A novel restrainer device for acquistion of brain images in awake rats
Functional neuroimaging methods like fMRI and PET are vital in neuroscience research, but require that subjects remain still throughout the scan. In animal research, anesthetic agents are typically applied to facilitate the acquisition of high-quality data with minimal motion artifact. However, anes...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
|
| Series: | NeuroImage |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S105381192400051X |
| _version_ | 1797289716270235648 |
|---|---|
| author | Jakov Tiefenbach Logan Shannon Mark Lobosky Sadie Johnson Hugh H Chan Nicole Byram Andre G Machado Charlie Androjna Kenneth B Baker |
| author_facet | Jakov Tiefenbach Logan Shannon Mark Lobosky Sadie Johnson Hugh H Chan Nicole Byram Andre G Machado Charlie Androjna Kenneth B Baker |
| author_sort | Jakov Tiefenbach |
| collection | DOAJ |
| description | Functional neuroimaging methods like fMRI and PET are vital in neuroscience research, but require that subjects remain still throughout the scan. In animal research, anesthetic agents are typically applied to facilitate the acquisition of high-quality data with minimal motion artifact. However, anesthesia can have profound effects on brain metabolism, selectively altering dynamic neural networks and confounding the acquired data. To overcome the challenge, we have developed a novel head fixation device designed to support awake rat brain imaging. A validation experiment demonstrated that the device effectively minimizes animal motion throughout the scan, with mean absolute displacement and mean relative displacement of 0.0256 (SD: 0.001) and 0.009 (SD: 0.002), across eight evaluated subjects throughout fMRI image acquisition (total scanning time per subject: 31 min, 12 s). Furthermore, the awake scans did not induce discernable stress to the animals, with stable physiological parameters throughout the scan (Mean HR: 344, Mean RR: 56, Mean SpO2: 94 %) and unaltered serum corticosterone levels (p = 0.159). In conclusion, the device presented in this paper offers an effective and safe method of acquiring functional brain images in rats, allowing researchers to minimize the confounding effects of anesthetic use. |
| first_indexed | 2024-03-07T19:10:01Z |
| format | Article |
| id | doaj.art-1093df406f454514a393e670f2455f7b |
| institution | Directory Open Access Journal |
| issn | 1095-9572 |
| language | English |
| last_indexed | 2024-03-07T19:10:01Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | NeuroImage |
| spelling | doaj.art-1093df406f454514a393e670f2455f7b2024-03-01T05:05:43ZengElsevierNeuroImage1095-95722024-04-01289120556A novel restrainer device for acquistion of brain images in awake ratsJakov Tiefenbach0Logan Shannon1Mark Lobosky2Sadie Johnson3Hugh H Chan4Nicole Byram5Andre G Machado6Charlie Androjna7Kenneth B Baker8Department of Neuroscience, Lerner Research Institute, Cleveland Clinic, OH 44195, USA; Corresponding author.Engineering Core, Lerner Research Institute, Cleveland Clinic, OH 44195, USASmall Animal Imaging Core, Lerner Research Institute, Cleveland Clinic, OH 44195, USAEngineering Core, Lerner Research Institute, Cleveland Clinic, OH 44195, USADepartment of Neuroscience, Lerner Research Institute, Cleveland Clinic, OH 44195, USACleveland Clinic Innovations, Cleveland Clinic, OH 44195, USADepartment of Neuroscience, Lerner Research Institute, Cleveland Clinic, OH 44195, USAEngineering Core, Lerner Research Institute, Cleveland Clinic, OH 44195, USADepartment of Neuroscience, Lerner Research Institute, Cleveland Clinic, OH 44195, USAFunctional neuroimaging methods like fMRI and PET are vital in neuroscience research, but require that subjects remain still throughout the scan. In animal research, anesthetic agents are typically applied to facilitate the acquisition of high-quality data with minimal motion artifact. However, anesthesia can have profound effects on brain metabolism, selectively altering dynamic neural networks and confounding the acquired data. To overcome the challenge, we have developed a novel head fixation device designed to support awake rat brain imaging. A validation experiment demonstrated that the device effectively minimizes animal motion throughout the scan, with mean absolute displacement and mean relative displacement of 0.0256 (SD: 0.001) and 0.009 (SD: 0.002), across eight evaluated subjects throughout fMRI image acquisition (total scanning time per subject: 31 min, 12 s). Furthermore, the awake scans did not induce discernable stress to the animals, with stable physiological parameters throughout the scan (Mean HR: 344, Mean RR: 56, Mean SpO2: 94 %) and unaltered serum corticosterone levels (p = 0.159). In conclusion, the device presented in this paper offers an effective and safe method of acquiring functional brain images in rats, allowing researchers to minimize the confounding effects of anesthetic use.http://www.sciencedirect.com/science/article/pii/S105381192400051XAwake imagingfunctional MRIFunctional imagingSmall animal imagingRestrainer deviceHead fixation device |
| spellingShingle | Jakov Tiefenbach Logan Shannon Mark Lobosky Sadie Johnson Hugh H Chan Nicole Byram Andre G Machado Charlie Androjna Kenneth B Baker A novel restrainer device for acquistion of brain images in awake rats NeuroImage Awake imaging functional MRI Functional imaging Small animal imaging Restrainer device Head fixation device |
| title | A novel restrainer device for acquistion of brain images in awake rats |
| title_full | A novel restrainer device for acquistion of brain images in awake rats |
| title_fullStr | A novel restrainer device for acquistion of brain images in awake rats |
| title_full_unstemmed | A novel restrainer device for acquistion of brain images in awake rats |
| title_short | A novel restrainer device for acquistion of brain images in awake rats |
| title_sort | novel restrainer device for acquistion of brain images in awake rats |
| topic | Awake imaging functional MRI Functional imaging Small animal imaging Restrainer device Head fixation device |
| url | http://www.sciencedirect.com/science/article/pii/S105381192400051X |
| work_keys_str_mv | AT jakovtiefenbach anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT loganshannon anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT marklobosky anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT sadiejohnson anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT hughhchan anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT nicolebyram anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT andregmachado anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT charlieandrojna anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT kennethbbaker anovelrestrainerdeviceforacquistionofbrainimagesinawakerats AT jakovtiefenbach novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT loganshannon novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT marklobosky novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT sadiejohnson novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT hughhchan novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT nicolebyram novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT andregmachado novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT charlieandrojna novelrestrainerdeviceforacquistionofbrainimagesinawakerats AT kennethbbaker novelrestrainerdeviceforacquistionofbrainimagesinawakerats |