Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue
Abstract Objective Chloride (Cl−) secretion is crucial for intestinal fluid secretion. Therefore, effects of the polyphenol Resveratrol (RSV) on Cl− secretion have been investigated. In a previous study, we observed effects of RSV on forskolin-induced Cl− secretion in the porcine jejunum but not the...
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| Format: | Article |
| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3825-4 |
| _version_ | 1818507785248178176 |
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| author | Susanne Hoppe Gerhard Breves Stefanie Klinger |
| author_facet | Susanne Hoppe Gerhard Breves Stefanie Klinger |
| author_sort | Susanne Hoppe |
| collection | DOAJ |
| description | Abstract Objective Chloride (Cl−) secretion is crucial for intestinal fluid secretion. Therefore, effects of the polyphenol Resveratrol (RSV) on Cl− secretion have been investigated. In a previous study, we observed effects of RSV on forskolin-induced Cl− secretion in the porcine jejunum but not the ileum although RSV itself induced a transepithelial ion current that may represent Cl− secretion in the ileum. The aim of this study was to gain further insights regarding the effects of RSV on characteristics of Cl− secretion in the porcine ileum using the Ussing chamber technique (recording of short circuit currents (Isc) as a measure for epithelial net ion transfer). Results RSV increased the Isc in the porcine ileum but not in the porcine jejunum as is already known. This increase was absent in a Cl−-free buffer system, indicating that RSV indeed induces Cl− secretion. However, the carbachol-induced Isc was significantly inhibited by RSV indicating an inhibition of Ca2+-induced Cl− secretion. The cellular basis for these contradictory, segment specific results of RSV on Cl− secretion has to be subjected to further studies. The results also underline, that is difficult to generalize effects of RSV between different intestinal locations, organs, cell culture models or species. |
| first_indexed | 2024-12-10T22:22:46Z |
| format | Article |
| id | doaj.art-1093e1d69fa148ae9ce32098f85e43ed |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-10T22:22:46Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-1093e1d69fa148ae9ce32098f85e43ed2022-12-22T01:31:15ZengBMCBMC Research Notes1756-05002018-10-011111610.1186/s13104-018-3825-4Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissueSusanne Hoppe0Gerhard Breves1Stefanie Klinger2Department of Physiology, University of Veterinary Medicine Hannover, FoundationDepartment of Physiology, University of Veterinary Medicine Hannover, FoundationDepartment of Physiology, University of Veterinary Medicine Hannover, FoundationAbstract Objective Chloride (Cl−) secretion is crucial for intestinal fluid secretion. Therefore, effects of the polyphenol Resveratrol (RSV) on Cl− secretion have been investigated. In a previous study, we observed effects of RSV on forskolin-induced Cl− secretion in the porcine jejunum but not the ileum although RSV itself induced a transepithelial ion current that may represent Cl− secretion in the ileum. The aim of this study was to gain further insights regarding the effects of RSV on characteristics of Cl− secretion in the porcine ileum using the Ussing chamber technique (recording of short circuit currents (Isc) as a measure for epithelial net ion transfer). Results RSV increased the Isc in the porcine ileum but not in the porcine jejunum as is already known. This increase was absent in a Cl−-free buffer system, indicating that RSV indeed induces Cl− secretion. However, the carbachol-induced Isc was significantly inhibited by RSV indicating an inhibition of Ca2+-induced Cl− secretion. The cellular basis for these contradictory, segment specific results of RSV on Cl− secretion has to be subjected to further studies. The results also underline, that is difficult to generalize effects of RSV between different intestinal locations, organs, cell culture models or species.http://link.springer.com/article/10.1186/s13104-018-3825-4CALCIUMcAMPCarbacholChloride secretionCFTRResveratrol |
| spellingShingle | Susanne Hoppe Gerhard Breves Stefanie Klinger Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue BMC Research Notes CALCIUM cAMP Carbachol Chloride secretion CFTR Resveratrol |
| title | Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue |
| title_full | Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue |
| title_fullStr | Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue |
| title_full_unstemmed | Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue |
| title_short | Calcium-induced chloride secretion is decreased by Resveratrol in ileal porcine tissue |
| title_sort | calcium induced chloride secretion is decreased by resveratrol in ileal porcine tissue |
| topic | CALCIUM cAMP Carbachol Chloride secretion CFTR Resveratrol |
| url | http://link.springer.com/article/10.1186/s13104-018-3825-4 |
| work_keys_str_mv | AT susannehoppe calciuminducedchloridesecretionisdecreasedbyresveratrolinilealporcinetissue AT gerhardbreves calciuminducedchloridesecretionisdecreasedbyresveratrolinilealporcinetissue AT stefanieklinger calciuminducedchloridesecretionisdecreasedbyresveratrolinilealporcinetissue |