Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure
Phytate represents an organic pool of phosphorus in soil that requires hydrolysis by phytase enzymes produced by microorganisms prior to its bioavailability by plants. We tested the ability of a microbial suspension made from an old growth maple forest’s undisturbed soil to mineralize phytate in a g...
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MDPI AG
2021-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1803 |
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| author | Bulbul Ahmed Jean-Baptiste Floc’h Zakaria Lahrach Mohamed Hijri |
| author_facet | Bulbul Ahmed Jean-Baptiste Floc’h Zakaria Lahrach Mohamed Hijri |
| author_sort | Bulbul Ahmed |
| collection | DOAJ |
| description | Phytate represents an organic pool of phosphorus in soil that requires hydrolysis by phytase enzymes produced by microorganisms prior to its bioavailability by plants. We tested the ability of a microbial suspension made from an old growth maple forest’s undisturbed soil to mineralize phytate in a greenhouse trial on soybean plants inoculated or non-inoculated with the suspension. MiSeq Amplicon sequencing targeting bacterial 16S rRNA gene and fungal ITS was performed to assess microbial community changes following treatments. Our results showed that soybean nodulation and shoot dry weight biomass increased when phytate was applied to the nutrient-poor substrate mixture. Bacterial and fungal diversities of the root and rhizosphere biotopes were relatively resilient following inoculation by microbial suspension; however, bacterial community structure was significantly influenced. Interestingly, four arbuscular mycorrhizal fungi (AMF) were identified as indicator species, including <i>Glomus</i> sp., <i>Claroideoglomus etunicatum</i>, <i>Funneliformis mosseae</i> and an unidentified AMF taxon. We also observed that an ericoid mycorrhizal taxon <i>Sebacina</i> sp. and three <i>Trichoderma</i> spp. were among indicator species. Non-pathogenic Planctobacteria members highly dominated the bacterial community as core and hub taxa for over 80% of all bacterial datasets in root and rhizosphere biotopes. Overall, our study documented that inoculation with a microbial suspension and phytate amendment improved soybean plant growth. |
| first_indexed | 2024-03-10T07:25:43Z |
| format | Article |
| id | doaj.art-1089008c88b64381b69bd1fed1c38a4a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:43Z |
| publishDate | 2021-08-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1089008c88b64381b69bd1fed1c38a4a2023-11-22T14:17:18ZengMDPI AGMicroorganisms2076-26072021-08-0199180310.3390/microorganisms9091803Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community StructureBulbul Ahmed0Jean-Baptiste Floc’h1Zakaria Lahrach2Mohamed Hijri3Institut de Recherche en Biologie Végétale, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, CanadaInstitut de Recherche en Biologie Végétale, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, CanadaInstitut de Recherche en Biologie Végétale, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, CanadaInstitut de Recherche en Biologie Végétale, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, CanadaPhytate represents an organic pool of phosphorus in soil that requires hydrolysis by phytase enzymes produced by microorganisms prior to its bioavailability by plants. We tested the ability of a microbial suspension made from an old growth maple forest’s undisturbed soil to mineralize phytate in a greenhouse trial on soybean plants inoculated or non-inoculated with the suspension. MiSeq Amplicon sequencing targeting bacterial 16S rRNA gene and fungal ITS was performed to assess microbial community changes following treatments. Our results showed that soybean nodulation and shoot dry weight biomass increased when phytate was applied to the nutrient-poor substrate mixture. Bacterial and fungal diversities of the root and rhizosphere biotopes were relatively resilient following inoculation by microbial suspension; however, bacterial community structure was significantly influenced. Interestingly, four arbuscular mycorrhizal fungi (AMF) were identified as indicator species, including <i>Glomus</i> sp., <i>Claroideoglomus etunicatum</i>, <i>Funneliformis mosseae</i> and an unidentified AMF taxon. We also observed that an ericoid mycorrhizal taxon <i>Sebacina</i> sp. and three <i>Trichoderma</i> spp. were among indicator species. Non-pathogenic Planctobacteria members highly dominated the bacterial community as core and hub taxa for over 80% of all bacterial datasets in root and rhizosphere biotopes. Overall, our study documented that inoculation with a microbial suspension and phytate amendment improved soybean plant growth.https://www.mdpi.com/2076-2607/9/9/1803microbiomephytatesoybeanphosphorusnetworkMiSeq |
| spellingShingle | Bulbul Ahmed Jean-Baptiste Floc’h Zakaria Lahrach Mohamed Hijri Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure Microorganisms microbiome phytate soybean phosphorus network MiSeq |
| title | Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure |
| title_full | Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure |
| title_fullStr | Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure |
| title_full_unstemmed | Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure |
| title_short | Phytate and Microbial Suspension Amendments Increased Soybean Growth and Shifted Microbial Community Structure |
| title_sort | phytate and microbial suspension amendments increased soybean growth and shifted microbial community structure |
| topic | microbiome phytate soybean phosphorus network MiSeq |
| url | https://www.mdpi.com/2076-2607/9/9/1803 |
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