beds of Thalassia testudinum in Bonito Gordo, Tayrona National Natural Park (PNNT). The morphological and molecular characteristics were consistent with the original and former descriptions for this species. Typical haemolytic activity due to palytoxin was confirmed in P. borbonicum extracts in presence of ouabain, the toxin contents being estimated as 1.9 pg palytoxin equivalents cell-1. HPLC-HRMS analyses of these extracts unambiguously identified the presence of borbotoxins and 42-hidroxy-palytoxin (42-OH-PLTX). This is the first report of palytoxin-like compounds in another dinoflagellate genus than Ostreopsis and the first record of P. borbonicum in Colombia and the Caribbean region. |
| first_indexed | 2024-04-11T14:22:56Z |
| format | Article |
| id | doaj.art-10852e8908394122a66a011f49eedf0c |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-11T14:22:56Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-10852e8908394122a66a011f49eedf0c2022-12-22T04:19:00ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-08-01910.3389/fmars.2022.973250973250First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producerEdgar Arteaga-Sogamoso0Edgar Arteaga-Sogamoso1Pilar Riobó2Francisco Rodríguez3Francisco Rodríguez4José Ernesto Mancera-Pineda5Julián Franco-Angulo6Instituto de Investigaciones Marinas y Costeras José Benito Vives de Andréis, INVEMAR, Santa Marta, ColombiaUniversidad Nacional de Colombia, sede Caribe, Santa Marta, ColombiaInstituto de Investigaciones Marinas, CSIC, Vigo, SpainInstituto Español de Oceanografía (CNIEO-CSIC), Vigo, SpainEuropean Union Reference Laboratory for Monitoring of Marine Biotoxins, Citexvi Campus Universitario de Vigo, Vigo, SpainUniversidad Nacional de Colombia, sede Bogotá, ColombiaInstituto de Investigaciones Marinas y Costeras José Benito Vives de Andréis, INVEMAR, Santa Marta, ColombiaThe dinoflagellate genus Prorocentrum includes several harmful toxigenic species, predominantly benthic ones. In the past, fast-acting toxicity in mice has been observed in extracts of the epibenthic species P. borbonicum, with the finding of two compounds termed as borbotoxins-A and -B. The presence of palytoxin-like compounds was also suggested from electrophysiological experiments. In the present study, a strain of P. borbonicum was isolated in the continental coast of Colombian Caribbean, in seagrass beds of Thalassia testudinum in Bonito Gordo, Tayrona National Natural Park (PNNT). The morphological and molecular characteristics were consistent with the original and former descriptions for this species. Typical haemolytic activity due to palytoxin was confirmed in P. borbonicum extracts in presence of ouabain, the toxin contents being estimated as 1.9 pg palytoxin equivalents cell-1. HPLC-HRMS analyses of these extracts unambiguously identified the presence of borbotoxins and 42-hidroxy-palytoxin (42-OH-PLTX). This is the first report of palytoxin-like compounds in another dinoflagellate genus than Ostreopsis and the first record of P. borbonicum in Colombia and the Caribbean region.https://www.frontiersin.org/articles/10.3389/fmars.2022.973250/fullProrocentrumpalytoxinTayrona National Natural ParkColombian CaribbeanHPLC-HRMS |
| spellingShingle | Edgar Arteaga-Sogamoso Edgar Arteaga-Sogamoso Pilar Riobó Francisco Rodríguez Francisco Rodríguez José Ernesto Mancera-Pineda Julián Franco-Angulo First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producer Frontiers in Marine Science Prorocentrum palytoxin Tayrona National Natural Park Colombian Caribbean HPLC-HRMS |
| title | First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producer |
| title_full | First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producer |
| title_fullStr | First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producer |
| title_full_unstemmed | First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producer |
| title_short | First record of the dinoflagellate Prorocentrum borbonicum in the continental coast of Colombian Caribbean: A new 42 hydroxi-palytoxin producer |
| title_sort | first record of the dinoflagellate prorocentrum borbonicum in the continental coast of colombian caribbean a new 42 hydroxi palytoxin producer |
| topic | Prorocentrum palytoxin Tayrona National Natural Park Colombian Caribbean HPLC-HRMS |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.973250/full |
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