Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons
Abstract Adult tendons are highly differentiated. In mature individuals, tendon healing after an injury occurs through fibrotic tissue formation. Understanding the intrinsic reparative properties of fetal tendons would help to understand the maturation tissue process and tendon tissue repair. The pr...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19695-3 |
| _version_ | 1811192367810609152 |
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| author | Rodrigo Sousa Macedo Walcy Rosolia Teodoro Vera Luiza Capellozzi Dov Lagus Rosemberg Rafael Barban Sposeto Cesar de Cesar Netto Jonathan T. Deland Nicola Maffulli Scott J. Ellis Alexandre Leme Godoy-Santos |
| author_facet | Rodrigo Sousa Macedo Walcy Rosolia Teodoro Vera Luiza Capellozzi Dov Lagus Rosemberg Rafael Barban Sposeto Cesar de Cesar Netto Jonathan T. Deland Nicola Maffulli Scott J. Ellis Alexandre Leme Godoy-Santos |
| author_sort | Rodrigo Sousa Macedo |
| collection | DOAJ |
| description | Abstract Adult tendons are highly differentiated. In mature individuals, tendon healing after an injury occurs through fibrotic tissue formation. Understanding the intrinsic reparative properties of fetal tendons would help to understand the maturation tissue process and tendon tissue repair. The present study evaluated the evolution of histoarchitecture, cellularity and the distribution of collagens I, III and V in the posterior tibial tendon in human fetuses at different gestational ages. Morphological profiles were assessed in nine fresh spontaneously aborted fetuses (Group I: five fetuses aged between 22 and 28 weeks of gestation; Group II: four fetuses aged between 32 and 38 weeks of gestation), characterized by a combination of histology, fluorescence and immunohistochemistry. In Group I, the posterior tibial tendon showed statistically significant greater cellularity and presence of collagen III and V than in Group II tendon, which showed a predominance of collagenous I and a better organization of the extracellular matrix compared with Group I tendons. In addition, a statistically significant higher rate of CD90, a marker of mesenchymal cells, was found in Group I tendons. In fetuses with gestational age between 22 and 28 weeks, the posterior tibialis tendons showed a thin and disorganized fibrillar structure, with an increase in collagen III and V fibers and mesenchymal cells. In the posterior tibialis tendons of fetuses with gestational age between 32 and 38 weeks, the fibrillar structure was thicker with a statistically significant increase in type I collagen and decreased cellularity. |
| first_indexed | 2024-04-11T23:52:35Z |
| format | Article |
| id | doaj.art-108a94265237441faba73de8124510eb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T23:52:35Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-108a94265237441faba73de8124510eb2022-12-22T03:56:27ZengNature PortfolioScientific Reports2045-23222022-10-011211810.1038/s41598-022-19695-3Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendonsRodrigo Sousa Macedo0Walcy Rosolia Teodoro1Vera Luiza Capellozzi2Dov Lagus Rosemberg3Rafael Barban Sposeto4Cesar de Cesar Netto5Jonathan T. Deland6Nicola Maffulli7Scott J. Ellis8Alexandre Leme Godoy-Santos9Lab. Prof. Manlio Mario Marco Napoli, Departamento de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de São PauloRheumatology Division of the Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, FMUSPDepartment of Pathology of the Hospital das Clinicas, Faculdade de Medicina, Universidade de São Paulo, FMUSPLab. Prof. Manlio Mario Marco Napoli, Departamento de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de São PauloLab. Prof. Manlio Mario Marco Napoli, Departamento de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de São PauloFoot and Ankle Services, Department of Orthopedics and Rehabilitation, University of Iowa, Carver College of Medicine, John Pappajohn Pavilion (JPP)Department of Foot and Ankle Surgery, Hospital for Special SurgeryDepartment of Medicine, Surgery and Dentistry, University of SalernoDepartment of Foot and Ankle Surgery, Hospital for Special SurgeryLab. Prof. Manlio Mario Marco Napoli, Departamento de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de São PauloAbstract Adult tendons are highly differentiated. In mature individuals, tendon healing after an injury occurs through fibrotic tissue formation. Understanding the intrinsic reparative properties of fetal tendons would help to understand the maturation tissue process and tendon tissue repair. The present study evaluated the evolution of histoarchitecture, cellularity and the distribution of collagens I, III and V in the posterior tibial tendon in human fetuses at different gestational ages. Morphological profiles were assessed in nine fresh spontaneously aborted fetuses (Group I: five fetuses aged between 22 and 28 weeks of gestation; Group II: four fetuses aged between 32 and 38 weeks of gestation), characterized by a combination of histology, fluorescence and immunohistochemistry. In Group I, the posterior tibial tendon showed statistically significant greater cellularity and presence of collagen III and V than in Group II tendon, which showed a predominance of collagenous I and a better organization of the extracellular matrix compared with Group I tendons. In addition, a statistically significant higher rate of CD90, a marker of mesenchymal cells, was found in Group I tendons. In fetuses with gestational age between 22 and 28 weeks, the posterior tibialis tendons showed a thin and disorganized fibrillar structure, with an increase in collagen III and V fibers and mesenchymal cells. In the posterior tibialis tendons of fetuses with gestational age between 32 and 38 weeks, the fibrillar structure was thicker with a statistically significant increase in type I collagen and decreased cellularity.https://doi.org/10.1038/s41598-022-19695-3 |
| spellingShingle | Rodrigo Sousa Macedo Walcy Rosolia Teodoro Vera Luiza Capellozzi Dov Lagus Rosemberg Rafael Barban Sposeto Cesar de Cesar Netto Jonathan T. Deland Nicola Maffulli Scott J. Ellis Alexandre Leme Godoy-Santos Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons Scientific Reports |
| title | Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons |
| title_full | Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons |
| title_fullStr | Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons |
| title_full_unstemmed | Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons |
| title_short | Histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons |
| title_sort | histoarchitecture of the fibrillary matrix of human fetal posterior tibial tendons |
| url | https://doi.org/10.1038/s41598-022-19695-3 |
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