O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility.
Animal behavior is influenced by the competing drives to maintain energy and to reproduce. The balance between these evolutionary pressures and how nutrient signaling pathways intersect with mating remains unclear. The nutrient sensor O-GlcNAc transferase, which post-translationally modifies intrace...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-11-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010273 |
| _version_ | 1797973897450094592 |
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| author | Daniel Konzman Tetsunari Fukushige Mesgana Dagnachew Michael Krause John A Hanover |
| author_facet | Daniel Konzman Tetsunari Fukushige Mesgana Dagnachew Michael Krause John A Hanover |
| author_sort | Daniel Konzman |
| collection | DOAJ |
| description | Animal behavior is influenced by the competing drives to maintain energy and to reproduce. The balance between these evolutionary pressures and how nutrient signaling pathways intersect with mating remains unclear. The nutrient sensor O-GlcNAc transferase, which post-translationally modifies intracellular proteins with a single monosaccharide, is responsive to cellular nutrient status and regulates diverse biological processes. Though essential in most metazoans, O-GlcNAc transferase (ogt-1) is dispensable in Caenorhabditis elegans, allowing genetic analysis of its physiological roles. Compared to control, ogt-1 males had a four-fold reduction in mean offspring, with nearly two thirds producing zero progeny. Interestingly, we found that ogt-1 males transferred sperm less often, and virgin males had reduced sperm count. ogt-1 males were also less likely to engage in mate-searching and mate-response behaviors. Surprisingly, we found normal fertility for males with hypodermal expression of ogt-1 and for ogt-1 strains with catalytic-dead mutations. This suggests OGT-1 serves a non-catalytic function in the hypodermis impacting male fertility and mating behavior. This study builds upon research on the nutrient sensor O-GlcNAc transferase and demonstrates a role it plays in the interplay between the evolutionary drives for reproduction and survival. |
| first_indexed | 2024-04-11T04:11:29Z |
| format | Article |
| id | doaj.art-108872aff9ed4a81b357fcfc14a0b6f0 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-11T04:11:29Z |
| publishDate | 2022-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-108872aff9ed4a81b357fcfc14a0b6f02023-01-01T05:32:05ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-11-011811e101027310.1371/journal.pgen.1010273O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility.Daniel KonzmanTetsunari FukushigeMesgana DagnachewMichael KrauseJohn A HanoverAnimal behavior is influenced by the competing drives to maintain energy and to reproduce. The balance between these evolutionary pressures and how nutrient signaling pathways intersect with mating remains unclear. The nutrient sensor O-GlcNAc transferase, which post-translationally modifies intracellular proteins with a single monosaccharide, is responsive to cellular nutrient status and regulates diverse biological processes. Though essential in most metazoans, O-GlcNAc transferase (ogt-1) is dispensable in Caenorhabditis elegans, allowing genetic analysis of its physiological roles. Compared to control, ogt-1 males had a four-fold reduction in mean offspring, with nearly two thirds producing zero progeny. Interestingly, we found that ogt-1 males transferred sperm less often, and virgin males had reduced sperm count. ogt-1 males were also less likely to engage in mate-searching and mate-response behaviors. Surprisingly, we found normal fertility for males with hypodermal expression of ogt-1 and for ogt-1 strains with catalytic-dead mutations. This suggests OGT-1 serves a non-catalytic function in the hypodermis impacting male fertility and mating behavior. This study builds upon research on the nutrient sensor O-GlcNAc transferase and demonstrates a role it plays in the interplay between the evolutionary drives for reproduction and survival.https://doi.org/10.1371/journal.pgen.1010273 |
| spellingShingle | Daniel Konzman Tetsunari Fukushige Mesgana Dagnachew Michael Krause John A Hanover O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility. PLoS Genetics |
| title | O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility. |
| title_full | O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility. |
| title_fullStr | O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility. |
| title_full_unstemmed | O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility. |
| title_short | O-GlcNAc transferase plays a non-catalytic role in C. elegans male fertility. |
| title_sort | o glcnac transferase plays a non catalytic role in c elegans male fertility |
| url | https://doi.org/10.1371/journal.pgen.1010273 |
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