Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-Venezuela
A total of 79 S. aureus strains and 47 coagulase negative Staphylococcus (CNS) isolates from cow milk suffering subclinical mastitis were investigated for their ability to form biofilm as one of the most important virulence factors. Using Congo Red Agar method, 80% of S. aureus strains were sli...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2017-08-01
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| Series: | Kasmera |
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| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/22833 |
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| author | Kutchynskaya Valero-Leal Jhoandry Rivera-Salazar Wilfido Briñez América Paz Eyilde Piña Yeiny Ávila Gladis Colina López |
| author_facet | Kutchynskaya Valero-Leal Jhoandry Rivera-Salazar Wilfido Briñez América Paz Eyilde Piña Yeiny Ávila Gladis Colina López |
| author_sort | Kutchynskaya Valero-Leal |
| collection | DOAJ |
| description | A total of 79 S. aureus strains and 47 coagulase negative Staphylococcus (CNS) isolates from cow milk suffering subclinical mastitis were investigated for their ability to form biofilm as one of the most important virulence factors. Using Congo Red Agar method, 80% of S. aureus strains were slime producers, while in CNS was 32%. By microtiter plate method, 55%, 17%, and 28% of S. aureus isolates were strong, moderate, and weak biofilm producers, respectively, while in CNS the percentages were 43%, 17%, and 40%, respectively. All isolates were screened by Polymerase chain reaction (PCR) for amplification of intercellular adhesion gene A (icaA). In S. aureus isolates the icaA gene was present in 65 % while in CNS was 11%. The majority of S. aureus characterized in this study formed biofilm, which suggests that biofilm formation has an important role in the virulence of S. aureus isolated from bovine intramammary infections in Zulia state. |
| first_indexed | 2024-03-12T11:04:07Z |
| format | Article |
| id | doaj.art-108cfd806ffe4340abcd363633cf3b8c |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T11:04:07Z |
| publishDate | 2017-08-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-108cfd806ffe4340abcd363633cf3b8c2023-09-02T04:14:18ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282017-08-01451243224402Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-VenezuelaKutchynskaya Valero-Leal0Jhoandry Rivera-Salazar1Wilfido Briñez2América Paz3Eyilde Piña4Yeiny Ávila5Gladis Colina López6Bacteriología Clínica, Escuela de Bioanálisis, Facultad de Medicina. Universidad del Zulia- VenezuelaLaboratorio de Genética y Biología Molecular, Facultad Experimental de Ciencias. Universidad del Zulia- VenezuelaUnidad de Ciencia y Tecnología de Alimentos (UDICTA), Facultad de Ciencias Veterinarias. Universidad del Zulia- VenezuelaPráctica Profesional de Bacteriología, Escuela de Bioanálisis, Facultad de Medicina. Universidad del Zulia- VenezuelaProfesional de Bacteriología, Escuela de Bioanálisis, Facultad de Medicina. Universidad del Zulia- VenezuelaBacteriología General, Escuela de Bioanálisis, Facultad de Medicina. Universidad del Zulia- VenezuelaLaboratorio de Bacteriología Escuela de Bioanálisis, Facultad de Medicina. Universidad del Zulia- VenezuelaA total of 79 S. aureus strains and 47 coagulase negative Staphylococcus (CNS) isolates from cow milk suffering subclinical mastitis were investigated for their ability to form biofilm as one of the most important virulence factors. Using Congo Red Agar method, 80% of S. aureus strains were slime producers, while in CNS was 32%. By microtiter plate method, 55%, 17%, and 28% of S. aureus isolates were strong, moderate, and weak biofilm producers, respectively, while in CNS the percentages were 43%, 17%, and 40%, respectively. All isolates were screened by Polymerase chain reaction (PCR) for amplification of intercellular adhesion gene A (icaA). In S. aureus isolates the icaA gene was present in 65 % while in CNS was 11%. The majority of S. aureus characterized in this study formed biofilm, which suggests that biofilm formation has an important role in the virulence of S. aureus isolated from bovine intramammary infections in Zulia state.https://produccioncientificaluz.org/index.php/kasmera/article/view/22833MastitisStaphylococcus aureusStaphylococcus coagulasa negativabiopelículalimogen icaA |
| spellingShingle | Kutchynskaya Valero-Leal Jhoandry Rivera-Salazar Wilfido Briñez América Paz Eyilde Piña Yeiny Ávila Gladis Colina López Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-Venezuela Kasmera Mastitis Staphylococcus aureus Staphylococcus coagulasa negativa biopelícula limo gen icaA |
| title | Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-Venezuela |
| title_full | Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-Venezuela |
| title_fullStr | Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-Venezuela |
| title_full_unstemmed | Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-Venezuela |
| title_short | Biofilm formation and slime producing ability in Staphylococci causing subclinic bovine mastitis in the Zulia state-Venezuela |
| title_sort | biofilm formation and slime producing ability in staphylococci causing subclinic bovine mastitis in the zulia state venezuela |
| topic | Mastitis Staphylococcus aureus Staphylococcus coagulasa negativa biopelícula limo gen icaA |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/22833 |
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