The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers
Background/Aims: Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disease that significantly increases the risk of fetal complications. Here, we measured serum miRNA levels in ICP patients to identify candidate biomarkers for ICP. Methods: We used the Agilent miRNA array followed...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2018-11-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/495595 |
| _version_ | 1818930078778654720 |
|---|---|
| author | Ping Zou Liang Luo Chun Zhao Zhong Chen Ruirui Dong Na Li Yao Wang Jing Wang Tiejun Wang Minjian Chen Ting Zhang Daozhen Chen |
| author_facet | Ping Zou Liang Luo Chun Zhao Zhong Chen Ruirui Dong Na Li Yao Wang Jing Wang Tiejun Wang Minjian Chen Ting Zhang Daozhen Chen |
| author_sort | Ping Zou |
| collection | DOAJ |
| description | Background/Aims: Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disease that significantly increases the risk of fetal complications. Here, we measured serum miRNA levels in ICP patients to identify candidate biomarkers for ICP. Methods: We used the Agilent miRNA array followed by reverse transcription-polymerase chain reaction assays to identify and validate the serum miRNA profiles of 40 pregnant women with ICP and 40 healthy pregnant controls. We used bioinformatics to identify metabolic processes related to differentially expressed miRNAs. Results: The expression levels of three miRNAs (miR-371a- 5p, miR-6865-5p, and miR-1182) were significantly increased in ICP patients compared to controls; the areas under the receiver operating characteristic (ROC) curves (AUCs) were 0.771, 0.811, and 0.798, respectively. Multiple logistic regression analysis showed that a combination of the levels of the three miRNAs afforded a greater AUC (0.845), thus more reliably diagnosing ICP. The levels of all three miRNAs were positively associated with that of total bile acids. Furthermore, bioinformatics analysis indicated that the three miRNAs principally affected lipid phosphorylation, apoptosis, and the MAPK signaling pathway. Conclusion: This preliminary work improves our understanding of serum miRNA changes in pregnant women with ICP. The three miRNAs may serve as novel noninvasive biomarkers of ICP. |
| first_indexed | 2024-12-20T03:54:59Z |
| format | Article |
| id | doaj.art-109802651c7d47d89e149134f4ca5941 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-20T03:54:59Z |
| publishDate | 2018-11-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-109802651c7d47d89e149134f4ca59412022-12-21T19:54:22ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-11-015131480148810.1159/000495595495595The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive BiomarkersPing ZouLiang LuoChun ZhaoZhong ChenRuirui DongNa LiYao WangJing WangTiejun WangMinjian ChenTing ZhangDaozhen ChenBackground/Aims: Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific disease that significantly increases the risk of fetal complications. Here, we measured serum miRNA levels in ICP patients to identify candidate biomarkers for ICP. Methods: We used the Agilent miRNA array followed by reverse transcription-polymerase chain reaction assays to identify and validate the serum miRNA profiles of 40 pregnant women with ICP and 40 healthy pregnant controls. We used bioinformatics to identify metabolic processes related to differentially expressed miRNAs. Results: The expression levels of three miRNAs (miR-371a- 5p, miR-6865-5p, and miR-1182) were significantly increased in ICP patients compared to controls; the areas under the receiver operating characteristic (ROC) curves (AUCs) were 0.771, 0.811, and 0.798, respectively. Multiple logistic regression analysis showed that a combination of the levels of the three miRNAs afforded a greater AUC (0.845), thus more reliably diagnosing ICP. The levels of all three miRNAs were positively associated with that of total bile acids. Furthermore, bioinformatics analysis indicated that the three miRNAs principally affected lipid phosphorylation, apoptosis, and the MAPK signaling pathway. Conclusion: This preliminary work improves our understanding of serum miRNA changes in pregnant women with ICP. The three miRNAs may serve as novel noninvasive biomarkers of ICP.https://www.karger.com/Article/FullText/495595Intrahepatic cholestasis of pregnancyMicroRNANoninvasive biomarkerMicroarray |
| spellingShingle | Ping Zou Liang Luo Chun Zhao Zhong Chen Ruirui Dong Na Li Yao Wang Jing Wang Tiejun Wang Minjian Chen Ting Zhang Daozhen Chen The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers Cellular Physiology and Biochemistry Intrahepatic cholestasis of pregnancy MicroRNA Noninvasive biomarker Microarray |
| title | The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers |
| title_full | The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers |
| title_fullStr | The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers |
| title_full_unstemmed | The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers |
| title_short | The Serum microRNA Profile of Intrahepatic Cholestasis of Pregnancy: Identification of Novel Noninvasive Biomarkers |
| title_sort | serum microrna profile of intrahepatic cholestasis of pregnancy identification of novel noninvasive biomarkers |
| topic | Intrahepatic cholestasis of pregnancy MicroRNA Noninvasive biomarker Microarray |
| url | https://www.karger.com/Article/FullText/495595 |
| work_keys_str_mv | AT pingzou theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT liangluo theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT chunzhao theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT zhongchen theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT ruiruidong theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT nali theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT yaowang theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT jingwang theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT tiejunwang theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT minjianchen theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT tingzhang theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT daozhenchen theserummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT pingzou serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT liangluo serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT chunzhao serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT zhongchen serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT ruiruidong serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT nali serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT yaowang serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT jingwang serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT tiejunwang serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT minjianchen serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT tingzhang serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers AT daozhenchen serummicrornaprofileofintrahepaticcholestasisofpregnancyidentificationofnovelnoninvasivebiomarkers |